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A kind of composite antibacterial agent and its application method

A compound antibacterial agent and dosage technology, which is applied in the direction of fungicides, chemical instruments and methods, botany equipment and methods, etc., can solve the problems of rapid spread, rapid loss of agents, and difficult detection of diseases, etc., to achieve enhanced agent Absorb, reduce chemical loss, prevent soil crusting effect

Active Publication Date: 2020-06-30
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, systemic Fusarium wilt spreads quickly, the disease is difficult to detect, and recovery is difficult. The existing medicaments are not effective in the prevention and treatment, and the medicaments are lost quickly and the efficacy time is short.

Method used

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  • A kind of composite antibacterial agent and its application method
  • A kind of composite antibacterial agent and its application method
  • A kind of composite antibacterial agent and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Pathogen Screening and Identification of Fusarium Wilt of Acer indicum

[0050] The diseased plants were collected and brought back to the laboratory for isolation of pathogenic bacteria. The different parts of the affected plants of Acer indica (root, phloem, xylem, and discolored vascular tissue with typical symptoms) were subjected to tissue separation and dilution coating methods. Bacteria were picked with an inoculation loop to pick a single colony for streaking purification, and fungi were picked with an inoculation loop. Needle-picked mycelia for purification.

[0051] A total of 91 bacterial strains were obtained through tissue separation and dilution coating, including 30 fungal strains and 61 bacterial strains. According to the colony morphology and microscopic observation, 15 strains with the highest frequency were used for subsequent inoculation experiments, and At-13 was isolated from the main xylem of all diseased plants by tissue isolation. The discolor...

Embodiment 2

[0056] Antagonism test of common V.dahliae control agents

[0057] Among them, common V.dahliae control agents are shown in Table 1

[0058] Table 1 Common V.dahliae control agents

[0059]

[0060] The above agents were configured into a poisonous plate with a final concentration of 0.005-1000 μg / mL, inoculated with mycelium blocks of pathogenic bacteria, and coated with about 120 spores / 50 μL of spore liquid. EC of the drug 50 .

[0061] The data analysis results and virulence regression equations of the growth inhibition of pathogenic bacteria by different concentrations of 7 kinds of medicaments are shown in Table 2 and Table 3. The bacteriostatic agents with better antibacterial effects are Shenzimycin and Benomyl. Strong inhibitory ability, EC 50 They were 0.3281 and 0.4966 μg / mL respectively; Ningnanmycin and chlorothalonil had poor inhibitory effects on At-13.

[0062] The inhibitory rate of table 2 fungicides to the growth of At-13 mycelium

[0063]

[006...

Embodiment 3

[0078] A compound antibacterial agent, the composite antibacterial agent includes solid medicament and liquid medicament; wherein the solid medicament contains polyacrylamide of 0.5g / kg, and the rest is nutrient soil; wherein the liquid medicament contains 1% Shenzimycin of 1ml / kg; 1ml / kg of silicone, the rest is sterile water.

[0079] 1% Shenzimycin drug from Shanghai Nongle was used to control 3-year-old maple ingot blight in indoor potted plants, with 10 trees in each group. Among them, the 3-year-old Acer ingot blight disease plants such as Figure 4 shown.

[0080] The branches and leaves of the Acer involucrata diseased plants in the Acer indica blight caused by V.dahliae are re-cut; the consumption of solid medicament is 0.2kg; the consumption of its liquid medicament is 3kg, wherein, the polyacrylamide of 0.1g is mixed with Mix 199.9g of sterilized nutrient soil, and sprinkle it on the roots of potted plants that have already been infected; then take 3ml of 1% Shen...

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Abstract

The invention discloses a compound antibacterial agent and an application method thereof. The compound antibacterial agent is prepared from a solid medicament and a liquid medicament, wherein the solid medicament is prepared from 0.1 to 1g / kg of polyacrylamide and the balance of nutrient soil; the liquid medicament is prepared from 0.5 to 1.5mg / kg of 1 percent Shenqinmycin, 0.25 to 1.5ml / kg of organic silicon and the balance of sterile water. The compound antibacterial agent disclosed by the invention aims at acer truncatum wilt caused by soil-borne pathogen Verticillium dahliae; a biogenic medicament Shenqinmycin is combined with the organic silicon and the polyacrylamide, and the components are compounded and proportioned; the internal absorption effect is much better than that of othermedicaments; the compound antibacterial agent can be used for killing pathogens colonized at roots and vascular tissues and effectively controlling disease conditions; moisture and medicament loss canbe reduced through water-retention and synergistic components, and the number of times of applying and manpower investment are reduced; the compound antibacterial agent is especially applicable to water-lacking drought regions.

Description

technical field [0001] The invention relates to the technical field of agricultural fungicide preparations, in particular to a compound antibacterial agent and its application method. Background technique [0002] The pathogen of Fusarium wilt of maple ingot has been screened and identified as Verticillium dahliae by Chen Fengmao's research group of Nanjing Forestry University. The relevant results have been published in the international journal of plant pathology "Plant Disease". The control method has not yet been overcome. Here Under the premise of this technical research. The characteristics and regularity of the disease, the targets of the pathogen, the cycle of the disease and the known control methods are briefly described as follows. [0003] Disease characteristics and regularity: Acer ingot blight occurs in low-lying farmland prone to water accumulation, interplanting with crops; initially, diseased trees are randomly distributed, and more diseased trees occur in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/60A01N55/10A01P3/00C09K17/20C09K101/00
CPCA01N43/60A01N55/00C09K17/20
Inventor 李欢陈凤毛李岱龙赵祥慧王文文程娟
Owner NANJING FORESTRY UNIV
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