A kind of activator of cotton immune system and its application
A cotton and cotton plant technology, applied in the field of cotton immune system activator, to achieve the effect of improving resistance
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Embodiment 1
[0023] Example 1 Cotton Seedling Cultivation and Indole Treatment
[0024] Accelerate the germination of cotton seeds until the radicle grows to about 1-2cm, and place them in the hydroponic nutrient solution. The formula of the hydroponic solution is (in 2L volume): 5ml 1M KNO 3 ; 5ml 1M Ca(NO 3 )2.4H 2 0; 3ml Fe salt mother liquor (Fe salt mother liquor formula is: Na 2 .EDTA, 37.3mg / L; Fe(SO4) 2 .7H 2 O, 28.7mg / L); 3ml trace element mother liquor (the formula of trace mother liquor is: KI, 0.83mg / L; H 3 BO 3 , 6.2mg / L; MnS O4.4H 2 O, 22.3mg / L; ZnSO 4 .7H 2 O, 8.6 mg / L; Na 2 MoO 4 .2H 2 O, 0.25mg / L; CuSO 4 .5H 2 O, 0.025mg / L; CoCl 2 .6H 2 O, 0.025mg / L); 2ml 1M MgSO 4 .7H 2 O; 1ml 1M KH 2 PO 4 ; 1ml 1M KOH. The nutrient solution was changed every 4 days, and the cotton seedlings were cultivated in a light culture room at a temperature of 28°C, with a light of 120 μEM-2S-1, 16 hours of light and 8 hours of darkness, until the cotton plant grew to two leaves...
Embodiment 2
[0026] Example 2 Inoculation identification after indole treatment
[0027] After 24 hours of indole solution treatment, the conventional Verticillium dahliae V991 was inoculated with a spore concentration of 10 5 cells / ml, inoculated by root dipping method, and the inoculation time was 1min. After inoculation, the cotton seedlings were transferred to sterile nutrient soil, and the indole solution of corresponding concentration was irrigated once. On the 12th day after inoculation, the incidence of Verticillium wilt of cotton seedlings was counted, and the method was based on the method reported by Xu et al. (Xu et al., 2012). 16 days after inoculation with Verticillium dahliae, the wilting, yellowing and shedding of the plants in the indole treatment group were significantly reduced compared with the control treatment, especially the cotton plants treated with 500 μM and 1 mM indole could significantly enhance the resistance of cotton plants to Verticillium dahliae. sex (se...
Embodiment 3
[0028] Example 3 Transcriptome analysis after indole treatment
[0029] A. Indole Treatment Transcriptome
[0030] With the method described in Example 1, cotton seedlings were treated with 500 μM indole solution for 1h, 3h, 6h, 12h, 20h, and after 24h, the root tissue was taken to extract total RNA. The total RNA extraction method refers to the improved isothiocyanate of Zhu et al. Guanidine (Zhu et al., 2005). In the experiment, the water treatment group was used as the control, and a total of 3 biological repetitions were set up. Cotton root RNA samples treated with indole solution for 24 hours and control sample RNA were sent to Main Company (Beijing) for whole-genome transcriptome sequencing analysis. The results of transcriptome analysis showed that, compared with the control samples, indole treatment significantly activated the expression of genes related to disease resistance in the cotton immune system. Significant changes occurred in the gene expression of cotton ...
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