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Fermentation method of corn straw, fermented corn straw prepared by method and applications of fermented corn straw

A technology of corn stalks and fermentation methods, which is applied to the fermentation of corn stalks, fermented corn stalks and its application fields. It can solve the problems of complex composition of natural compound strains, low fermentation efficiency of single strains, and uncertainty in the fermentation process. Explosion time, growth performance improvement, number reduction effect

Inactive Publication Date: 2019-02-01
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These natural organic polymers have a very firm structure, can only absorb water and swell, and cannot be decomposed by the digestive juices and enzymes of monogastric animals. They are only fermented by a small amount of cecal microorganisms, and the digestibility is very low. They are only suitable for ruminant livestock. feed
[0004] Using microbial fermentation technology to improve the feeding value of straw is a research hotspot in the animal husbandry and feed industries. However, in the prior art, the composition of natural compound strains is complex, the fermentation efficiency of a single strain is low, and the fermentation process of artificial compound strains is strictly controlled. The effect is interfered by many factors, and the fermentation process is uncertain

Method used

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  • Fermentation method of corn straw, fermented corn straw prepared by method and applications of fermented corn straw

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A fermentation enhancer, consisting of the following components in parts by weight:

[0046] Yeast culture 12 parts, tea tree essential oil 1.6 parts, grape seed extract 0.8 parts, mannitol 0.6 parts, glucose oxidase 0.6 parts, casein 0.6 parts, vitamin B 1 0.03 parts, trace elements 0.05 parts.

[0047] Described trace element is:

[0048] K H 2 PO 4 5.0g / L, K 2 HPO 4 2.0g / L, MgSO 4 2.0g / L, FeSO 4 2.8g / L, CuSO 4 3.0g / L, MnSO 4 1.5g / L, ZnSO 4 2.4g / L, CaCl 2 2.4g / L, water balance.

[0049] The yeast culture is formed by mixing liquid fermentation culture and solid fermentation culture in a weight ratio of 5:1.

[0050] The preparation method of yeast liquid fermentation culture:

[0051] The number of Candida utilis (Candida utilis) is AS2.281, Beina Chuanglian Biotechnology Co., Ltd.;

[0052] Activation medium: malt juice agar.

[0053]The liquid fermentation medium is: glucose 40g / L, peptone 10g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 0.3g / L, add ...

Embodiment 2

[0063] Activation of Aspergillus niger

[0064] Crush soybean shells and pass through a 10-mesh sieve, then sterilize at 121°C for 15 minutes; adjust the water content to 50-65%, and inoculate the Aspergillus niger strains activated by the bean juice slant medium into the bean shell medium at an inoculation amount of 1%. , cultured at 25-30°C for 48-60 hours to obtain fermentation strains with a content of ≥10 9 cfu·g -1 .

[0065] Bacillus subtilis ACCC 11025, China Agricultural Microorganism Culture Collection Management Center;

[0066] Activation and fermentation medium: peptone 0.5--3g, glucose 1--3g, KH 2 PO 4 0.02--0.08g, MgSO 4 0.01--0.04g, NaCl 0.2--0.4g, distilled water 100mL.

[0067] Inoculate the Bacillus subtilis ACCC 11025 strain on a solid plate for activation. The activated strain is inoculated in the liquid fermentation medium with an inoculum of 1%, and cultured at 37°C and 200rpm for 24-36h until the bacterial content is ≥10 9 cfu / mL.

[0068] The B...

Embodiment 3

[0074] A method for fermenting corn stalks, the steps are as follows:

[0075] (1) After the corn stalks are removed from impurities, mechanically pulverize them, pass through a 20-mesh sieve, adjust the moisture content of the corn stalks to 10%, steam blasting at 2.5 MPa for 50 seconds, and steam blasting at 2.0 MPa for 5 minutes;

[0076] (2) High-pressure steam sterilize the material after steam explosion at 121°C for 30 minutes, and cool to room temperature after the sterilization is completed;

[0077] (3) adjust the water content of the material after sterilization to 70-85%, and carry out aerobic fermentation after adding 1.5% of Bacillus subtilis, Bacillus amyloliquefaciens, the composite bacterial agent of Aspergillus niger and 3% fermentation enhancer therein, 28℃~32℃, ferment for 36 hours, then ferment for 24 hours at 32~35℃;

[0078] The weight ratio of Bacillus subtilis, Bacillus amyloliquefaciens and Aspergillus niger in the composite bacterial agent is 1:1:1; ...

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PUM

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Abstract

The invention discloses a fermentation method of corn straw, fermented corn straw prepared by the method and applications of the fermented corn straw, and belongs to the technical field of biologicalfermentation. The fermentation method of corn straw comprises the following processes: steam explosion, aerobic fermentation, and anaerobic fermentation. The method for fermenting corn straw of the invention can effectively degrade cellulose components in the corn straw, and increase the protein content of the corn straw. When feeding livestock and poultry, the fermented corn straw of the invention can significantly improve the growth performance of animals, maintain the intestinal environment stability and reduce the number of enteric pathogenic bacteria.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for fermenting corn stalks, fermented corn stalks prepared by the method and applications thereof. Background technique [0002] Crop straw is an auxiliary product of agricultural production, which has not been fully and effectively utilized in our country. Traditional planting habits usually return a large amount of crop straw directly to the field, discard it, or even burn it, which not only pollutes the environment and affects the construction of a harmonious society, but also causes the economic value of the straw to be lost. [0003] Crop straw has high crude fiber content, is difficult to be digested and absorbed by animals, has few available nutrients, and has poor palatability. It is classified as roughage in feed taxonomy. Cellulose, hemicellulose, and lignin are closely combined and intertwined to form crude fibers, also known as li...

Claims

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Application Information

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IPC IPC(8): A23K10/30A23K10/12A23K40/00
CPCA23K10/12A23K10/30A23K40/00A23V2400/125A23V2400/181A23V2400/173A23V2400/113Y02P60/87
Inventor 唐娟杨庆利于春娣朱英莲赵海燕
Owner QINGDAO AGRI UNIV
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