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Extraction method of auricularia auricula polysaccharides and preparing method of preparations of auricularia auricula polysaccharides

A fungus polysaccharide and extraction method technology, applied in the field of biomedicine, can solve the problems of loss of active components, difficult separation of material and liquid, unfavorable dissolution of active components, etc., and achieve the effect of improving yield

Inactive Publication Date: 2019-03-19
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the scientific research institutes of colleges and universities and many enterprises have carried out relevant research on the extraction of black fungus by using modern science and technology, they are all in the laboratory scale and stage, mainly because the black fungus extraction system is extremely viscous under normal circumstances, and the separation of material and liquid is difficult. , the extraction efficiency is extremely low, and it is difficult to achieve large-scale production; the difficulty in separating the material and liquid leads to a long extraction cycle, the loss or deterioration of active ingredients, and the existing black fungus extract related products are not only of poor quality, but also seriously adulterated
[0004] At present, the research methods for extracting black fungus polysaccharides mainly include: hot water extraction method, alkali extraction method, enzymatic method, ultrasonic-assisted extraction method, microwave-assisted extraction method, etc. Facilitates the dissolution of active ingredients
Using hot water to extract polysaccharides is a simple and easy method with low cost but low yield and time-consuming
Alkaline extraction of polysaccharides can shorten the time and increase the yield, but if the pH is too high, the biological activity of polysaccharides is easily lost
Enzymatic extraction uses cellulase, pectinase and neutral protease combined with hot water extraction. Although the yield of black fungus polysaccharides is high, the molecular structure of black fungus polysaccharides is destroyed, and the molecular weight of the extracted polysaccharides is greatly changed. Small, thus affecting the efficacy of black fungus polysaccharides

Method used

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  • Extraction method of auricularia auricula polysaccharides and preparing method of preparations of auricularia auricula polysaccharides
  • Extraction method of auricularia auricula polysaccharides and preparing method of preparations of auricularia auricula polysaccharides
  • Extraction method of auricularia auricula polysaccharides and preparing method of preparations of auricularia auricula polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Bake 500g of dry black fungus raw material at a temperature of 75°C for 180min, then ultrafinely pulverize it to a particle size of 100 mesh, add water 40 times the weight ratio and soak for 30min, extract at a temperature of 100°C, extract for 1h, and centrifuge the extract at 5000rpm , vacuum-dried to obtain 52.8g of fungus polysaccharide, which was crushed into fine powder, added an appropriate amount of auxiliary materials such as starch and dextrin, mixed evenly, made into granules, and packed into hard capsules to obtain 1000 capsules.

Embodiment 2

[0028] Bake 800g of dried black fungus raw material at a temperature of 95°C for 30 minutes, then ultrafinely pulverize it to a particle size of 200 mesh, add water 30 times the weight ratio and soak for 90 minutes, extract at a temperature of 95°C, extract for 3 hours, and centrifuge the extract at 3000rpm , vacuum-dried to obtain 72.2g of fungus polysaccharide, crushed into fine powder, added appropriate amount of starch, dextrin and other auxiliary materials, mixed, wet granulated, granulated after drying, packaged to obtain 1000g granules.

Embodiment 3

[0030] Bake 650g of dried black fungus raw material at a temperature of 85°C for 60 minutes, then ultrafinely pulverize it to a particle size of 160 mesh, add water 35 times the weight ratio and soak for 60 minutes, extract at a temperature of 98°C, extract for 2 hours, and centrifuge the extract at 4000rpm , vacuum-dried to obtain 78.1g of fungus polysaccharide, crushed into fine powder, added appropriate amount of starch, dextrin and other auxiliary materials, mixed evenly, made into granules, and pressed into tablets to obtain 1000 preparations.

[0031] Example 1

[0032] 650g of dried black fungus raw materials were ultrafinely pulverized to a particle size of 160 mesh, soaked in 35 times of water by weight for 60 minutes, extracted at 98°C for 2 hours, centrifuged at 4000rpm, and dried in vacuum to obtain 35.9g of fungus polysaccharides. Crush it into fine powder, add appropriate amount of auxiliary materials such as starch and dextrin, mix well, make granules, and press...

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Abstract

The invention provides an extraction method of auricularia auricula polysaccharides and a preparing method of preparations of auricularia auricula polysaccharides and belongs to the field of biological medicine. The extraction method includes the steps of baking raw material of auricularia auricula, superfine grinding, extracting by adding water, and centrifuging to obtain auricularia auricula polysaccharides. Compared with the prior art, the yield and the .02- clearance rate of the auricularia auricula polysaccharides prepared according to the technical scheme are increased greatly.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for extracting polysaccharides from fungus and a preparation method thereof. Background technique [0002] With the advancement of science and technology and the improvement of living standards, people's pursuit of high-quality life, health and comfort is becoming more and more intense. Fungal polysaccharides are currently the most promising new resource for health food and medicine. Scientific experiments have shown that fungal polysaccharides have anti-tumor activity, strong inhibitory effect on cancer cells, and play an important role in immune regulation, lowering blood pressure, lowering blood fat, and antithrombotic. At present, the research on the relationship between the structure, properties and pharmacodynamics of polysaccharides has become one of the most active research fields in life sciences. The main active ingredient in black fungus - black fungus polysacchar...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P39/06
CPCA61K31/715A61P39/06C08B37/0003C08B37/006
Inventor 孔祥辉张介驰马银鹏于德水韩冰陈鹤杨国力王天亮张丕奇戴肖东
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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