Method for extracting shikimic acid from anise
A technology of shikimic acid and star anise, applied in the field of extraction and purification of plant active ingredients, can solve problems such as increased difficulty in solvent regeneration and utilization, poor product quality and appearance, and difficulty in guaranteeing product purity, and achieves uniform size, pure color, and required time. the effect of shortening
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Embodiment 1
[0027] Take 100g star anise raw material and add 4 times the weight of water with a high-pressure homogenizer to crush 3 times, each time for 10 minutes. When the temperature of the reaction kettle is adjusted to 45°C, add pectinase. The dosage of pectinase is 1 / 1000 of the raw material weight. Enzymolyze at 45°C for 1 hour and collect the enzymatic hydrolyzate. The organic membrane of 8000 molecular weight, membrane inlet pressure 0.45MPa, the filtrate that collects is passed through the organic membrane that can cut off 800 molecular weight, membrane inlet pressure 1.8MPa, obtains the decolorization membrane liquid, membrane fluid adds 0.1g yeast, described yeast ( Purchased from Angel Yeast (Chongzuo) Co., Ltd.) and fermented at 30°C for 36 hours under aerobic conditions, the obtained fermentation broth was concentrated under reduced pressure until the solid content in the obtained concentrated solution was 55% brix, and the volume fraction was 4% sulfuric acid Adjust the p...
Embodiment 2
[0029] Get 100g star anise raw material and add 5 times the weight of water with a high-pressure homogenizer to crush twice, each time for 20 minutes. When the temperature of the reaction kettle is adjusted to 46°C, two enzymes, cellulase and papain, are added, wherein the total amount of cellulase and papain is 3 / 1000 of the weight of the raw material, and the enzyme is hydrolyzed at 48°C for 2 hours, and collected Enzymolysis solution, the obtained enzymolysis solution first passes through an organic membrane that can cut off 8000 molecular weight, and the membrane inlet pressure is 0.48MPa, and the collected filtrate is passed through an organic membrane that can cut off 800 molecular weight, and the membrane inlet pressure is 1.9MPa, to obtain a decolorized membrane solution. 1 g of yeast (purchased from Angel Yeast (Chongzuo) Co., Ltd.) was added to the membrane-passing liquid and fermented at 32° C. for 40 hours under aerobic conditions. The obtained fermentation liquid w...
Embodiment 3
[0031] Take 100g star anise raw material and add 3 times the weight of water to crush once with a high-pressure homogenizer, 30 minutes each time. When the temperature of the reaction kettle is adjusted to 48°C, three enzymes, cellulase, papain, and amylase, are added. The total amount of the above three enzymes is 5 / 1000 of the weight of the raw material, and the enzyme is hydrolyzed at 60°C for 3 hours. , collect the enzymolysis solution, the obtained enzymolysis solution first passes through an organic membrane with a molecular weight cut-off of 8000, and the membrane inlet pressure is 0.5MPa, and the collected filtrate passes through an organic membrane with a molecular weight cut-off of 800, and the membrane inlet pressure is 2.0MPa, to obtain a decolorized membrane 1.5 g of yeast (purchased from Angel Yeast (Chongzuo) Co., Ltd.) was added to the membrane-passing liquid and fermented at 35°C for 48 hours under aerobic conditions. The obtained fermentation liquid was concen...
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