Method for preparing cellulosic ethanol through simultaneous saccharification and fermentation

A technology for simultaneous saccharification and fermentation, cellulosic ethanol, applied in biochemical equipment and methods, microorganism-based methods, fermentation and other directions, can solve the problem of increasing fermentation cost, ethanol recovery cost, enhanced enzymatic hydrolysis product inhibition, and increased cellulase dosage. It can reduce the dosage, improve the saccharification efficiency, and increase the substrate concentration.

Active Publication Date: 2019-02-19
ZHEJIANG HUAKANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many patents have disclosed the production technology of saccharification and fermentation. For example, the patent No. CN105624208A discloses a method for producing ethanol by high-temperature enzymatic hydrolysis and fermentation of lignocellulose. Fuel ethanol is prepared by step-by-step saccharification and fermentation. The disadvantage is that the glucose produced during cellulose hydrolysis will inhibit the enzyme activity of cellulase, increase the amount of cellulase, and reduce the efficiency of enzymatic hydrolysis. Fermentation costs and ethanol recovery costs
For example, the patent of notification number CN106701863A discloses a method for synchronous enzymatic hydrolysis and fermentation of lignocellulose as raw material to prepare ethanol, and synchronous saccharification and fermentation of lignocellulose has the disadvantage that cellulase and yeast cannot be simultaneously heated at their respective suitable temperatures. When working under the same conditions, the time required for saccharification and fermentation is extended. With the progress of the enzymatic hydrolysis reaction, the concentration of reducing sugar gradually increases, and the inhibitory effect of enzymatic hydrolysis products is enhanced, thereby affecting the yield of reducing sugar, and the fermentation efficiency decreases at the same time.
At the same time, the by-products of lignocellulose pretreatment, such as organic acids, furans and phenols, have not been effectively removed, making these compounds significantly inhibit the growth and fermentation of yeast, thus making ethanol Yield reduction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The first method of synchronous saccharification and fermentation of the present invention to prepare cellulosic ethanol, this embodiment comprises the following steps:

[0040] (11). Pretreatment of lignocellulose: pretreat corn stalks with steam to obtain corn stalk biomass raw materials, then dry the biomass raw materials for 40 hours, and sieve with a 40-mesh sieve to obtain lignocellulose for later use.

[0041] (12). Preparation of mixed solvent of carboxylate zwitterionic liquid and water: prepare carboxylate zwitterionic liquid and water at a ratio of 1:5.

[0042] (13). The nanofiltration membrane divides the container into two chambers: the nanofiltration membrane is used to divide the container into a first chamber and a second chamber.

[0043] (14). Preparation of the first solvent system: Weigh 30 g of the lignocellulose prepared in step (11), and use the mixed solvent prepared in step (12) to prepare a solution with a substrate concentration of 20% and ad...

Embodiment 2

[0047] The second method of synchronous saccharification and fermentation of the present invention for preparing cellulosic ethanol, this embodiment comprises the following steps:

[0048] (21). Pretreatment of lignocellulose: Pretreat corncobs with dilute acid to obtain corncob biomass raw materials, then dry the biomass raw materials for 56 hours, sieve through 60-mesh sieves to obtain lignocellulose, and set aside .

[0049] (22). Preparation of mixed solvent of carboxylate zwitterionic liquid and water: prepare carboxylate zwitterionic liquid and water at a ratio of 1:10.

[0050] (23). The nanofiltration membrane divides the container into two chambers: the nanofiltration membrane is used to divide the container into a first chamber and a second chamber.

[0051] (24). Preparation of the first solvent system: Weigh 50 g of the lignocellulose prepared in step (21), and use the mixed solvent prepared in step (22) to make a solution with a substrate concentration of 35% and...

Embodiment 3

[0055] The third method for preparing cellulosic ethanol by synchronous saccharification and fermentation of the present invention, this embodiment includes the following steps:

[0056] (31). Pretreatment of lignocellulose: wet oxidation pretreatment of agricultural and forestry wastes to obtain biomass raw materials of agricultural and forestry wastes, then dry the biomass raw materials for 48 hours, and sieve through a 50-mesh sieve to obtain lignocellulose ,spare.

[0057] (32). Preparation of mixed solvent of carboxylate zwitterionic liquid and water: prepare carboxylate zwitterionic liquid and water at a ratio of 1:8.

[0058] (33). The nanofiltration membrane divides the container into two chambers: the nanofiltration membrane is used to divide the container into a first chamber and a second chamber.

[0059] (34). Prepare the first solvent system: weigh 40g of the lignocellulose prepared in step (31), use the mixed solvent prepared in step (32) to prepare a solution w...

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Abstract

The invention relates to a method for preparing cellulosic ethanol through simultaneous saccharification and fermentation. The method comprises the following steps of partitioning the interior of a container into a first accommodating cavity and a second accommodating cavity in advance through a nanofiltration membrane or an ultrafiltration membrane, wherein the first accommodating cavity is filled with a first solvent system of enzymolysis fermentation solvents, and the second accommodating cavity is filled with a second solvent system of fermentation solvents; adding pretreated lignocellulose into the first solvent system of the first accommodating cavity, and after the first solvent system and the second solvent system are stirred for 12-60 h separately, simultaneously obtaining cellulosic ethanol after fermentation and saccharification in the two accommodating cavities respectively. According to the method, lignocellulose serves as a raw material, carboxylate zwitterionic liquid and water are regarded as a mixed solvent, and cellulose is added; meanwhile, the mixed solvent is connected with a solvent system formed by co-fermentation yeast of a non-ionic surfactant and C5 and C6through the nanofiltration membrane to achieve the purposes of high saccharification and high fermentation.

Description

technical field [0001] The invention belongs to the technical field of biomass cellulose ethanol preparation, in particular to a method for synchronous saccharification and fermentation to prepare cellulose ethanol. Background technique [0002] Lignocellulosic raw material is an extremely abundant renewable resource in nature, and its main components are cellulose, hemicellulose and lignin. The production of biofuels or bio-based chemicals from sugar polymers in lignocellulose is an effective way for the comprehensive utilization of lignocellulose. Using lignocellulosic raw materials to convert fuel ethanol to partially replace depleted fossil energy can alleviate energy crisis, environmental pollution and other problems. If lignocellulose can be well utilized, it will greatly reduce the cost of chemical raw materials and automobile fuel. However, until now, the utilization of lignocellulosic raw materials has been insufficient. This is because lignocellulosic raw mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/10C12P19/14C12R1/84C12R1/72C12R1/865
CPCC12P7/10C12P19/14C12P39/00C12P2201/00Y02E50/10Y02P20/54
Inventor 张金建李勉杨世辉黄涛丁士友李哲江雪松郑晓阳
Owner ZHEJIANG HUAKANG PHARMA
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