Microbial agent with high salt tolerance and high oil degradation rate, and preparation method thereof
A microbial agent and salt-tolerant technology, which is applied in the field of microbial agent and its preparation, can solve the problems of poor product maturity, long fermentation cycle, and low degradation rate of food waste oil, so as to reduce oil content and purchase cost effect
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preparation example Construction
[0038] See figure 1 , the preparation method of the microbial bacterial agent with high salt resistance and oil degradation rate of the present invention, comprises the following steps:
[0039] S1. Provide raw materials and culture media for strains. Raw materials for strains include Bacillus phlei, Bacillus amyloliquefaciens, plant lactic acid bacteria, Saccharomyces cerevisiae, Bacillus megaterium, Streptomyces albicans and Aspergillus oryzae; culture media include solid media, liquid Medium and fermentation medium, wherein, solid medium includes beef extract peptone agar medium, potato dextrose agar medium and lactic acid bacteria solid medium; liquid medium includes beef extract peptone liquid medium, potato dextrose liquid medium and lactic acid bacteria Bacterial liquid culture medium; fermentation medium is: molasses 3%, corn steep liquor powder 8%, magnesium sulfate 0.2%, sodium hydroxide 0.15%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.1%,...
Embodiment 1
[0049] S1. Slope culture: Under sterile conditions, inoculate Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus megaterium on beef extract peptone agar medium for cultivation, and inoculate plant lactic acid bacteria on lactic acid bacteria solid medium (MRS) For culturing, Saccharomyces cerevisiae, Streptomyces cerevisiae and Aspergillus oryzae are inoculated in Potato Dextrose Agar Medium (PDA) for cultivation, wherein plant lactic acid bacteria, Saccharomyces cerevisiae, Bacillus megaterium, Streptomyces cerevisiae and Aspergillus oryzae , Bacillus subtilis and Bacillus amyloliquefaciens were cultured at 30°C for 2 days;
[0050] S2. Primary seed culture: Under aseptic conditions, single colonies were picked and inoculated in 50mL / 250mL liquid medium from the strains cultivated in step S1. Aspergillus was cultured on a shaker at 160r / min for 2 days at 30°C, Bacillus subtilis and Bacillus amyloliquefaciens were cultured on a shaker at 150r / min for 2 days at 30°C, an...
Embodiment 2
[0056] The preparation method of this example is basically similar to that of Example 1, the difference is that the inoculum amount in step S3 is changed from 5% to 4%, and the high-salt and high-fat kitchen waste treatment microbial bacterial agent of this example is obtained. The bacterial composition is:
[0057]
PUM
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