Method for preparing squalene by using lignocellulose

Abstract

Aiming at the problems existing in the production of squalene by microbial fermentation in the prior art, the invention provides a process for preparing squalene by using lignocellulosic agricultural and forestry wastes as raw materials. The process includes the steps of pretreatment, saccharification, yeast-like fermentation, squalene extraction and the like. The process adopts the combination of lignocellulosic biosaccharification and yeast-like high-density fermentation; by using lignocellulosic biomass as raw material, on the one hand, the cost of enzymes used in the saccharification stage and the production cost of squalene microbial fermentation are significantly reduced On the other hand, it also solves the problem of comprehensive utilization of agricultural and forestry waste. In addition, the lignocellulose saccharification stage medium and fermentation medium can be recycled, which can significantly save water and reduce the use of chemicals, and has a significant effect of reducing waste water discharge and reducing costs. In the process of the invention, the squalene output can reach 13g / L, accounting for 5-9% of the dry weight of the bacteria, and the purity of the refined squalene is greater than 93%.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a method for producing squalene by using lignocellulose raw materials. Background technique [0002] Squalene is a non-toxic marine biologically active substance with the effect of preventing and curing diseases. Its chemical name is 2,6,10,15,19,23-hexamethyl-2,6,10,14 ,18,22-Tetracosahexaene has many physiological functions such as increasing the activity of superoxide dismutase in the body, enhancing the body's immunity, improving sexual function, anti-aging, anti-fatigue, anti-tumor, etc. It is widely used in medicine, Beauty, cosmetics, food and other fields. [0003] Currently, the main source of squalene is fish oil, especially shark liver oil. However, with the depletion of fishery resources and the improvement of relevant regulations, people are trying to find other sources of squalene; such as from olive oil, palm oil and other oil crops, or from amar...

AI Technical Summary

Problems solved by technology

However, since the activity and stability of exogenously added proteins will decrease with the progress of the saccharification process, the consumption of enzymes requires an increase in the amount or frequency of enzyme addition

In addition, these non-cellulosomal proteins are added to the saccharification system as free enzymes, which cannot interact with cellulosomes, and will significantly reduce the synergistic effect with other enzymes in the system, inevitably resulting in additional enzymes that are greater than the demand, resulting in The high cost of enzyme preparations

Not only that, this saccharification strategy that relies on the addition of exogenous free enzymes has complex processes, high equipment requirements, and conversion efficiency that cannot meet the requirements of industrial production.

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