A kind of bacillus amyloliquefaciens and application thereof
A technology of amylolytic spores and bacillus, applied in the field of microorganisms, can solve plant diseases caused by root rot, rice bacterial blight, sweet potato stem rot, tomato Ralstonia solanacearum and cucumber horn spot fungus and rice blast fungus , rice sheath blight, wheat scab, botrytis cinerea, grape canker, grape white rot and grape anthracnose
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Embodiment 1
[0024] Example 1: Screening of Bacillus amyloliquefaciens hzq1601
[0025] Sample collection: The sample collected was the rhizosphere soil of rice variety (Yongyou 12), and the location was the greenhouse nursery of Zhejiang Academy of Agricultural Sciences. Pull it out together with the rice root system and bring it back to the laboratory for isolation.
[0026] Isolation of Bacillus amyloliquefaciens: Weigh 1g of rhizosphere soil, put it into 100mL sterile water, shake and mix at room temperature for 1 hour, use sterile water according to 10, 10 2 、10 3 For gradient dilution, use a micropipette to take 100ul of the gradient dilution solution and spread it evenly on the LB solid medium plate. After cultivating in a 28°C incubator for 24 hours, pick up a single colony on the LB solid plate with an inoculation loop and culture it with an inoculation loop to obtain Purified bacterial single colonies were cultured with NA liquid medium at 28°C and shaken at 200r / min for 24 hou...
Embodiment 2
[0030] Embodiment 2: bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) identification of hzq1601
[0031] Such as figure 1 As shown, the Bacillus amyloliquefaciens hzq1601 screened in Example 1 was off-white on LB medium, opaque, dry and shrunken on the surface, with irregular edges, strong adhesion and fluidity, and Gram staining was positive , perinatal flagella, with spores, rod-shaped; this strain can grow on the surface of the medium and along the puncture line, and is a facultative anaerobic bacterium; it can produce catalase and starch hydrolase, and decompose gelatin And peptone to get tryptophan, but can not decompose sulfur-containing organic compounds; can reduce nitrate ions in the medium.
[0032] According to the morphological observation of the Bacillus amyloliquefaciens hzq1601 combined with the physiological and biochemical characteristics, the Bacillus amyloliquefaciens hzq1601 was preliminarily identified as a bacillus.
[0033] Molecular identi...
Embodiment 3
[0035] Embodiment 3: bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) Preparation of hzq1601 antibacterial agent
[0036] First, prepare the NA medium slant, inoculate the Bacillus amyloliquefaciens hzq1601 obtained in Example 1, put it into a 28°C incubator and cultivate it for 48 hours; secondly, configure the NA liquid medium, and divide it into Erlenmeyer flasks (250mL, 100mL liquid), sterilized at 121°C for 15 minutes, and after cooling, wash the above-mentioned slant bacteria directly with sterile water on the ultra-clean workbench, put them into the above-mentioned Erlenmeyer flask containing the sterilized NA liquid medium, and shake the flask Cultivate, pH 7.2, 28°C, 200r / min, cultivate for 48 hours to obtain shake flask culture bacteria, and the obtained fermentation liquid is Bacillus amyloliquefaciens hzq1601 antibacterial agent.
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