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Marine acid producing strain for liquor making and application of marine acid producing strain

A technology of clostridia and liquor, which is applied in the preparation of bacteria, alcoholic beverages, microorganisms, etc., can solve the problems of poor temperature and dissolved oxygen tolerance, affect the aroma of liquor, and single source, achieve stable acid production performance, shorten preparation time, The effect of strong acid production ability

Active Publication Date: 2018-12-21
QINGDAO LANGYATAI GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the caproic acid bacterial agents used by liquor companies come from the screening and enrichment of the old cellar mud itself, and the source is relatively single. Poor tolerance to temperature, acidity and dissolved oxygen, low acid production, affecting the main aroma of liquor

Method used

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  • Marine acid producing strain for liquor making and application of marine acid producing strain
  • Marine acid producing strain for liquor making and application of marine acid producing strain
  • Marine acid producing strain for liquor making and application of marine acid producing strain

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: Screening of marine hexanoic acid bacterial strain

[0021] Collect marine sediment samples from areas such as the Yellow Sea and the Mariana Trench, put the collected samples into sterile sample bags, and store them at -80°C.

[0022] Take a sample of marine sediment and add it to sterile water, mix it evenly to obtain a suspension; inoculate the suspension into 50ml of RCM liquid medium, and enrich for 20 days at 30°C with anaerobic culture. The enriched culture solution was heat-shocked at 80°C for 15 minutes to kill the microbial vegetative body, enrich the spores, continue to inoculate into 50ml of RCM liquid medium, and enrich in anaerobic culture at 30°C for 15 days. After three consecutive enrichments, serial dilutions were carried out, and 10 -4 —10 -7 100 μl of the diluted suspension was spread on the RCM medium plate, and two plates were spread for each dilution. The inoculated plate was placed in a sealed anaerobic bag and incubated at 30°C ...

Embodiment 2

[0036] Embodiment 2: Preparation of artificial pit mud

[0037] 1. The strain Clostridium sp.SB2 was inoculated and fermented in a 100L fermenter in an enhanced Clostridium medium (RCM). The medium formula used was peptone 10g / L, beef extract 10g / L, yeast extract 3g / L, and glucose 5g / L, soluble starch 1g / L, sodium chloride 5g / L, sodium acetate 3g / L, cysteine ​​hydrochloride 0.5g / L, sterilized at 115°C for 30min. The liquid volume of the fermentation tank is 60L, the inoculation amount is 15%, the temperature is 30-35° C., and the anaerobic fermentation is carried out for 12 days to obtain the marine caproic acid bacteria fermentation liquid.

[0038] 2. Take 700kg of pit mud from the wall of the normal fermentation cellar and 350kg of pit mud from the bottom of the pool, and mix them evenly to make mixed pit mud. Take 60kg of yellow water, 60kg of marine caproic acid bacteria liquid, and 20kg of mixed pit mud to make a suspension.

[0039] 3. Mix 200kg of mixed pit mud and 1...

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Abstract

The invention provides a marine acid producing strain for liquor making and an application of the marine acid producing strain. The microorganism is a clostridium sp. SB2 strain with the preservationnumber being CCTCC M 2018621. The provided strain has high acid producing capacity, grows vigorously, is high in environmental suitability and stable in acid producing performance in a passage process, and pit mud is not prone to aging. Marine caproic acid bacteria are taken as the main microbial agent to be added to artificial pit mud, so that initial microbial content is increased. In a pit mudpreparation process, alcohol, sodium acetate, distillers' grains, glucoamylase and dry yeast are added for supporting besides yellow water and Daqu powder, nutrition sources are added, and preparationtime is shortened.

Description

technical field [0001] The invention belongs to the technical field of strain screening for liquor brewing, and in particular relates to a marine acid-producing strain for brewing and its application. Background technique [0002] In the traditional brewing process of liquor, there has always been a saying that "good wine comes from old cellars". Microbes, various organic acids and ethanol produced by their metabolism can produce fatty acid esters, which become the main flavor components of liquor. The ester content in high-quality liquor can reach about 0.6%, mainly ethyl acetate, ethyl caproate, ethyl lactate, etc. The acid-producing ability of microorganisms in the pit mud determines the ester-producing ability, which in turn affects the quality of liquor. With the expansion of the liquor industry, the number of pits is also increasing, but the preparation method of high-quality pit mud in the pits is relatively backward, and the quality of the original wine obtained is...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12G3/02C12R1/145
CPCC12G3/02C12N1/205C12R2001/145
Inventor 李悦明王泽欣徐建春晁进福
Owner QINGDAO LANGYATAI GRP
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