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Method for reducing total nitrogen, total phosphorus and COD in low-molasses alcohol waste mash

A technology for molasses alcohol and waste mash, which is applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of loss of effective components, large land occupation, and high treatment costs, and achieve waste utilization. , the effect of reducing production costs and improving utilization

Inactive Publication Date: 2019-02-15
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The evaporation concentration method is expensive, and the waste mash after high temperature treatment has a large loss of active ingredients, so that it cannot be used reasonably and effectively
The anaerobic biogas method to treat waste mash requires long reaction residence time, large investment, and large land occupation. The main indicators such as COD, BOD, and chroma are difficult to meet the national emission standards, and the treatment cost is high, which is unaffordable for most sugar factories.

Method used

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  • Method for reducing total nitrogen, total phosphorus and COD in low-molasses alcohol waste mash

Examples

Experimental program
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Effect test

Embodiment 1

[0019] A method for reducing molasses alcohol waste mash total nitrogen, total phosphorus and COD, comprising the following steps:

[0020] (1) Culture medium preparation: Dilute the molasses alcohol waste mash to a COD value of 2000 mg / L, adjust the pH value to 7.0 with a NaOH solution with a concentration of 1mol / L, and sterilize at 121°C for 20 minutes to obtain a culture medium;

[0021] (2) Microalgae culture: inoculate the microalgae into the medium of step (1), the inoculum amount of the microalgae is 0.1g / L, and the microalgae is Monospina Monoraphidium sp. FXY-10, carried out light shake flask culture, the light intensity was 3800lux, the shaker speed was 145r / min, and the culture temperature was 23°C.

[0022] After culturing for 1 month, the culture product was centrifuged to collect the supernatant, the centrifugation speed was 3500r / min, and the centrifugation time was 5min; the COD multi-parameter photometer (Hana HI83399) was used to measure the total nitrogen, ...

Embodiment 2

[0028] A method for reducing molasses alcohol waste mash total nitrogen, total phosphorus and COD, comprising the following steps:

[0029] (1) Culture medium preparation: dilute the waste molasses alcohol mash to a COD value of 1800 mg / L, adjust the pH value to 7.0 with a NaOH solution with a concentration of 1mol / L, and sterilize at 121°C for 20 minutes to obtain a culture medium;

[0030] (2) Microalgae culture: inoculate the microalgae into the medium of step (1), the inoculum amount of the microalgae is 0.1g / L, and the microalgae is Monospina Monoraphidium sp. FXY-10, then add a concentration of 1 μmol / L melatonin mother solution with absolute ethanol as a solvent, add the concentration of melatonin in the medium to 0.1nmol / L, then carry out light shake flask culture, light The intensity is 3900lux, the rotating speed of the shaker is 150r / min, and the culture temperature is 26°C.

[0031] After culturing for 1 month, the culture product was centrifuged to collect the s...

Embodiment 3

[0034] A method for reducing molasses alcohol waste mash total nitrogen, total phosphorus and COD, comprising the following steps:

[0035] (1) Culture medium preparation: Dilute the waste molasses alcohol mash to a COD value of 2000 mg / L, adjust the pH value to 7 with a NaOH solution with a concentration of 1mol / L, and sterilize at 121°C for 20 minutes to obtain a culture medium;

[0036] (2) Microalgae culture: inoculate the microalgae into the medium of step (1), the inoculum amount of the microalgae is 0.1g / L, and the microalgae is Monospina Monoraphidium sp. FXY-10, then add a concentration of 1 μmol / L melatonin mother solution with absolute ethanol as a solvent, add the concentration of melatonin in the medium to 1nmol / L, and then carry out light shake flask culture, light intensity It is 3800lux, the rotating speed of the shaker is 145r / min, and the cultivation temperature is 23°C.

[0037] After culturing for 1 month, the culture product was centrifuged to collect the s...

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Abstract

The invention discloses a method for reducing total nitrogen, total phosphorus and COD in molasses alcohol waste mash. The method comprises the following steps: diluting the molasses alcohol waste mash till a COD value is 1800 to 2000 mg / L, adjusting the pH value to be 6.8 to 7.0 with a NaOH solution with the concentration being 1 mol / L, and performing sterilization for 20 min at 121 DEG C, so asto obtain a culture medium; inoculating microalgae into the culture medium, performing light shake-flask culture, ensuring that the light intensity is 3800 to 4200 lux, the shaker speed is 145 to 155r / min, and the culture temperature is 23 to 27 DEG C, and measuring total nitrogen, total phosphorus and COD values in the culture medium. The method has the advantages that the total nitrogen, totalphosphorus and COD values in the molasses alcohol waste mash through the microalgae, and pollution to the environment by reducing substance in the molasses alcohol waste mash is reduced; in addition,energy is saved, the treatment cost for the molasses alcohol waste mash is reduced, and the method is beneficial to environment protection.

Description

technical field [0001] The invention belongs to the technical field of using microalgae to treat organic wastewater, and in particular relates to a method for reducing total nitrogen, total phosphorus and COD of molasses alcohol waste mash. Background technique [0002] Molasses alcohol waste mash is a non-toxic, harmful, seriously polluted, and difficult to treat industrial organic wastewater. It is a by-product of alcohol production from molasses fermentation in sugar factories. It has high concentration, dark color, high acidity, and a pH value of 3.0- 5.0. As the main pollution source of sugar factories, molasses alcohol waste mash directly discharged into rivers will destroy the ecological balance. Its composition is complex, containing 8%-10% solids, of which organic substances such as sugars, amino acids, proteins account for 70%, and nitrogen , phosphorus, potassium, calcium, magnesium and other inorganic salts accounted for 30%. The treatment of molasses alcohol w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C02F3/32C12R1/89C02F103/32
CPCC12N1/12C02F3/322C02F2103/325
Inventor 余旭亚李清清赵永腾董训赞
Owner KUNMING UNIV OF SCI & TECH
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