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A technology of volatile oil and male clove, applied in the direction of application, chemicals for biological control, fungicides, etc., to achieve the effect of being volatile, easy to degrade, and protecting the ecological environment
Inactive Publication Date: 2019-02-01
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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[0006] At present, there is no document disclosure related to the technical solution of the present invention
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Embodiment 1
[0022] Embodiment 1: the preparation of male clove volatile oil
[0023] (1) Add 8 times the mass of water to 1 kg of crushed male clove powder, soak for 2 hours, and use steam to extract the volatile oil in male cloves by means of heating and distillation. The extraction time is 6 hours, and the effluent is collected by condensation;
[0024] (2) The extracted volatile oil was dried with anhydrous sodium sulfate to remove the contained water, put into a brown sample bottle, sealed, and stored at -20°C until use.
Embodiment 2
[0025] Embodiment 2: the activation of pathogenic bacteria
[0026] (1) The pathogenic bacteria isolated from the roots and shoots of Panax notoginseng were purified and identified as Fusarium oxysporum ( Fusarium oxysporum ), Fusarium solani ( Fusarium solani ), Pythium ( Pythium aphanidermatum ), Cylindrosporium destroya ( Cylindrocarpon destructans ), Botrytis ( Botrytis cinerea ), Glosporum anthracnose ( Colletotrichum gloeosporioides ) and Rhizoctonia solani ( Rhizoctonia solani ) strains were inoculated on PDA medium for activation, and after inoculating 3 to 4 times, the vigorously growing strains were taken for use;
[0027] (2) Potato dextrose agar medium (PDA): 200g potato, 20g glucose, 20g agar, 1000mL distilled water.
Embodiment 3
[0028] Embodiment 3: GC-MS measures the chemical composition of male clove volatile oil
[0030] (2) EI source Ionization source temperature: 230°C; quadrupole temperature: 150°C; scanning range: 30-500m / z; inlet temperature: 285°C; split ratio 10:1; Energy: 70eV; temperature program of column oven: 50°C for 4min, 5°C / min to 120°C, 1°C / min to 180°C, 10°C / min to 280°C and hold for 16min; The retention time of the alkane continuous carbon (C9-C25) is calculated, and the retention time and mass spectrum of the compound are compared with the NIST 17.L database to determine the final compound. The test results are shown in the table below;
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Abstract
The invention discloses uses of clove bud volatile oil, particularly applications of clove bud volatile oil in prevention and control of root rot of Chinese medicinal materials or crops in agricultureor applications of clove bud volatile oil in prevention and control of pathogenic bacteria causing diseases in the aerial parts of Chinese medicinal materials or crops. According to the present invention, the experiment results show that the clove bud volatile oil can inhibit the mycelial growth of four main pathogenic bacteria causing Panax notoginseng root rot and comprising Fusarium oxysporum,Fusarium solani, Pythium aphanidermatum and Cylindrocarpon destructans, can completely inhibit the mycelial growth of three pathogenic bacteria causing the diseases in the aerial parts of Panax notoginseng and comprising Botrytis cinerea, Colletotrichum gloeosporioides and Rhizoctonia solani, and has the antibacterial effect equivalent to the antibacterial effect of the chemical pesticide hymexazol, wherein the compounding of clove bud and hymexazol can provide synergistic effect for a variety of strains; the prevention and control effect on Panax notoginseng blade gray mold by the clove budvolatile oil is up to 60.77%; and the volatile oil has characteristics of easy volatility and decontamination through aroma, such that the fertilizers or pesticides developed from the volatile oil have advantages of safety, effectiveness, low residue and the like.
Description
technical field [0001] The invention relates to the application of clove volatile oil, in particular to the control effect of male clove volatile oil on root rot of Chinese medicinal materials or crops and various diseases of Chinese medicinal materials or aboveground parts of crops, and belongs to the technical field of biopesticides. Background technique [0002] Panax notoginseng ( Panax notoginseng (Burk.) F.H.Chen), also known as Tianqi, is a perennial herb of Araliaceae ginseng. It has the functions of dispelling blood stasis and hemostasis, reducing swelling and relieving pain. It is a rare and precious cultivated medicinal plant unique to my country. , which has a cultivation history of more than 400 years in Wenshan Prefecture, Yunnan Province. The roots, stems, leaves, and flowers of Panax notoginseng can be used as medicine. The range of medicines is very wide, and the social demand is constantly increasing. With the rapid increase of the planting area of Panax...
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