A microbial fermentation product obtained by culturing in a musical environment, and a preparation method and use thereof

A technology of microbial fermentation and environment, applied in the field of medicine or preparation of medicines to protect the liver, can solve the problems of excretion, low bioavailability, accumulation of toxic side effects, etc.

Pending Publication Date: 2019-01-25
百岳特生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the modern age when people strive to pursue good health, various nutritional and weight-loss supplements gradually appear on the market to meet people's needs; however, most of these nutritional and weight-loss supplements are chemically synthesized or derived from ingredients that do not exist in natural foods. Combination of a single molecular structure, the source of raw materials are petroleum extracts, coal asphalt derivatives, chemically processed sugar or processed industrial products, but these chemically refined nutrients are difficult to be recognized by cells , Accept and utilize, so more nutrients will be actively excreted with urine or sweat
Due to the low absorption rate, low residence time in the body, and low bioavailability of these nutritional supplements, the chemically synthesized nutrients have not achieved the declared efficacy, and the content may be hundreds or even thousands of times the daily recommended intake. High doses will have It may bring the risk of toxic reactions to the human body; although the body will actively excrete excess nutrients, it also indicates that the unabsorbed chemical nutrients need liver and kidney metabolism, which will increase the extra burden on the human body; and long-term consumption of chemically synthesized nutrients may cause Cumulative toxicity and side effects, even risk of cancer

Method used

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  • A microbial fermentation product obtained by culturing in a musical environment, and a preparation method and use thereof
  • A microbial fermentation product obtained by culturing in a musical environment, and a preparation method and use thereof
  • A microbial fermentation product obtained by culturing in a musical environment, and a preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The method for the microbial fermented product cultivated under the music environment of embodiment 1 of the present invention

[0038] In a preferred embodiment of the present invention, vegetable juice (freshly squeezed juice) and berry juice (freshly squeezed juice) are mixed in a ratio of 3 to 2, and then diluted 5 times with distilled water as the empty culture solution; Juices containing: blueberry, cranberry, red grape, white grape, bayberry, mulberry, apple, carrot, sugar cane, passion fruit, pineapple, and lemon in equal parts; vegetable juice containing: broccoli, celery, and asparagus, their mixture in equal proportions. The air-culture liquid was treated by Pasteur sterilization, and then Streptococcus thermophilus (Streptococcus thermophilus), Saccharomyces cerevisiae (Saccharomycescerevisiae) and acetic acid bacteria (Acetobacter aceti) were introduced into the air-culture liquid for pre-fermentation. Ferment at 30°C ± 1°C for 192 hours at a constant temp...

Embodiment 2

[0039] Example 2 Detection of the superoxide dismutase (SOD-like) content of the microbial fermentation product of the present invention

[0040] Superoxide dismutase (SOD) is an enzyme that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide. These enzymes play an important role in antioxidant defense in cells exposed to oxygen. Therefore, the present invention utilizes SOD activity kit (SOD activity kit, Enzo Life Science Company, model CAT#ADI-900-157) to analyze cellular oxidative stress.

[0041] The result is as figure 1 As shown, compared with the control group (only the enzymes that did not listen to music), no matter in which kind of music environment (classical music, pop music and rock music), the microbial fermentation products cultivated can effectively improve the superoxide dismutase ( SOD-like) content is more than 1 times, it can be inferred that its antioxidant capacity will be higher than that of microbial fermentation product...

Embodiment 3

[0042] Embodiment 3 Stomach cell antioxidant capacity of the microbial fermentation product of the present invention

[0043] The present invention uses a cell platform to test the antioxidant capacity, and uses quantitative real-time polymerase chain reaction (Quantitative real time PCR, qPCR) to detect the superoxidation of AGS human gastric epithelial cells (gastric epithelial cells) treated with the microbial fermentation product of the present invention. The gene expression level of substance dismutase 2 (SOD2) was used to analyze the gastric cell antioxidant capacity of the microbial fermentation product of the present invention.

[0044] The present invention uses human gastric epithelial cells purchased from American Type Culture Collection (ATCC), number CRL-1739. The cells were cultured in a medium supplemented with 10% fetal bovine serum (FBS) (GIBCO Company, No. 10438-026, USA) and 1% penicillin / streptomycin (GIBCO Company, No. 15140-122, USA) RPMI 1640 basal medi...

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Abstract

The invention relates to a microbial fermentation product and a preparation method and use thereof. The microbial fermentation product is a mixed liquid of vegetable juice and raspberry juice which isobtained by fermentation of streptococcus thermophilus, yeast and acetic acid bacteria under the environment of playing music. The microbial fermentation product of the present invention has uses forpreparing pharmaceutical aspects that promote metabolism of adipocytes or for preparing pharmaceutical aspects that protect the liver.

Description

technical field [0001] The invention provides a microbial fermented product obtained by culturing in a music environment, a preparation method thereof, and an application for preparing a drug for promoting fat cell metabolism or preparing a drug for protecting the liver. Background technique [0002] Obesity has long been defined as a disease condition associated with most health problems as well as a reduced lifespan. In the modern age when people strive to pursue good health, various nutritional and weight-loss supplements gradually appear on the market to meet people's needs; however, most of these nutritional and weight-loss supplements are chemically synthesized or derived from ingredients that do not exist in natural foods. Combination of a single molecular structure, the source of raw materials are petroleum extracts, coal asphalt derivatives, chemically processed sugar or processed industrial products, but these chemically refined nutrients are difficult to be recogn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18A61K36/899A61P3/04A61P1/16C12R1/46C12R1/865C12R1/02
CPCA61K36/185A61K36/23A61K36/31A61K36/45A61K36/605A61K36/73A61K36/752A61K36/87A61K36/88A61K36/899A61K2236/19A61P1/16A61P3/04C12N1/18C12N1/20A61K2300/00
Inventor 林咏翔许栢峻何政育
Owner 百岳特生物科技(上海)有限公司
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