Method for extracting okra pectin by high-voltage pulse
A high-voltage pulse and pectin technology, applied in the field of pectin extraction, can solve the problems of low extraction rate, high cost, and poor quality of extracted products, and achieve the effect of increasing extraction rate, increasing value, and easy operation
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Embodiment 1
[0021] The method for high-voltage pulse extraction of okra pectin in the present embodiment, concrete steps are as follows:
[0022] 1) Preparation: Take 1 kg of fresh and disease-free destemmed okra tender fruit, clean it, put the tender okra fruit at 85°C for 60 seconds, then wash it with water for 3 times to remove pigment and sugar. Dried to a water content of 20% by blowing air, pulverized and passed through a 100-mesh sieve to obtain okra fruit powder;
[0023] 2) High-pressure pulse treatment: add okra fruit powder to the enzyme-containing extract with an enzyme content of 8000u / g mixed with pectinase and cellulase at a mass ratio of 1.5:1, and keep the solid-liquid ratio at 1:9 , the pH of the mixed solution is between 6-7, and then the mixed solution is placed in a constant temperature water bath at 45°C, stirred and extracted for 35 minutes after the rotation speed is 25r / min, and then water is added to adjust the ratio of solid to liquid to 1:23 and 8% of The citr...
Embodiment 2
[0026] This example is the same as Example 1, except that the electric field strength of the high-voltage pulse electric field treatment in this example is 10kv / cm, the number of pulses is 5, the temperature is 55°C, and the adjusted solid-liquid ratio is 1:25.
Embodiment 3
[0028] This example is the same as Example 1, except that the electric field strength of the high-voltage pulse electric field treatment in this example is 20kv / cm, the number of pulses is 10, the temperature is 65°C, and the adjusted solid-liquid ratio is 1:18.
[0029] The okra pectin prepared in Example 1-3 is weighed to calculate the extraction rate, and the calculation formula of the extraction rate=(okra pectin weight / weight of okra tender fruit)×100%, the okra prepared in the calculation example 1-3 The extraction rate of pectin is all at 20.1%, and wherein the extraction rate of embodiment 1 is the highest 23.5%.
[0030] The extracted okra is a light yellow solid with no peculiar smell. It can be dissolved in 20 times water to form a viscous liquid. Take 1.0 g of dried pectin powder, add 50 mL of purified water, and stir continuously with a glass rod, and a viscous liquid can be observed.
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