Method for preparing microalgae grease through mixed cultivation

A technology of mixed culture and microalgae oil, which is applied in the field of biotechnology and bioenergy, can solve the problems of unfavorable algae absorption and utilization, no oil content involved, and lower carbon fixation efficiency, so as to alleviate the greenhouse effect and exhaust gas pollution, and suppress miscellaneous The growth of bacteria and the effect of improving carbon sequestration efficiency

Active Publication Date: 2019-04-05
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
[0005] But in practical application, when the environment CO 2 When the volume fraction is greater than 5v%, the growth of most microalgae will be inhibited, and the carbon fixation efficiency will be low
At the same time, general microalgae are suitable for growth under neutral conditions, and are no

Method used

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  • Method for preparing microalgae grease through mixed cultivation
  • Method for preparing microalgae grease through mixed cultivation
  • Method for preparing microalgae grease through mixed cultivation

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[0022] The preparation process of the microalgae seed liquid of the present invention is as follows: the microalgae culture adopts BG11 medium, and the medium formula is shown in Table 1 and Table 2.

[0023] Table 1 BG11 medium

[0024]

[0025] *Table 2 Composition of A5+Co solution in Table 1

[0026]

[0027] First, prepare the BG11 liquid medium according to Table 1 and Table 2, adjust the pH of the medium for culturing Pseudochlorella kelsii FSH-Y3 and Scenedesmus obliquus FSH-Y2 to 10, and adjust the culture medium of Chlorella SF-B1 The pH of the culture medium was adjusted to 8.0, and then the Chlorella kessleri FSH-Y3, Scenedesmus obliquus FSH-Y2, and Chlorella SF-B1 were respectively inoculated into the medium. Cultivate in a constant temperature light shaker with a culture temperature of 25°C, a photoperiod of 24h, a light-dark time ratio of 14:10, a light intensity of 5000 Lux, and 120 rpm shaking culture to the logarithmic growth phase to obtain Pseudochlorella kelvin ...

Example Embodiment

[0028] Example 1

[0029] The microalgae seed liquid prepared above was respectively inoculated into BG11 medium to produce microalgae oil. In a 20L airlift photobioreactor, the seed solution of Chlorella kelsii FSH-Y3 and Chlorella SF-B1 are inoculated at a volume ratio of 1:1, and 20% of the total inoculation amount is inoculated to In the BG11 medium, the pH value is controlled between 8-11, and a mixed gas of nitrogen and carbon dioxide is introduced. The carbon dioxide content is 5v%-4v%, the light intensity is 5000 Lux, the culture temperature is 20°C, and the light cycle is 24h. The ratio of light to dark time is 14:10. After 7 days of cultivation, it is in a stable period. The cultivation is finished, the algae liquid is collected by centrifugation, and the dry weight of the algae powder is measured after vacuum freeze-drying to a constant weight at -60℃. The biomass yield is calculated and measured by the n-hexane:ethyl acetate method The total fat content is shown in ...

Example Embodiment

[0033] Example 2

[0034] The microalgae seed liquid prepared above was respectively inoculated into BG11 medium to produce microalgae oil. In a 20L airlift photobioreactor, the seed solution of Scenedesmus obliquus FSH-Y2 and Chlorella SF-B1 are inoculated at a volume ratio of 1:1, and 20% of the total inoculation amount is inoculated to BG11 for cultivation. In the base, the pH value is controlled between 8-11, and a mixed gas of nitrogen and carbon dioxide is introduced. The carbon dioxide content is 5v%-4v%, the light intensity is 5000 Lux, the culture temperature is 20℃, the light cycle is 24h, and the light is dark. The time ratio is 14:10. After 7 days of cultivation, it is in a stable period. The cultivation is finished, the algae liquid is collected by centrifugation, and the dry weight of the algae powder is measured after vacuum freeze-drying to a constant weight at -60℃. The biomass yield is calculated and measured by the n-hexane:ethyl acetate method. The total fat...

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Abstract

The invention relates to a method for preparing microalgae grease through mixed cultivation. The method comprises the steps that a microalgae culture medium and a mixed microalgae seed solution are added in a photobioreactor, the pH of a cultivation system is maintained to be 8-12, preferably, 9-11, and CO2 with the volume content being 5v%-45v%, preferably, 10v%-30v%, is introduced; mixed microalgae comprises chlorella sp. SF-B1, and meanwhile comprises parachlorella kessleri FSH-Y3 or/and scenedesmus obliqnus FSH-Y2. According to the method, the tolerance and solubleness of the microalgae culture cultivation system to high-concentration CO2 are improved, carbon fixation efficiency is improved, the harvest yield of the microalgae grease is obviously improved, and production of biodiesel is facilitated.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for preparing microalgae oil by mixed culture. Background technique [0002] As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a potential resource for the production of pharmaceuticals, fine chemicals and new fuels. The fatty acids obtained from microalgae can be converted into fatty acid methyl ester, that is, biodiesel. Therefore, b...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/64C12P7/6463C12P7/649Y02E50/10
Inventor 师文静王鹏翔李晓姝孙启梅张霖樊亚超
Owner CHINA PETROLEUM & CHEM CORP
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