Electrochemical sensor based on metal organic framework-vertical graphene composite structure and preparation method thereof

A metal-organic framework and graphene composite technology, applied in the field of biosensors, can solve the problems of difficulty in performance improvement, relatively large performance limitations of electrochemical sensors, weak conductivity of transition metal complexes, etc., to achieve optimal sensing performance and conduction Performance, possibility of large space extension, effect of increasing adsorption point of reactants

Inactive Publication Date: 2018-12-11
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] First, among the carbon nanomaterials, carbon nanotubes, graphene, acetylene black, and carbon nanofibers all have the general characteristics of electrochemical sensors, but the functions of these carbon nanomaterials are relatively single. The performance improvement of the sensor has relatively large limitations, and the response rate still cannot meet the requirements of medical applications;
[0007] Second, metal and metal oxide nanoparticles, similar to carbon nanomaterials, are composed of a single conductive material, which is not conducive to the development of electrochemical sensors in a more diverse direction, and cannot be both conductive and sensitive. Extraordinary ability;
[0008] Third, transition metal complexes play an important role in the field of electrochemical sensing, but transition metals need to be combined with other substances to form complexes. Only the limitations of transition metals themselves are relatively large, and they are still relatively conductive compared to graphene. As far as excellent materials are concerned, the conductivity of transition metal complexes is still relatively weak;
[0009] Fourth, the conductive polymer modified electrode has the advantages of high active group concentration, accelerated electron transfer, large chemical influence signal, and good stability, bu

Method used

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  • Electrochemical sensor based on metal organic framework-vertical graphene composite structure and preparation method thereof
  • Electrochemical sensor based on metal organic framework-vertical graphene composite structure and preparation method thereof
  • Electrochemical sensor based on metal organic framework-vertical graphene composite structure and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0053] Embodiment 1: electrochemical detection NO standard solution

[0054] (1) Using 4.0×10 containing 0.1M KCl -3 M Fe[(CN) 6 ] 3- / 4- The solution is an electrolyte, and the electrochemical properties of ZIF-8@vG electrode materials are characterized by cyclic voltammetry and electrochemical impedance spectroscopy;

[0055] (2) Using 2-(N,N-diethylamino)-diazene-2-oxysodium salt hydrate as a precursor to produce NO, prepare NO standard solution;

[0056] (3) Use the Griess test to determine the concentration of NO in the precursor solution: Nafion was coated on the surface of the ZIF-8@vG composite electrode to improve its specificity. Using cyclic voltammetry to detect different concentrations of NO standard solutions, after determining the best working potential, use chronoamperometry to detect a series of NO solutions with gradient concentrations.

[0057] (4) The electrochemical device constructed by Nafion-coated ZIF-8@vG composite working electrode was tested by a...

Embodiment 2

[0059] First, use polydimethylsiloxane (PDMS) to construct a small cell culture pool on the ZIF-8@vG substrate, and sterilize it with ultraviolet light for more than 30 minutes;

[0060] Then, respectively, will contain approximately 10 5 cells / mL The suspension of PC-12 cancer cells and common HEK-293 cells was added to the culture pool, using DMEM medium (containing 10% fetal bovine serum and 1% antibiotics), in a cell culture box with 5% CO 2 , cultured at 37°C to allow the cells to grow normally on the surface of the electrode material, and the chronoamperometry was used to test the ampere current response curve of the device after adding the polysaccharide solution.

[0061] Finally, cytotoxicity test: After the cells grow normally on the ZIF-8@vG substrate, they are stained with Calcein AM / PI staining agent to evaluate the viability of the cells and ensure that the electrode material will not be toxic to the cells.

Embodiment 3

[0063] (1) Grow vertical graphene on the metal microneedle array, and then prepare ZIF-8@vG nanocomposite structure;

[0064] (2) Integrate the endoscope with the electrochemical sensor-microneedle system to prepare an integrated insertable chip;

[0065] (3) Insert microneedles into mice with tumors, and monitor the concentration of NO in normal cells and cancer cells in the mice.

[0066] Detection of NO concentrations in different tissues and organ sites in mice;

[0067] (4) Effects of injection of different drugs on NO concentration in various tissues and organs.

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Abstract

The invention belongs to the technical field of biosensors, and in particular discloses an electrochemical sensor based on a metal organic framework-vertical graphene composite structure and a preparation method thereof. The preparation method specifically comprises the following steps: (1) growing vertical graphene on a stainless steel substrate; (2) synthesizing various different metal organic framework (MOF for short) materials such as ZIF-8, KAUST-1 and Ni-MIL-77 by taking the vertical graphene on the stainless steel substrate as a template; and (4) constructing the electrochemical sensorby taking the MOF-vertical graphene composite nanostructure as an electrode material. The electrochemical sensor has higher electric conductivity, larger specific surface area and more types of responsive substance surfaces, and the preparation method is relatively clear, different MOFs can be made by selecting materials according to performance of chemical reactions directly, and the spatial structure of the MOF material can be altered according to performance demand, so that the electrochemical sensor which further meets the demand can be manufactured more easily, and is wide in applicationrange.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to an electrochemical sensor based on a metal organic framework-vertical graphene composite structure and a preparation method thereof. Background technique [0002] A biosensor is a device used to monitor a living system or a biochemical substance associated with it. Because of its advantages such as good selectivity, high sensitivity, fast analysis speed, simple and low-cost instruments, it is an important part of current scientific research. It has been highly valued and widely used in clinical diagnosis, environmental monitoring, drug screening, food safety and agricultural testing. Among them, electrochemical sensors are frequently used in limited space applications due to their advantages of simple operation, low cost, fast response, and high sensitivity. By improving the performance of some sensitive functional materials, it is of great significance for the development of ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/42
CPCG01N27/3278G01N27/42
Inventor 刘繁茂黄爽何根杨鑫杭天陈惠琄杨成端金全昌文瑞陶军谢曦
Owner SUN YAT SEN UNIV
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