Thin-layer chromatographic scanner and analysis method by use of scanner

A thin-layer chromatography and analysis method technology, applied in the field of new thin-layer chromatography scanners and new chromatographic instruments, can solve the problems of cumbersome and time-consuming, and achieve the effect of simple and lightweight instruments

Inactive Publication Date: 2019-02-01
河北路科环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1971, the API260 project jointly funded by the U.S. Bureau of Mines (USBM) and the American Petroleum Institute (API) first proposed to divide heavy oil into five groups: acidic components, basic components, neutral nitrogen-containing compounds, and saturated oils. Hydrocarbons and aromatics, this method is called the USBM-API method, which is a circular chromatography method that can recycle the eluting solvent, but its disadvantage is that it is very cumbersome and time-consuming, and only the separation of acidic components and basic components Continuous cycle washing is required for 6 days, so this method is only used when conducting special research on the acidic or alkaline components of petroleum samples or detailed compositional studies on samples

Method used

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  • Thin-layer chromatographic scanner and analysis method by use of scanner
  • Thin-layer chromatographic scanner and analysis method by use of scanner
  • Thin-layer chromatographic scanner and analysis method by use of scanner

Examples

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Embodiment 1

[0019] Example 1: Determination of Petroleum Components in Soil Sample Soluble Organic Matter

[0020] The main instrument used in the determination is the device developed by the present invention, and the supporting devices include 1 centrifuge, 3 chromatographic cylinders, 1 constant humidity chamber, 1 electronic balance, a number of micro-injectors and reagents.

[0021] Experimental steps:

[0022] 1. Weigh 5g of soil sample, extract and centrifuge with chloroform, take 5-10mg of the supernatant into a sample bottle, add 500μL of dichloromethane with a micro-syringe, close the cap tightly and shake gently to fully dissolve the sample;

[0023] 2. Prepare the chromatographic solution, add n-hexane, toluene, methylene chloride:methanol 95:5 to the three chromatographic cylinders respectively, the height of the solvent liquid level in each cylinder is about 10mm, cover the cylinder lid and seal it for 30 minutes;

[0024] Three, sampling:

[0025] 1. Use a micro-syringe t...

Embodiment 2

[0051] Example 2: Determination of Petroleum Group Components in Crude Oil Samples

[0052] The main instrument used for the determination is the device developed by the present invention, and the supporting devices include 3 chromatographic cylinders, 1 constant humidity chamber, 1 electronic balance, a number of micro-injectors and reagents.

[0053] Experimental steps:

[0054] 1. Filter the crude oil sample into the sample vial with a 0.22 μm organic microporous membrane, then add 500 μL of dichloromethane with a micro syringe, close the cap tightly and shake gently to fully dissolve the sample;

[0055] 2. Prepare the chromatographic solution, add n-hexane, n-hexane:dichloromethane (1:1V:V), n-hexane:isoamyl alcohol (9:1V:V) into the three chromatography tanks respectively. The height of the solvent liquid level in each cylinder is about 10mm. Cover the cylinder lid and seal it for 30 minutes;

[0056] Three, sampling:

[0057] 1. Draw 1 μL sample liquid from the samp...

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Abstract

The invention relates to a thin-layer chromatographic scanner and an analysis method by use of the scanner. The thin-layer chromatographic scanner is characterized in that a reaction ionization tube and an argon pipeline are connected, a gas circuit is guaranteed to be stable through a mass flow controller; chromatobars and a tail end of a quartz tube are vertically aligned; a middle section of the reaction ionization tube is wrapped by a copper wire coil; a signal generator and a radio-frequency amplifier are coupled and connected; a radio-frequency signal of the signal generator is amplifiedand enhanced through the radio-frequency amplifier and is transmitted to the copper wire coil; a radio-frequency signal field is formed inside the copper wire coil; a reaction gas and an auxiliary gas are introduced into the reaction ionization tube; a plasma is generated under excitation of the radio-frequency signal field; a plasma wake flame sequentially scans the chromatobars which move at aconstant speed; the separated component is ionized by the plasma to produce ions; and the ion signal is collected by a collector. The thin-layer chromatographic scanner enables each chemical componentto be sequentially and rapidly detected, the radio-frequency plasma is used for thin-layer chromatographic scanning detection for the first time, the qualitativeness and quantification of each component are realized simultaneously, and thus a basis is provided for rapid understanding of chemical composition of a sample.

Description

technical field [0001] The invention relates to a novel chromatographic instrument, specifically a novel thin-layer chromatography scanner and an analysis method using the scanner, which belong to the field of petrochemical analysis and can be used in petrochemical, medical and health, environmental monitoring and fields of environmental protection. Background technique [0002] Thin-layer chromatography is a very effective means of tracking chemical reactions, and can also be used for the selection of suitable solvents in column chromatography separation. The commonly used stationary phases of thin-layer chromatography are alumina or silica gel, which are polar (normal phase) or It is non-polar (reversed phase), and the mobile phase is a polar solvent to be selected. In most industrial processes or laboratory research, such means will be used for chemical tracing or substance identification. [0003] The basic process of thin-layer chromatography is to spot the reaction mi...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 王耀辉魏晓庆
Owner 河北路科环境科技有限公司
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