Preparation method of biogenic vein containing venous valve

Abstract

The invention discloses a preparation method of a biogenic vein containing a venous valve, which is characterized in that the steps are: a. cutting the jugular vein of a cow with a diameter of 0.5 to 1.5 cm and a length of 3 to 5 cm containing a complete venous valve, and cleaning and sterilize; b. Cell lysis treatment, cell digestion and nucleic acid digestion treatment are performed on the material obtained in step a; c. The material treated in step b is sequentially subjected to covalent bonded heparin treatment → ion-bound heparin treatment → covalent bond Heparin treatment; d. Immerse the material treated in step c into the mixture of rapamycin solution and PLGA and pull it at a constant speed for 1 to 10 minutes, and then treat the vein intima with the same steps; after natural drying, put it in 20 ℃ oven drying for 12 hours. The present invention coats the venous intima and adventitia with rapamycin, so that the treated bovine jugular vein containing venous valves can significantly inhibit the hyperplasia of the venous intima and anastomosis after being implanted in the dog vein.

Description

technical field [0001] The invention relates to biomedical engineering, in particular to a method for preparing a biogenic vein containing a venous valve. Background technique [0002] Varicose veins of lower extremities is a common clinical disease, which is prone to occur in people over 45 years old. Patients rarely have obvious clinical symptoms in the early stage, but in the later stage, the superficial veins of the lower limbs may be exposed, accompanied by symptoms such as acidity, sinking, swelling, pain, and fatigue. In severe cases, superficial thrombophlebitis, stasis dermatitis, Stasis ulcer, varicose vein bleeding. The cause of varicose veins of the lower extremities is venous valve insufficiency, which leads to venous blood filling and high pressure in the lower extremities, resulting in varicose veins. Varicose veins cause local blood circulation disorders and affect the appearance of the limbs. Saphenous vein stripping, these methods are only symptomatic tre...

AI Technical Summary

Problems solved by technology

However, the experimental object of the method in the above-mentioned literature is autologous blood vessel transplantation, which does not involve immune rejection. Transplanting a vein containing a venous valve from a cow to a dog’s vein is a xenograft, and there will be greater rejection between different species, which will seriously cause veins and veins. The anastomotic stoma is narrow or even occluded, and the requirements for anti-immune rejection are higher, which cannot be met by the methods in the literature

The treatment method in the literature is to add rapamycin to the bioprotein glue and then inject it in the space around the grafted vein. Inhibiting the hyperplasia of the venous intimal, the concentration of rapamycin in the venous intimal cannot be guaranteed. In the literature, rapamycin is used after the anastomosis is completed. Different operators and different patients vary in the dosage and There are individual differences in the specific location, which is not conducive to ensuring the stability of the effect. Autologous blood vessel transplantation cannot be mass-produced on a large scale, which limits the promotion. The acquisition of autologous blood vessels will cause secondary trauma to patients that cannot be ignored.

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