Ginsenoside CK preparation method

A technology of ginsenosides and total saponins, applied in chemical instruments and methods, glycoside steroids, steroids, etc., can solve the problems of large pollution of chemical methods, many by-products of hydrolysis, serious problems, etc., and achieves easy separation and removal of impurities and conversion efficiency. High and low cost effect

Active Publication Date: 2018-11-30
YUNNAN YUNKE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional thinking is to obtain products by using acid-base hydrolysis, but chemical methods have disadvantages such as large pollution, high cost, and many hydrolysis by-products; chemical methods are difficult to control products, and it is difficult to obtain a single target product, and a large number of separations are required in the follow-up process, serious environmental pollution and other disadvantages
Therefore, relevant researches are decreasing year by year, and now they are mostly concentrated on biotransformation methods, mainly including microbial transformation and enzymatic transformation; the conversion efficiency of ginsenoside β-glucosidase isolated from ginseng in literature reports is relatively high, and there are few impurities, but The enzyme is not a single compound, it is difficult to artificially prepare, and the cost of direct separation is high, so it is difficult to apply to the industrialization of the enzymatic hydrolysis preparation method of ginsenoside CK

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: The preparation method steps of ginsenoside CK are as follows:

[0019] (1) Take Panax notoginseng leaves as raw material, after crushing, use methanol solution with a mass concentration of 50% to reflux extract three times, the extraction temperature is 80°C, and the extraction time is 2 hours each time, filter, combine the extracts, concentrate under reduced pressure to recover the organic solvent, Get the crude extract of Panax notoginseng leaves;

[0020] (2) Pass the crude extract of Notoginseng leaf in step (1) through a column of styrene macroporous adsorption resin D101 to absorb the total saponins of Notoginseng leaf, wash the resin column with water until it is colorless, and then analyze Notoginseng leaf with an ethanol solution with a volume concentration of 70% Total saponins, collect the eluate, recover ethanol, concentrate and dry to obtain the total saponins of Panax notoginseng;

[0021] (3) Add absolute ethanol 4 times its mass to the to...

Embodiment 2

[0026] Embodiment 2: The preparation method steps of ginsenoside CK are as follows:

[0027] (1) Use Panax notoginseng leaves as raw material, after pulverization, heat reflux extraction with methanol solution with a mass concentration of 60% for 2 times, the extraction temperature is 85°C, and the extraction time is 1.5h each time, filter, combine the extracts, and concentrate under reduced pressure to recover the organic solvent , to obtain the crude extract of Panax notoginseng leaves;

[0028](2) Pass the crude extract of Notoginseng leaves in step (1) through a styrene macroporous adsorption resin HPD100 column to absorb the total saponins of Notoginseng leaves, wash the resin column with water until it is colorless, and then analyze Notoginseng leaves with an ethanol solution with a volume concentration of 72% Total saponins, collect the eluate, recover ethanol, concentrate and dry to obtain the total saponins of Panax notoginseng;

[0029] (3) Add methanol 5 times its ...

Embodiment 3

[0034] Embodiment 3: The preparation method steps of ginsenoside CK are as follows:

[0035] (1) Take Panax notoginseng leaves as raw material, after pulverization, use ethanol solution with a mass concentration of 70% to heat-reflux extract twice, the extraction temperature is 85°C, and the extraction time is 1h each time, filter, combine the extracts, concentrate under reduced pressure to recover the organic solvent, Get the crude extract of Panax notoginseng leaves;

[0036] (2) Pass the crude extract of Notoginseng leaves in step (1) through a styrene macroporous adsorption resin XAD-1600 column to absorb the total saponins of Notoginseng leaves, wash the resin column with water until it is colorless, and then use an ethanol solution with a volume concentration of 75% to analyze the three Aescin total saponins, collect the eluate, recover ethanol, concentrate and dry to obtain total aescin;

[0037] (3) Add n-butanol 6 times its mass to the total saponins of Notoginseng l...

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PUM

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Abstract

The invention discloses a ginsenoside CK preparation method. According to the present invention, ginsenoside CK is prepared by using a mixed acid hydrolysis method, wherein the ginsenoside CK with thecontent of more than 98% is prepared through preparation of the total saponin of the leaves of Panax notoginseng, preparation of ginsenoside CK, purification and other steps; the conversion efficiency of the method is high, the conversion product is easily separated so as to remove the impurity, and the ginsenoside CK crude product with the content of more than 90% can be obtained by using the simple solvent treatment, such that the disadvantage that the ginsenoside CK can be obtained by repeatedly using the silica gel column and gel column chromatography method is avoided; and the method hasadvantages of low cost and high efficiency, and is suitable for industrial production and marketing application.

Description

technical field [0001] The invention relates to a preparation method of ginsenoside CK, belonging to the field of extract preparation. Background technique [0002] Ginsenoside CK is the metabolite of diol-type ginsenoside in ginseng in the human intestine, and it is the entity that ginseng exerts activity in the body; biological activity studies have shown that ginsenoside CK is a multi-target, highly active compound, which not only has anti-tumor , Anti-inflammation, liver protection and anti-allergic aspects have shown good activities, and also have a good regulatory effect on the nervous system and immune system, and can inhibit tumor cell proliferation and inflammation, anti-carcinogenic and anti-cancer, anti-allergic, relieve Symptoms of Alzheimer's disease, anti-diabetes and antioxidant factors promote the secretion of isulin, protect the liver and skin. [0003] The stability of ginsenoside CK is poor, and ginsenoside does not exist in natural ginseng. The outstandi...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 辛文锋蔡群虎刘石磊张文生胡会泽袁敏惠王芬张芳盛郭霞余斌孟维
Owner YUNNAN YUNKE PHARMA
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