Nano electrochemical enzyme sensor for detecting trichloroacetic acid or sodium nitrite as well as preparation method and application of nano electrochemical enzyme sensor

An enzyme sensor, sodium nitrite technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve the effect of long service life, stable spatial configuration, and favorable charge transmission

Active Publication Date: 2018-12-11
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its appearance and taste are similar to table salt. It is widely used in industry and construction. It is also allowed to be used as a coloring agent in meat products in limited quantities. However, because nitrite is a carcinogen, it has a higher chance of causing food poisoning. So the implementation for NaNO 2 It is particularly important to detect highly sensitive

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  • Nano electrochemical enzyme sensor for detecting trichloroacetic acid or sodium nitrite as well as preparation method and application of nano electrochemical enzyme sensor
  • Nano electrochemical enzyme sensor for detecting trichloroacetic acid or sodium nitrite as well as preparation method and application of nano electrochemical enzyme sensor
  • Nano electrochemical enzyme sensor for detecting trichloroacetic acid or sodium nitrite as well as preparation method and application of nano electrochemical enzyme sensor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] One for detecting TCA or NaNO 2 The nano-electrochemical enzyme sensor, including CILE used as the substrate electrode in the three-electrode system, the surface of CILE is modified with a composite film, the composite film is composed of Mg-MOFs-74, AuNPs, Mb and Nafion arranged in sequence from the inside to the outside .

[0039] When the above-mentioned nanometer electrochemical enzyme sensor is placed in the PBS buffer solution containing TCA with a pH of 2.0, the cyclic voltammetry curve is recorded, and a linear regression equation is constructed according to the relationship between the concentration of TCA and the reduction peak current, which can be further used in the solution to be tested. Detection of TCA concentration.

[0040] see figure 1 , which is the overlay of the cyclic voltammetry curves when the above-mentioned electrochemical enzyme sensor detects 4, 10, 20, 30, 40, 50, 60, 80, 100, 120, 150, 170, 200 mmol / L TCA solutions respectively, obviousl...

Embodiment 2

[0045] One of the above is used to detect TCA or NaNO 2 The preparation method of the nanometer electrochemical enzyme sensor, comprises the following steps:

[0046] S1, get 1.6 g graphite powder and 0.8 g N-hexylpyridine hexafluorophosphate (HPPF 6 ) was ground in a mortar for 2.5 h to obtain a carbon paste, and then the carbon paste was filled into a glass electrode tube with an inner diameter of 4 mm for compaction, and a copper wire was inserted as a wire to obtain CILE. 0.5% wt Nafion ethanol solution was drip-coated on the surface of CILE, and Nafion / CILE was obtained after standing to dry. image 3 Shows the cyclic voltammogram of Nafion / CILE measured in PBS buffer at pH 2.0;

[0047] S2. Modified Mg-MOFs-74: Drop-coat 0.3 mg / mL Mg-MOFs-74 suspension on the surface of CILE obtained in step S1 to obtain Mg-MOFs-74 modified CILE, namely Mg-MOFs-74 / CILE . 0.5%wt Nafion ethanol solution was drop-coated on the surface of Mg-MOFs-74 / CILE, and Nafion / Mg-MOFs-74 / CILE was o...

Embodiment 3

[0052] A kind of application of the nanometer electrochemical enzyme sensor of embodiment 1 in detecting TCA, comprises the following steps: with Nafion / AuNPs / Mg-MOFs-74 / CILE as working electrode, Ag / AgCl electrode as reference electrode, platinum electrode as For the electrode, a three-electrode system was established, and the PBS buffer solution with a pH of 2.0 was used as the supporting electrolyte, and the cyclic voltammetry curve was recorded; a detection linear regression equation was constructed according to the relationship between the TCA concentration and the reduction peak current, and the solution to be tested was calculated according to the linear regression equation TCA concentration in .

[0053] refer to Figure 4 , the detection linear regression diagram of the relationship between TCA concentration and reduction peak current. From the figure, it can be seen that the detection linear regression equation is: Ipc(μA)=3.399C(mmoL / L)+147.8, the correlation coeffi...

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Abstract

The invention discloses a nano electrochemical enzyme sensor for detecting trichloroacetic acid (TCA) or sodium nitrite (NaNO2) as well as a preparation method and application of the nano electrochemical enzyme sensor. A base electrode selected by the nano electrochemical enzyme sensor is a carbon ionic liquid electrode (CILE), a composite film composed of a magnesium metal-organic frameworks nanometer material (Mg-MOFs-74), gold nanoparticles (AuNPs) and muscle hemoglobin (Mb) is modified on the surface of the CILE, and a Nafion film is assembled on the surface of the composite film. The preparation method comprises the following steps: modifying the Mg-MOFs-74, performing electro-deposition on the AuNPs, assembling the Mb and Nafion film and the like. The nano electrochemical enzyme sensor capable of being used for detecting the TCA or NaNO2 is constructed in the invention, is capable of respectively detecting two target substances, is wide in detection range and low in detection limit, and can be used for determining samples containing the TCA or NaNO2.

Description

technical field [0001] The invention relates to the technical fields of nanomaterials, electrochemical detection and biosensors, in particular to a nanometer electrochemical enzyme sensor for detecting trichloroacetic acid or sodium nitrite and its preparation method and application. Background technique [0002] Electrochemical biosensors refer to sensors that use immobilized biological materials as sensitive elements. Its working principle is that biological components (enzymes, antigens, antibodies, etc.) or the organism itself (cells, tissues, organelles, etc.) are used as sensitive elements, and electrodes (solid electrodes, ion selective electrodes, gas sensitive electrodes, etc.) are used as conversion elements. , A sensor that uses potential, current or conductance as a characteristic detection signal. Electrochemical biosensors occupy an important proportion in biosensors, and their research has made rapid progress in recent years. [0003] Metal-Organic Framework...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/42
CPCG01N27/3271G01N27/42
Inventor 孙伟罗贵铃黄雅琦邹如意牛燕燕翁文举
Owner HAINAN NORMAL UNIV
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