Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for increasing fermentation yield of L-leucine

A leucine and production technology, which is applied in the field of increasing the fermentation production of L-leucine, can solve the problems that the acid production of fermentation needs to be improved, the mixed fermentation process is cumbersome, and the fermentation parameters are difficult to control accurately.

Inactive Publication Date: 2019-04-12
XINJIANG FUFENG BIOTECH +2
View PDF7 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biotin on Brevibacterium flavum L- The effect of Leu has not been reported
[0005] applicant to L- Leu has carried out comparatively sufficient research, and the previous patented technology "a method for continuously extracting leucine using sequential simulated moving bed chromatography" discloses a process for fermenting leucine, including corynebacterium glutamicum seed liquid and The Bacillus subtilis seed liquid is mixed according to a certain volume ratio to obtain a mixed seed liquid, and then transferred to a fermentation medium for cultivation at a temperature of 30°C, a pH value of 7.2, and a cultivation time of 60 hours to obtain a leucine fermentation liquid; this method is relatively simple Compared with the fermentation method, the efficiency of fermentation to produce leucine is improved, but there are defects such as cumbersome mixed fermentation process and difficulty in precise control of fermentation parameters; Chinese invention patent "CN104404495A" discloses a method for producing L-leucine by fermentation. In this method, by optimizing the fermentation medium and fermentation parameters, the leucine yield in the fermentation broth can reach 38g / L. However, this method is a small-scale fermentation tank pilot test process, and there are many uncertainties in the effect of expanding fermentation culture, and Fermentation acid production still needs to be improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for increasing fermentation yield of L-leucine
  • Method for increasing fermentation yield of L-leucine
  • Method for increasing fermentation yield of L-leucine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Test strain: Brevibacterium flavum ( Brevibacterium flavum ) TK0303 (Met - +Ile L +2-TA r +α-AB r +β-HL r +Rif r +SG r ), preserved by the Laboratory of Metabolic Engineering, Tianjin University of Science and Technology.

[0031] main instrument

[0032] Fermentation tank (5 L, 30 L automatic fermentation tank, Shanghai Baoxing Biological Equipment Engineering Company); biological sensor (SBA-40C, Institute of Biology, Shandong Academy of Sciences); pH electrode (METTLER TOLEDO); dissolved oxygen electrode (METTLER TOLEDO); high performance liquid chromatography (LC20AT, Shimadzu); TU 1810 UV-Vis spectrophotometer (Beijing General Instrument); electronic balance (FA2204B, Shanghai Precision Scientific Instrument Co., Ltd.).

[0033] training method

[0034] 1. Seed cultivation

[0035] Draw an appropriate amount of sterile normal saline into 5 activated slopes, put the bacterial suspension into a 5 L seed tank, the initial ventilation rate is 1L / min; the sti...

Embodiment 2

[0041] Put the seed liquid into a 30 L fermenter with 24 L fermenter culture medium according to 10% inoculation amount; the ventilation rate is 2 L / min; the stirring speed is 500 r / min; the pH is controlled at 7.0 by automatic flow of ammonia water; the culture temperature 33°C; use foam to defoam; when the fermentation reaches 20 hours, add the nutrient solution flow into the tank to maintain the sugar concentration in the tank at 1-2g / L; couple the fermenter with a ceramic membrane, and ferment to 36 hours , the fermented liquid in the fermenter is separated by the ceramic membrane, the dialysis filtrate is discharged, the concentrated bacteria are returned to the fermenter, and the fermenter medium is added to the fermenter at the same time, so that the fermented liquid before separation by the ceramic membrane The volume is the same, and the fermentation is continued for 20 hours to complete the fermentation.

[0042] Components of the fermenter culture medium: glucose 50...

Embodiment 3

[0044] Analytical method

[0045] 1. Determination of bacterial concentration

[0046] An appropriate amount of fermentation broth was taken every 4 h, diluted with sterilized physiological saline, and the absorbance at 600 nm wavelength was measured by a spectrophotometer.

[0047] 2. Determination of dissolved oxygen and pH

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of the production of amino acids, and discloses a method for increasing fermentation yield of L-leucine. The method comprises the following steps of inoculating culture mediums containing biotin in a fermenter with brevibacterium flavum, and performing fermentation. According to the method disclosed by the invention, the metabolic pathway of the L-leucine is analyzed, so that the fermentation culture medium is optimized, fermentation steps are optimized, and the acid yield through fermentation can be increased.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a method for increasing the fermentation yield of L-leucine. Background technique [0002] L-leucine ((2R)-2-amino-3,3-dimethylbutanoic acid) chemical name is L-2-ammonia-4-methylpentanoic acid, white crystal or crystalline powder; odorless, slightly bitter taste . Soluble in formic acid, slightly soluble in water, very slightly soluble in ethanol or ether. The density is 1.038g / cm 3 , melting point greater than 300 ° C. [0003] Of the 20 common amino acids, L -Leucine( L -Leu) with L - Isoleucine, L - Valine is called a branched chain amino acid [1] . Branched-chain amino acids are essential amino acids for the human body. They are the materials for the synthesis of proteins. They can be used as energy sources for living organisms and precursors of biological components. Biosynthetic pathways of three branched-chain amino acids, partly sharin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P13/06C12N1/20C12R1/13
CPCC12N1/20C12P13/06
Inventor 包鑫徐庆阳李德衡王健张宗华杨瑞丽张玉福
Owner XINJIANG FUFENG BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com