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Method for simultaneous quantitative detection of arsenic and mercury forms in aquatic products

A quantitative detection method and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that affect the analysis of arsenic and mercury detection results, the same detection technology of arsenic and mercury forms that are not found, and unsatisfactory analysis results, etc. To achieve the effect of coping with export technical barriers, shortening testing time, and promoting consumer safety

Inactive Publication Date: 2018-12-11
ZHOUSHAN INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] To sum up, our country has opened the speciation analysis method in food element detection technology. However, after the implementation of the new standard, the scientific research team of our institute has conducted a large number of sample detection studies and found that the standard method of this type of project has a narrow scope of application. , low extraction efficiency, long detection time, unsatisfactory analysis results, etc., which affect the analysis of the detection results of arsenic and mercury
At the same time, after reviewing the relevant literature, basically only studied the speciation analysis level of arsenic and mercury, and found no co-measurement technology for the speciation of arsenic and mercury.

Method used

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  • Method for simultaneous quantitative detection of arsenic and mercury forms in aquatic products
  • Method for simultaneous quantitative detection of arsenic and mercury forms in aquatic products
  • Method for simultaneous quantitative detection of arsenic and mercury forms in aquatic products

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Embodiment 1 Simultaneous quantitative detection method of arsenic and mercury forms in aquatic products

[0050] 1. Experimental part

[0051] 1. Reagents and instruments

[0052] Ammonium dihydrogen phosphate (AR, Sinopharm Chemical Reagent Co., Ltd.),

[0053] Tetrabutylammonium hydroxide (Alfa, aq soln),

[0054] L-cysteine ​​(BR, Sinopharm Chemical Reagent Co., Ltd.),

[0055] Ammonium formate (AR, Sinopharm Chemical Reagent Co., Ltd.),

[0056] Methanol (HPLC grade, Merck, Germany),

[0057] Gold standard solution (1000mg / L, purchased from O2SI),

[0058] Arsenite (1000mg / L, purchased from O2SI),

[0059] Arsenate (1000mg / L, purchased from O2SI),

[0060] Monomethyl arsenic (1000mg / L, purchased from O2SI),

[0061] Dimethyl arsenic (1000mg / L, purchased from O2SI), 1

[0062] Methylmercury (1000mg / L, purchased from O2SI),

[0063] Ethylmercury (1000mg / L, purchased from O2SI).

[0064] HPLC (PE 200 series liquid chromatograph, American PE company),

[00...

Embodiment 2

[0099] Embodiment 2 method detection limit and the linear relationship of standard curve

[0100]Using mobile phase A as a solvent, prepare four arsenic mixed standard solutions with concentrations of 0ug / L, 5ug / L, 10ug / L, 20ug / L, 50ug / L, and 100ug / L. 2ug / L, 4ug / L, 6ug / L, 8ug / L, 10ug / L mixed standard solutions of two mercury elements. Depend on Figure 2-7 It can be seen that the correlation coefficients of the linear equations of each component are above 0.995, which meets the detection requirements. The retention time, detection limit and linear correlation coefficient of each form are shown in Table 1.

[0101] Table 1 Linear equation of simultaneous detection method of arsenic and mercury elements

[0102] Elemental form

Embodiment 3

[0103] Embodiment 3 standard addition recovery and precision experiment

[0104] The present invention selects 4 kinds of representative aquatic products (small yellow croaker, shrimp, swimming crab, jellyfish) etc. that are often eaten along the coast of Zhejiang to carry out sample processing according to the method in Example 1, and carry out the standard addition experiment with 3 different concentration levels (Table 2 ), each level was repeated 6 times, and the arsenic form (arsenous acid, dimethyl arsenic, monomethylarsenic acid, arsenic acid) and mercury form (methylmercury, ethylmercury) in aquatic products were determined, and the recovery rate The accuracy and precision of the method were examined by the situation and repeatability. The results are shown in Table 2. When the spiked concentration of arsenic form was 0.05mg / kg, 0.1mg / kg, and 0.3mg / kg, the recoveries ranged from 88.6% to 99.4%, and the relative standard deviation (RSD 0.40 %~4.27%); when the spiked co...

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Abstract

The invention discloses a method for simultaneous quantitative detection of arsenic and mercury forms in aquatic products. In the method, the HPLC (high performance liquid chromatography)-ICP (inductively coupled plasma)-MS (mass spectrometry) combination technology is adopted to detect 4 kinds of arsenic forms and 2 kinds of mercury forms in the aquatic products. Specific to the defects of samplepretreatment technology, the method plans to set up a kind of combination technology by which a new type of efficient pretreatment system is combined with the high performance liquid chromatography-inductively coupled plasma mass spectrometer, the method has high sensitivity and high selectivity and can be used for quickly analyzing the arsenic and mercury forms and simultaneously detecting analytic technology so as to perfect a detection technology for simultaneously detecting multiple elements and a standard method.

Description

technical field [0001] The invention belongs to the technical field of aquatic product detection, and in particular relates to a method for quantitatively detecting the forms of arsenic and mercury in aquatic products simultaneously. Background technique [0002] In recent years, food safety has become a topic of global concern. Food safety not only refers to food pollution problems such as pesticide residues in the usual sense, but also includes the harmful concentration thresholds of trace elements and the maintenance of harmful element forms for human health. The important phenomenon of trace element safety problems in food includes that once toxic trace elements enter the food chain through various channels, the total amount of harmful elements and their different forms in food may cause serious food safety problems. [0003] For example, mercury is divided into inorganic states (liquid elemental Hg, Hg 2+ and Hg + ) and organic forms (methylmercury, ethylmercury, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王维洁陈朋王萍亚赵巧玲戴意飞
Owner ZHOUSHAN INST FOR FOOD & DRUG CONTROL