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A kind of fermented collagen peptide and preparation method thereof

A collagen peptide and fermentation broth technology, applied in food science and other directions, can solve the problems that consumers are unacceptable, the collagen peptide is bitter and fishy, ​​and has poor palatability, and achieves good palatability, removal of fishy smell, The effect of improving the extraction rate

Active Publication Date: 2022-03-11
厦门元之道生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At this stage, collagen peptides produced by traditional techniques have a heavy bitter and fishy smell, poor palatability, and are difficult for consumers to accept

Method used

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  • A kind of fermented collagen peptide and preparation method thereof
  • A kind of fermented collagen peptide and preparation method thereof
  • A kind of fermented collagen peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh 2g of black tea powder and 2g of perilla powder, add 1000mL of water, boil and extract for 25min, filter to obtain plant extract;

[0024] Soak tilapia fish skin and plant extract at a ratio of 1:1 for 1 hour, drain and set aside;

[0025] Add distilled water according to the weight ratio of material to liquid 1:8, and beat with a beater;

[0026] The slurry after beating was placed in a colloid mill for homogenization to obtain uniform tilapia fish skin slurry, which was pumped to a high-voltage pulsed electric field treatment chamber through a peristaltic pump at a flow rate of 8mL / min, with an electric field intensity of 15KV / cm and a pulse frequency of 20Hz The pulse time is 1s for processing to obtain a high-voltage pulsed electric field (Pulsed Electric Fields, check PEF) treatment solution;

[0027] The obtained PEF treatment solution was treated at 121°C for 30 minutes to kill pathogenic bacteria, and then cooled to 35°C to obtain a liquid culture medium;...

Embodiment 2

[0033] Weigh 2g of black tea powder and 2g of perilla powder, add 1000mL of water, boil and extract for 25min, filter to obtain plant extract;

[0034] Soak tilapia fish skin and plant extract at a ratio of 1:1 for 1 hour, drain and set aside;

[0035] Add distilled water according to the weight ratio of material to liquid 1:8, and beat with a beater;

[0036] The beating slurry is placed in a colloid mill for homogenization to obtain a uniform tilapia skin slurry. Pumped to the high-voltage pulsed electric field treatment chamber through a peristaltic pump at a flow rate of 8mL / min, treated with an electric field intensity of 20KV / cm, a pulse frequency of 20Hz, and a pulse time of 1S to obtain a PEF treatment solution;

[0037] The obtained PEF treatment solution was treated at 121°C for 30 minutes to kill pathogenic bacteria, and then cooled to 35°C to obtain a liquid culture medium;

[0038] Inoculate the activated slant strain into the seed culture medium, ferment and cu...

Embodiment 3

[0043] Weigh 2g of black tea powder and 2g of perilla powder, add 1000mL of water, boil and extract for 25min, filter to obtain plant extract;

[0044] Soak tilapia fish skin and plant extract at a ratio of 1:1 for 1 hour, drain and set aside;

[0045] Add distilled water according to the weight ratio of material to liquid at 1:10, and beat with a beater;

[0046] The slurry after beating was placed in a colloid mill for homogenization to obtain a uniform tilapia fish skin slurry, which was pumped to a high-voltage pulsed electric field treatment chamber through a peristaltic pump at a flow rate of 8mL / min, with an electric field intensity of 25KV / cm and a pulse frequency of 30Hz The pulse time is 1s for processing to obtain the PEF treatment solution;

[0047] The obtained PEF treatment solution was treated at 121°C for 30 minutes to kill pathogenic bacteria, and then cooled to 35°C to obtain a liquid culture medium;

[0048] Inoculate the activated slant strain into the se...

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Abstract

The invention relates to the technical field of biological applications, in particular to a fermented collagen peptide and a preparation method thereof. The preparation method comprises the following steps: using tilapia skin as a raw material, soaking and draining the plant extract, and then performing beating and colloid mill treatment; the material treated by beating and colloid mill is then treated with high-voltage pulse electric field; the obtained high-voltage The pulse electric field treatment solution is sterilized; after the sterilization, the pressure pulse electric field treatment solution is fermented with Bacillus subtilis to obtain a fermentation liquid; the fermentation liquid is ultrafiltered, concentrated, and spray-dried to obtain a fermented collagen peptide. The preparation method of the collagen peptide provided by the present invention can produce a fermented collagen peptide product with no fishy smell, a fermented flavor, and good palatability by soaking in plant extracts, extracting in a high-voltage pulse electric field, and fermenting with Bacillus subtilis. It has a certain skin-beautifying and moisturizing effect, and the prepared collagen peptide can be used as a food raw material in the form of stock solution and collagen powder.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to a fermented collagen peptide and a preparation method thereof. Background technique [0002] Collagen is a high-molecular functional protein widely distributed in human connective tissue, skin, bones, etc., accounting for 25-30% of the total protein in the human body. Collagen peptide is a collagen precursor substance obtained from collagen or gelatin by protease degradation. Its molecular weight is much smaller than that of collagen, and it is easier to be digested and absorbed by the human body. Because of its good absorption and solubility, it has a wide range of applications in biomedicine, beauty, and functional products. [0003] At this stage, collagen peptides produced by traditional techniques have a strong bitter and fishy smell, poor palatability, and are difficult for consumers to accept. Contents of the invention [0004] In order to solve the pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/00
CPCA23L33/00
Inventor 黄君阳
Owner 厦门元之道生物科技有限公司
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