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Method for extracting rubusoside from sweet tea leaves

A technology of sweet tea leaves and rubusoside, which is applied in the field of extracting rubusoside, and can solve the problems of large amount of organic solvent, complicated operation and complex extraction process, etc.

Inactive Publication Date: 2019-01-11
湖北志林农业科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of this method is: the method of water extraction and alcohol precipitation can remove some impurities, such as protein, etc., but it cannot remove pigment, and the cost is high, and the cost of using polyamide resin for decolorization is also high
But, the shortcoming of this method is: the amount of organic solvent used by silica gel column chromatography is large, the operation is complicated, and industrialized large-scale production is difficult
However, the disadvantages of this method are: the extraction process is complicated, the requirements for equipment and operation are high, and the recovery rate is low.
But, the shortcoming of this method is: the gained sweet tea crude extract after macroporous adsorption separation is not purified through decolorization separation, and product crystal color is darker, and yield is lower, and quality stability is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

reference example 1

[0077] The acidic alumina used in the examples of the present invention is activated before use: first activate the acidic alumina at a high temperature of 550°C for 18 hours, and then wash it with an ethanol solution with a volume fraction of 95% until the effluent is colorless Until it is transparent and has no other smell except ethanol, it is finally washed with water until there is no ethanol smell.

Embodiment 1

[0079] (1) Crush and extract: Crush 5t of sweet tea leaves, pass through a 20-mesh sieve, add 50t of water, conduct continuous countercurrent extraction at 85°C for 1 hour, and filter with a 100-mesh filter cloth to obtain 35t of extract;

[0080] (2) Enzymolysis and flocculation: Add 35kg of cellulase preparation and 35kg of protease preparation to the 35t extract obtained in step (1), and carry out enzymolysis for 1 hour at 50°C with a pH value of 5. , under normal pressure, inactivated for 60s, plate cooling to room temperature, then FeCl3 was added to the enzymolysis solution for flocculation, stirring continuously until the pH value was 3, then stirring for 0.8h, standing for 1.2h, using saturated Adjust the pH value to 8 with lime water, then stir for 1.2 hours, let it stand for 1 hour, perform plate and frame pressure filtration with a plate filter membrane with a filter membrane pore size of 2.0 μm, and then wash the filter residue with 6 tons of water until it has no s...

Embodiment 2

[0088] (1) Crush and extract: Crush 5t of sweet tea leaves, pass through a 30-mesh sieve, add 100t of water, conduct continuous countercurrent extraction at 95°C for 3 hours, and filter with a 300-mesh filter cloth to obtain 95t of extract;

[0089] (2) Enzymolysis and flocculation: Add 47.5kg of hemicellulase preparation and 47.5kg of pectinase preparation to the 95t extract obtained in step (1), carry out enzymolysis at 55°C and pH value of 4 for 2 hours, and enzymatically The hydrolyzate was inactivated at 100°C under normal pressure for 40 seconds, cooled to room temperature by plate cooling, then FeSO4 was added to the enzymatic hydrolyzate for flocculation, stirring continuously until the pH value was 5, then stirred for 1.2 hours, and left to stand 0.8h, use saturated lime water to adjust the pH value to 9, then stir for 0.8h, let it stand for 3h, use a plate filter with a filter membrane pore size of 1.0μm for plate and frame pressure filtration, and then use 8t of wate...

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Abstract

The invention relates to a method for extracting rubusoside from sweet tea leaves. The method comprises the following steps: (1) crushing sweet tea leaves, sieving the material, adding water, continuously performing countercurrent extraction, and filtering the material; (2) adding an enzyme preparation for enzymatic hydrolysis, and performing steps of inactivation, cooling, flocculation, filtration, and filter residue washing with water; (3) performing ultrafiltration and nanofiltration; (4) performing adsorption on macroporous adsorption resin chromatography column, washing the material withwater, discarding a washing solution, performing gradient elution with an organic solvent, and performing vacuum concentration; (5) performing adsorption on an alumina column, washing the material with water, and performing vacuum concentration; (6) adding activated carbon, stirring the material, filtering the material, and performing vacuum concentration and spray drying; and 7) dissolving the material with the organic solvent, and performing steps of filtering, crystallization, pumping filtration, vacuum drying, and crushing to obtain the rubusoside product. The extracted rubusoside productpresents pure white color, the purity is greater than or equal to 99%, and the yield is greater than or equal to 90.5%. The method of the invention has the advantages of simple operation process, short extraction time, low energy consumption and low cost, and can realize continuous large-scale production.

Description

technical field [0001] The invention relates to a method for extracting rubusoside, in particular to a method for extracting rubusoside from sweet tea leaves. Background technique [0002] Sweet tea is a perennial deciduous shrub belonging to Rubus of Rosaceae. It is mainly produced in the Dayao area of ​​Guangxi. The main medicinal part is sweet tea. [0003] Rubus is a natural sweetener with high sweetness and low calorie. Its sweetness is about 300 times that of sucrose, but its calories are only 1% of sucrose. Therefore, it is suitable for patients with diabetes, obesity and hyperlipidemia. Widely used in food, beverages, etc., has a huge market value. However, since sweet tea leaves contain a large amount of sweet tea polyphenols and flavonoids, it is difficult to completely remove them during the separation and purification process, which will affect the quality of rubusoside extract, mainly manifested in the bitter taste and darker color of rubusoside extract . [...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
CPCC07H15/256C07H1/08
Inventor 肖玉林
Owner 湖北志林农业科技开发有限公司
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