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Composite biological microbial agent for preventing and treating SRB under wide temperature environment and application method thereof

A microbial strain and microbial technology, applied in the direction of microbial-based methods, biochemical equipment and methods, and drilling compositions, can solve problems such as localized corrosion of treatment objects, high-temperature corrosion at the bottom of oil wells, etc., and achieve control of content, The effect of strong applicability, improving ability and scope of application

Active Publication Date: 2019-01-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that most of the current biological control SRB methods can only solve the problem of local corrosion of the treatment object, such as the anti-corrosion problem of the sewage station at 30-50 ℃, and the high-temperature corrosion problem at the bottom of the oil well, which cannot be used for the whole process and the whole temperature section at one time To achieve system anticorrosion, the present invention proposes a composite biological agent for preventing and treating SRB in a wide temperature environment and its application method

Method used

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  • Composite biological microbial agent for preventing and treating SRB under wide temperature environment and application method thereof
  • Composite biological microbial agent for preventing and treating SRB under wide temperature environment and application method thereof
  • Composite biological microbial agent for preventing and treating SRB under wide temperature environment and application method thereof

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Experimental program
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Effect test

Embodiment 1

[0019] JSHD-3 bacterium of the present invention is screened by following process:

[0020] (1) Sample collection

[0021] The produced fluids of 6 oil wells were collected in Jiangsu Oilfield. The names of the samples are: CH2-9, CH2-48, T83-8, H88-7, H88-39, and Zhen1-1.

[0022] (2) Selective medium for denitrifying bacteria

[0023] Potassium nitrate 0.2%, glucose 0.1%, magnesium chloride 0.01%, calcium chloride 0.01%, potassium dihydrogen phosphate 0.005%, add water to make up to 100%, adjust pH to 7.0-7.2.

[0024] Solid medium: 2% agar is added to the liquid medium.

[0025] (3) Enrichment culture

[0026] Mix the output fluid samples of 6 oil wells in step (1) in equal proportions, add nitrate-reducing bacteria enrichment and isolation medium components to the output fluid of Jiangsu Oilfield, and then culture at 65°C for 7 days to obtain enrichment culture sample. Using the above-mentioned enriched sample as the initial inoculum, the nitrate-reducing bacteria enr...

Embodiment 2

[0059] CPB-015 bacterium of the present invention is screened by following process:

[0060] (1) Sample collection

[0061] Water samples and soil samples were collected from different oil wells in Jiangsu Oilfield. Among them, there are 9 water samples in total, numbered Wei 2 Sanfen, Ying 4 Sanfen, H88-1, H88-10, H88-31, Wei 2-87, Wei 15-17, Wei 2-53, Wei 11 -1. 4 soil samples, numbered Wei 2-57, Huang 58-29, Huang 88-3, Wei 11-1.

[0062] (2) Enrichment culture

[0063] Take 100mL of water samples from different samples, and use high-speed centrifugation (12000rpm, 15min) to concentrate the microorganisms in the water samples. Remove the supernatant, suspend the precipitate with 2 mL of sterile water, and take 1 mL of the suspension in a 70°C water bath for 30 minutes to screen for denitrifying bacteria that are tolerant to high temperatures. Add the high-temperature-treated and untreated suspensions into the pre-sterilized denitrifying bacteria selective liquid medium...

Embodiment 3

[0072] Insert 0.5mL CPB-015+0.5mL JSHD-3 shake flask spore suspension into 25mL enrichment medium respectively (spore concentration: 10 8 cfu / mL), cultured at different temperatures of 40°C and 60°C, and observed the growth and reproduction of the two denitrifying bacteria under the common nutrient system.

[0073] After the mixed inoculation was cultured at 40°C for 16 hours, the CPB-015 strain obviously multiplied and grew, while JSHD-3 did not grow. Microscopic examination observed the CPB-015 bacteria in the logarithmic growth phase, and the OD value of the bacteria concentration was 0.459, which was not significantly different from the OD value of 0.471 when CPB-015 was inoculated alone for 16 hours. Cultivate to 24h, bacterium concentration reaches maximum value, and OD value is 0.539, and contrast OD600 is 0.542 ( figure 2 ). On the contrary, after culturing at 60°C for 16 hours, the CPB-015 strain did not grow and reproduce, while JSHD-3 obviously reproduced and gre...

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Abstract

The invention discloses a composite biological microbial agent for preventing and treating SRB under wide temperature environment and an application method thereof, belonging to the technical field ofmicrobial prevention and control of sulfate reducing bacteria. A wide-temperature composite biological microbial agent comprises a medium-low temperature denitrifying bacterium CPB-015, medium-high temperature denitrifying bacteria JSHD-3, sodium nitrate and sodium dihydrogen phosphate. The invention can effectively stimulate the growth of denitrifying bacteria in an isothermal environment of anoil well, thereby inhibiting the rapid growth of SRB under different temperature environments, controlling the content of sulfide, and improving water quality. This kind of denitrifying compound microbial agent with strong applicability and wide inhibition range can not only effectively improve the desulfurization and anticorrosion ability of denitrifying bacteria, but also has important practicalsignificance to promote the industrial application of denitrifying bacteria.

Description

technical field [0001] The invention relates to the technical field of microbial prevention and control of sulfate-reducing bacteria, in particular to a composite biological agent for preventing and treating SRB under a wide temperature environment and a method of use thereof. Background technique [0002] The development of reservoir water injection leads to the growth of sulfate reducing bacteria (SRB) in reservoirs, oil pipelines and oil production units, resulting in acidification of reservoirs and corrosion of pipelines, which has brought serious losses to the global petroleum industry. With the successful application of denitrifying bacteria (DNB) technology to inhibit sulfate reducing bacteria (SRB) in foreign oilfields, more and more attention has been paid to the research on biological control technology of using microorganisms to inhibit or eliminate microbial corrosion in domestic oilfields. However, the main research object of this technology is SRB treatment of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K8/54E21B41/02C12R1/11C12R1/01
CPCC09K8/54C09K2208/32C12N1/20C12N1/205C12R2001/01C12R2001/11E21B41/02
Inventor 吴伟林李凡磊袁玉峰杨帆姚峰康玉阳
Owner CHINA PETROLEUM & CHEM CORP
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