Efficient regulation and control composition for glycometabolism of aquatic products as well as preparation method and application of efficient regulation and control composition
A technology for sugar metabolism and composition, applied in the field of high-efficiency aquatic product sugar metabolism regulation composition and preparation thereof, can solve problems such as limited application, toxic and side residue, food safety, etc. The effect of utilization
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Embodiment 1
[0043] Mix β-mannanase, ferulic acid esterase and cellulase according to the mass ratio of 1:2:10 to make a mixed enzyme preparation; then uniformly mix with destarched wheat bran according to the mass ratio of 1:20, and seal Enzymatic hydrolysis in a water bath at 40°C for 24 hours; the enzymatic hydrolyzate was sterilized at 120°C and centrifuged at a speed of 5000r / min for 60min, and the supernatant was evaporated at a temperature of 110°C to obtain the enzymatic hydrolyzate of wheat bran. The mass content of mannan and the mass content of ferulic acid in the enzymatic hydrolysis product of wheat bran are greater than 35% and 28% respectively, as measured by liquid chromatography.
Embodiment 2
[0045] Mix β-mannanase, ferulic acid esterase and cellulase according to the mass ratio of 1:3:8 to make a mixed enzyme preparation; then uniformly mix with destarched wheat bran according to the mass ratio of 2:18, and seal Enzymolysis in a water bath at 35°C for 24 hours; the enzymolysis solution was sterilized at 120°C and centrifuged at a speed of 5000r / min for 30 minutes, and the supernatant was evaporated at a temperature of 100°C to obtain the enzymatic hydrolysis product of wheat bran. The mass content of mannan in the enzymatic hydrolysis product of wheat bran is greater than 38%, and the mass content of ferulic acid is greater than 30% as measured by liquid chromatography.
Embodiment 3
[0047] Mix β-mannanase, ferulic acid esterase and cellulase according to the mass ratio of 1:5:6 to make a mixed enzyme preparation; then uniformly mix with destarched wheat bran according to the mass ratio of 2:15, and seal Enzymolysis in a water bath at 40°C for 12 hours; the enzymolysis solution was sterilized at 120°C and then centrifuged at a speed of 3000r / min for 60 minutes, and the supernatant was rotary evaporated at a temperature of 100°C to obtain the enzymatic hydrolysis product of wheat bran. The mass content of mannan and the mass content of ferulic acid in the enzymatic hydrolyzate of wheat bran are greater than 42% and 33% respectively, as measured by liquid chromatography.
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