Microbial agent with high salt tolerance and high oil degradation rate, and preparation method thereof

A microbial agent and salt-tolerant technology, which is applied in the field of microbial agent and its preparation, can solve the problems of poor product maturity, long fermentation cycle, and low degradation rate of food waste oil, so as to reduce oil content and purchase cost effect

Active Publication Date: 2018-12-18
苏州微宏生物科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a microbial agent with high salt resistance and oil degradation rate and its preparation method, which can solve the problems of high salt content in kitchen waste, low food waste oil degradation rate, long fermentation cycle, and product failure. The problem of poor maturity

Method used

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  • Microbial agent with high salt tolerance and high oil degradation rate, and preparation method thereof
  • Microbial agent with high salt tolerance and high oil degradation rate, and preparation method thereof
  • Microbial agent with high salt tolerance and high oil degradation rate, and preparation method thereof

Examples

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preparation example Construction

[0038] See figure 1 , the preparation method of the microbial bacterial agent with high salt resistance and oil degradation rate of the present invention, comprises the following steps:

[0039] S1. Provide raw materials and culture media for strains. Raw materials for strains include Bacillus phlei, Bacillus amyloliquefaciens, plant lactic acid bacteria, Saccharomyces cerevisiae, Bacillus megaterium, Streptomyces albicans and Aspergillus oryzae; culture media include solid media, liquid Medium and fermentation medium, wherein, solid medium includes beef extract peptone agar medium, potato dextrose agar medium and lactic acid bacteria solid medium; liquid medium includes beef extract peptone liquid medium, potato dextrose liquid medium and lactic acid bacteria Bacterial liquid culture medium; fermentation medium is: molasses 3%, corn steep liquor powder 8%, magnesium sulfate 0.2%, sodium hydroxide 0.15%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.1%,...

Embodiment 1

[0049] S1. Slope culture: Under sterile conditions, inoculate Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus megaterium on beef extract peptone agar medium for cultivation, and inoculate plant lactic acid bacteria on lactic acid bacteria solid medium (MRS) For culturing, Saccharomyces cerevisiae, Streptomyces cerevisiae and Aspergillus oryzae are inoculated in Potato Dextrose Agar Medium (PDA) for cultivation, wherein plant lactic acid bacteria, Saccharomyces cerevisiae, Bacillus megaterium, Streptomyces cerevisiae and Aspergillus oryzae , Bacillus subtilis and Bacillus amyloliquefaciens were cultured at 30°C for 2 days;

[0050] S2. Primary seed culture: Under aseptic conditions, single colonies were picked and inoculated in 50mL / 250mL liquid medium from the strains cultivated in step S1. Aspergillus was cultured on a shaker at 160r / min for 2 days at 30°C, Bacillus subtilis and Bacillus amyloliquefaciens were cultured on a shaker at 150r / min for 2 days at 30°C, an...

Embodiment 2

[0056] The preparation method of this example is basically similar to that of Example 1, the difference is that the inoculum amount in step S3 is changed from 5% to 4%, and the high-salt and high-fat kitchen waste treatment microbial bacterial agent of this example is obtained. The bacterial composition is:

[0057]

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Abstract

The invention relates to a microbial agent with a high salt tolerance and a high oil degradation rate, and a preparation method thereof. The microbial agent is prepared by slant culture, first-stage seed culture, secondary seed culture and mixed fermentation culture. The microbial agent comprises Bacillus subtilis, Bacillus amyloliquefaciens, Plant Lactobacilli, Saccharomyces cerevisiae, Bacillusmegaterium, Streptomyces albogriseolus and Aspergillus oryzae. The microbial agent with the high salt tolerance and high oil degradation rate is obtained by compounding above six bacterium raw materials, so the microbial agent can effectively degrade the main components (cellulose, oil, starch and proteins) of kitchen wastes in order to fully ferment the kitchen wastes, thereby the problems of high salt content, low oil degradation rate and long fermentation cycle of the kitchen wastes and poor product maturity are solved.

Description

technical field [0001] The invention relates to a microbial agent with high salt resistance and oil degradation rate and a preparation method thereof. Background technique [0002] Food waste refers to food residues and wastes generated in activities such as households, catering services, and unit meals. Statistics show that the proportion of kitchen waste in my country's urban household waste ranges from 37% to 62%, and the annual production of kitchen waste in major cities in China is about 60 million tons. Taking Beijing as an example, in 2014, Beijing's kitchen waste generated The volume is about 2600 tons / day. Kitchen waste is divided into two types: kitchen waste and slops: kitchen waste is food residues generated during food processing; it mainly comes from residential areas, food wholesale and retail markets, and various waste stations. Slop waste is the food residue after eating and drinking; it mainly comes from the catering industry, hotels and canteens of enterp...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14C05F11/08C05F9/04C12R1/125C12R1/07C12R1/25C12R1/865C12R1/11C12R1/465C12R1/69
CPCC12N1/14C12N1/18C12N1/20C05F9/04C05F11/08Y02A40/20
Inventor 丁国春李季王博
Owner 苏州微宏生物科技有限公司
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