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A method for extracting rubusoside from sweet tea leaves

A technology for sweet tea leaves and sweet tea glycosides, applied in the field of extracting sweet tea glycosides, can solve the problems of low recovery rate, large amount of organic solvent, high equipment and operation requirements

Active Publication Date: 2020-06-26
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of this method is: the method of water extraction and alcohol precipitation can remove some impurities, such as protein, etc., but it cannot remove pigment, and the cost is high, and the cost of using polyamide resin for decolorization is also high
But, the shortcoming of this method is: the amount of organic solvent used by silica gel column chromatography is large, the operation is complicated, and industrialized large-scale production is difficult
However, the disadvantages of this method are: the extraction process is complicated, the requirements for equipment and operation are high, and the recovery rate is low.
But, the shortcoming of this method is: the gained sweet tea crude extract after macroporous adsorption separation is not purified through decolorization separation, and product crystal color is darker, and yield is lower, and quality stability is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

reference example 1

[0077] The acidic alumina used in the examples of the present invention is activated before use: first activate the acidic alumina at a high temperature of 550°C for 18 hours, and then wash it with an ethanol solution with a volume fraction of 95% until the effluent is colorless Until it is transparent and has no other smell except ethanol, it is finally washed with water until there is no ethanol smell.

Embodiment 1

[0079] (1) Crush and extract: Crush 5t of sweet tea leaves, pass through a 20-mesh sieve, add 50t of water, conduct continuous countercurrent extraction at 85°C for 1 hour, and filter with a 100-mesh filter cloth to obtain 35t of extract;

[0080] (2) Enzymolysis and flocculation: Add 35kg of cellulase preparation and 35kg of protease preparation to the 35t extract obtained in step (1), and carry out enzymolysis for 1 hour at 50°C with a pH value of 5. , under normal pressure, inactivate for 60s, plate cooling to room temperature, and then FeCl 3 Add it to the enzymatic hydrolysis solution for flocculation, stir continuously during the addition until the pH value is 3, then stir for 0.8h, let stand for 1.2h, adjust the pH value to 8 with saturated lime water, stir for 1.2h, let stand for 1h, Use a plate-type filter membrane with a filter membrane pore size of 2.0 μm for plate-and-frame press filtration, then wash the filter residue with 6 t of water until it has no sweet taste...

Embodiment 2

[0088] (1) Crush and extract: Crush 5t of sweet tea leaves, pass through a 30-mesh sieve, add 100t of water, conduct continuous countercurrent extraction at 95°C for 3 hours, and filter with a 300-mesh filter cloth to obtain 95t of extract;

[0089] (2) Enzymolysis and flocculation: Add 47.5kg of hemicellulase preparation and 47.5kg of pectinase preparation to the 95t extract obtained in step (1), carry out enzymolysis at 55°C and pH value of 4 for 2 hours, and enzymatically The solution was inactivated at 100°C under normal pressure for 40 seconds, cooled to room temperature by plate type, and FeSO 4 Add it to the enzymatic hydrolysis solution for flocculation, stir continuously during the addition until the pH value is 5, then stir for 1.2h, let it stand for 0.8h, adjust the pH value to 9 with saturated lime water, stir for another 0.8h, let it stand for 3h, Use a plate filter with a filter membrane pore size of 1.0 μm for plate and frame press filtration, then wash the filt...

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Abstract

The invention relates to a method for extracting rubusoside from sweet tea leaves. The method comprises the following steps of (1) smashing and sieving the sweet tea leaves, adding water and conducting continuous countercurrent extraction and filtration; (2) adding an enzyme preparation for enzymolysis, conducting inactivation, cooling, flocculation and filtration and washing filtered residues with water; (3) conducting ultrafiltration and nanofiltration; (4) conducting adsorption through a macroporous adsorption resin chromatographic column, conducting washing with water, discarding water washing liquid and conducting gradient elution with an organic solvent and concentration under reduced pressure; (5) conducting adsorption through an aluminum oxide chromatographic column and conductingwashing with water concentration under reduced pressure; (6) adding activated carbon and conducting stirring, filtration, concentration under reduced pressure and spray drying; (7) dissolving the organic solvent and conducting filtration, crystallization, suction filtration, vacuum drying and pulverization to obtain a rubusoside product. The rubusoside product extracted by the method has pure white color, the purity is greater than or equal to 99%, and the yield is greater than or equal to 90.5%. The method has a simple operation process, short extraction time, low energy consumption and a lowcost and can achieve continuous large-scale production.

Description

technical field [0001] The invention relates to a method for extracting rubusoside, in particular to a method for extracting rubusoside from sweet tea leaves. Background technique [0002] Sweet tea is a perennial deciduous shrub belonging to Rubus of Rosaceae. It is mainly produced in the Dayao area of ​​Guangxi. The main medicinal part is sweet tea. [0003] Rubus is a natural sweetener with high sweetness and low calorie. Its sweetness is about 300 times that of sucrose, but its calories are only 1% of sucrose. Therefore, it is suitable for patients with diabetes, obesity and hyperlipidemia. Widely used in food, beverages, etc., has a huge market value. However, since sweet tea leaves contain a large amount of sweet tea polyphenols and flavonoids, it is difficult to completely remove them during the separation and purification process, which will affect the quality of rubusoside extract, mainly manifested in the bitter taste and darker color of rubusoside extract . [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/256C07H1/08
CPCC07H1/08C07H15/256
Inventor 陈钱黄华学贺进军
Owner HUNAN HUACHENG BIOTECH
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