Method for preparing microalgae grease through mixed cultivation

Abstract

The invention relates to a method for preparing microalgae grease through mixed cultivation. The method comprises the steps that a microalgae culture medium and a mixed microalgae seed solution are added in a photobioreactor, the pH of a cultivation system is maintained to be 8-12, preferably, 9-11, and CO2 with the volume content being 5v%-45v%, preferably, 10v%-30v%, is introduced; mixed microalgae comprises chlorella sp. SF-B1, and meanwhile comprises parachlorella kessleri FSH-Y3 or / and scenedesmus obliqnus FSH-Y2. According to the method, the tolerance and solubleness of the microalgae culture cultivation system to high-concentration CO2 are improved, carbon fixation efficiency is improved, the harvest yield of the microalgae grease is obviously improved, and production of biodiesel is facilitated.

Description

technical field

[0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for preparing microalgae oil by mixed culture. Background technique

[0002] As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a potential resource for the production of pharmaceuticals, fine chemicals and new fuels. The fatty acids obtained from microalgae can be converted into fatty acid methyl ester, that is, biodiesel. Therefore, b...

Examples

preparation example Construction

[0022] The preparation process of the microalgae seed solution of the present invention is as follows: the microalgae culture adopts BG11 medium, and the formulation of the medium is shown in Table 1 and Table 2.

[0023] Table 1 BG11 medium

[0024]

[0025] *Table 2 Composition of A5+Co solution in Table 1

[0026]

[0027] First, prepare BG11 liquid medium according to Table 1 and Table 2, adjust the pH of the medium for cultivating Pseudochlorella kesserii FSH-Y3 and Scenedesmus obliquus FSH-Y2 to 10, and adjust the pH of the medium for cultivating Chlorella keserii SF-B1 The pH of the medium was adjusted to 8.0, and then Pseudochlorella kescherei FSH-Y3, Scenedesmus obliquus FSH-Y2, and Chlorella SF-B1 were inoculated in the above-mentioned medium respectively. Cultivate in a constant temperature light shaker, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, 120rpm shaking culture until the...

Embodiment 1

[0029]The prepared microalgae seed liquids were respectively inoculated in BG11 medium to produce microalgae oil. In a 20L air-lift photobioreactor, the seed liquid of Pseudochlorella kesperi FSH-Y3 and the seed liquid of Chlorella SF-B1 were inoculated according to the volume ratio of 1:1, and the total inoculation amount of 20% was inoculated to In the BG11 medium, the pH value is controlled between 8 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the culture temperature is 20°C, and the light cycle is 24h. The light to dark time ratio is 14:10. After cultivating for 7 days, it is in a stable period. After cultivating, the algae liquid is collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder is measured to calculate the biomass production, which is measured by the n-hexane:ethyl acetate method The total lipid c...

Embodiment 2

[0034] The prepared microalgae seed liquids were respectively inoculated in BG11 medium to produce microalgae oil. In a 20L airlift photobioreactor, Scenedesmus obliquus FSH-Y2 seed liquid and Chlorella SF-B1 seed liquid were inoculated at a ratio of 1:1 by volume, and were inoculated to BG11 at a total inoculum of 20%. In the medium, the pH value is controlled between 8 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the cultivation temperature is 20°C, the light cycle is 24h, and the light is dark. The time ratio is 14:10. After cultivating for 7 days, it is in a stable period. After cultivating, the algae liquid is collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder is measured to calculate the biomass production, which is measured by the n-hexane:ethyl acetate method The total lipid content, the results are ...

technical field

[0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for preparing microalgae oil by mixed culture. Background technique

[0002] As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a potential resource for the production of pharmaceuticals, fine chemicals and new fuels. The fatty acids obtained from microalgae can be converted into fatty acid methyl ester, that is, biodiesel. Therefore, b...

Examples

preparation example Construction

[0022] The preparation process of the microalgae seed solution of the present invention is as follows: the microalgae culture adopts BG11 medium, and the formulation of the medium is shown in Table 1 and Table 2.

[0023] Table 1 BG11 medium

[0024]

[0025] *Table 2 Composition of A5+Co solution in Table 1

[0026]

[0027] First, prepare BG11 liquid medium according to Table 1 and Table 2, adjust the pH of the medium for cultivating Pseudochlorella kesserii FSH-Y3 and Scenedesmus obliquus FSH-Y2 to 10, and adjust the pH of the medium for cultivating Chlorella keserii SF-B1 The pH of the medium was adjusted to 8.0, and then Pseudochlorella kescherei FSH-Y3, Scenedesmus obliquus FSH-Y2, and Chlorella SF-B1 were inoculated in the above-mentioned medium respectively. Cultivate in a constant temperature light shaker, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, 120rpm shaking culture until the...

Embodiment 1

[0029]The prepared microalgae seed liquids were respectively inoculated in BG11 medium to produce microalgae oil. In a 20L air-lift photobioreactor, the seed liquid of Pseudochlorella kesperi FSH-Y3 and the seed liquid of Chlorella SF-B1 were inoculated according to the volume ratio of 1:1, and the total inoculation amount of 20% was inoculated to In the BG11 medium, the pH value is controlled between 8 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the culture temperature is 20°C, and the light cycle is 24h. The light to dark time ratio is 14:10. After cultivating for 7 days, it is in a stable period. After cultivating, the algae liquid is collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder is measured to calculate the biomass production, which is measured by the n-hexane:ethyl acetate method The total lipid c...

Embodiment 2

[0034] The prepared microalgae seed liquids were respectively inoculated in BG11 medium to produce microalgae oil. In a 20L airlift photobioreactor, Scenedesmus obliquus FSH-Y2 seed liquid and Chlorella SF-B1 seed liquid were inoculated at a ratio of 1:1 by volume, and were inoculated to BG11 at a total inoculum of 20%. In the medium, the pH value is controlled between 8 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the cultivation temperature is 20°C, the light cycle is 24h, and the light is dark. The time ratio is 14:10. After cultivating for 7 days, it is in a stable period. After cultivating, the algae liquid is collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder is measured to calculate the biomass production, which is measured by the n-hexane:ethyl acetate method The total lipid content, the results are ...

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