Method for preparing cellulosic ethanol through simultaneous saccharification and fermentation
A technology for simultaneous saccharification and fermentation, cellulosic ethanol, applied in biochemical equipment and methods, microorganism-based methods, fermentation and other directions, can solve the problem of increasing fermentation cost, ethanol recovery cost, enhanced enzymatic hydrolysis product inhibition, and increased cellulase dosage. It can reduce the dosage, improve the saccharification efficiency, and increase the substrate concentration.
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Embodiment 1
[0039] The first method of synchronous saccharification and fermentation of the present invention to prepare cellulosic ethanol, this embodiment comprises the following steps:
[0040] (11). Pretreatment of lignocellulose: pretreat corn stalks with steam to obtain corn stalk biomass raw materials, then dry the biomass raw materials for 40 hours, and sieve with a 40-mesh sieve to obtain lignocellulose for later use.
[0041] (12). Preparation of mixed solvent of carboxylate zwitterionic liquid and water: prepare carboxylate zwitterionic liquid and water at a ratio of 1:5.
[0042] (13). The nanofiltration membrane divides the container into two chambers: the nanofiltration membrane is used to divide the container into a first chamber and a second chamber.
[0043] (14). Preparation of the first solvent system: Weigh 30 g of the lignocellulose prepared in step (11), and use the mixed solvent prepared in step (12) to prepare a solution with a substrate concentration of 20% and ad...
Embodiment 2
[0047] The second method of synchronous saccharification and fermentation of the present invention for preparing cellulosic ethanol, this embodiment comprises the following steps:
[0048] (21). Pretreatment of lignocellulose: Pretreat corncobs with dilute acid to obtain corncob biomass raw materials, then dry the biomass raw materials for 56 hours, sieve through 60-mesh sieves to obtain lignocellulose, and set aside .
[0049] (22). Preparation of mixed solvent of carboxylate zwitterionic liquid and water: prepare carboxylate zwitterionic liquid and water at a ratio of 1:10.
[0050] (23). The nanofiltration membrane divides the container into two chambers: the nanofiltration membrane is used to divide the container into a first chamber and a second chamber.
[0051] (24). Preparation of the first solvent system: Weigh 50 g of the lignocellulose prepared in step (21), and use the mixed solvent prepared in step (22) to make a solution with a substrate concentration of 35% and...
Embodiment 3
[0055] The third method for preparing cellulosic ethanol by synchronous saccharification and fermentation of the present invention, this embodiment includes the following steps:
[0056] (31). Pretreatment of lignocellulose: wet oxidation pretreatment of agricultural and forestry wastes to obtain biomass raw materials of agricultural and forestry wastes, then dry the biomass raw materials for 48 hours, and sieve through a 50-mesh sieve to obtain lignocellulose ,spare.
[0057] (32). Preparation of mixed solvent of carboxylate zwitterionic liquid and water: prepare carboxylate zwitterionic liquid and water at a ratio of 1:8.
[0058] (33). The nanofiltration membrane divides the container into two chambers: the nanofiltration membrane is used to divide the container into a first chamber and a second chamber.
[0059] (34). Prepare the first solvent system: weigh 40g of the lignocellulose prepared in step (31), use the mixed solvent prepared in step (32) to prepare a solution w...
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