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Method for preparing bioethanol from bloom-forming cyanobacteria

A technology of blooming cyanobacteria and bioethanol, which is applied in the fields of bioenergy technology and environmental protection to achieve the effects of increasing sugar content, increasing yield, and solving ecological and environmental problems

Active Publication Date: 2019-04-16
EAST CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the relevant technology in this field is still blank, because the lipid and sugar content of wild cyanobacteria is lower than that of artificially cultivated cyanobacteria. For example, the lipid content of cyanobacteria salvaged from Taihu Lake is less than 1%, and the sugar content is low. It is less than 10%, so it is not feasible to directly use it as a raw material for the production of biofuels

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  • Method for preparing bioethanol from bloom-forming cyanobacteria

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Effect test

Embodiment 1

[0028] Salvage and collect the cyanobacteria in the Yangwan area of ​​Taihu Lake when the water blooms broke out. The absolute dominant species is Microcystis, and the impurities visible to the naked eye are removed. Inoculate the collected bloom cyanobacteria into the photobioreactor, add Taihu Lake water to make the chlorophyll concentration 2-4mg / L, add NaNO 3 and K 2 HPO 3 , placed at 6000 lx, and cultured at 28°C. Regularly measure the dry weight and soluble total sugar production to calculate the sugar content; measure the chemical oxygen demand (COD) and biochemical oxygen demand (BOD) of the algae cell contents, and calculate the BOD / COD to characterize its biodegradability.

[0029] Under the condition of nitrogen-phosphorus ratio of 10:1, adding 0.01-0.05‰ photosynthesis accelerator and 1-3‰ metabolism disruptor, the dry weight increased from 0.527 g / L to 1.758 g / L, and the total soluble sugar increased from 33.7 mg / L to 995.6 mg / L. Compared with uncultured cyano...

Embodiment 2

[0031] Dissolve 1.2 g of the recultivated algae powder directly in 40 ml of 2mol / L hydrochloric acid solution to prepare a 30 g / L algae solution. High temperature hydrolysis at 121°C for 30 min, and the hydrolyzate was filtered through a 0.45 μm filter membrane. The changes of soluble total sugar and reducing sugar before and after hydrolysis were measured. The soluble total sugar and reducing sugar of the recultivated algal flour before hydrolysis were 17.0 g / L and 1.9 g / L, accounting for 56.6% and 6.4% of the dry weight, respectively; after hydrolysis, they were 17.9 g / L and 17.3 g / L , accounting for 59.8% and 57.5% of the dry weight, respectively. After hydrolysis, the proportion of reducing sugar in total sugar increased from 10.9% to 96.2%.

[0032] At the same time, the uncultivated algal powder was treated under the same conditions, and it was found that the total soluble sugar and reducing sugar before hydrolysis were 1.9 g / L and 1.2 g / L, accounting for 6.2% and 4% o...

Embodiment 3

[0034] The hydrolyzed NaOH solid was adjusted to pH=6.5. When the OD value of the cultured yeast is about 2, centrifuge at 4000 r / min for 3 minutes, discard the supernatant, add pure water to shake well, centrifuge and discard the supernatant, and repeat twice. According to the inoculation amount of 150 ml of Saccharomyces cerevisiae (OD value about 2) per 1 g of algae powder, that is, 50 ml of 30 g / L algae liquid inoculates 225 ml of yeast. The hydrolyzate was added to a 100 ml airtight sample bottle and fermented at 35°C for 48 h at a speed of 150 r / min. Filter the fermentation broth through a 0.22 μm organic filter membrane, take 5ml of the filtrate and seal it in a 20ml headspace sampling bottle, and measure the ethanol content by sampling headspace gas chromatography.

[0035]30 g / L algae powder re-cultivated produced bioethanol with a concentration of 6.52 g / L and an ethanol yield of 21.7 %, while 30 g / L algae powder without re-cultured produced a bioethanol concentrati...

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Abstract

The invention discloses a method for preparing bioethanol from bloom-forming cyanobacteria. The method includes the following steps that 1, bloom-forming cyanobacteria is salvaged and collected; 2, the collected bloom-forming cyanobacteria is inoculated into a photobioreactor, the ratio of nitrogen to phosphorus is regulated, a photosynthesis promoter and a metabolic interfering agent are added, air is introduced, an inorganic carbon source is supplied, and re-culture is performed; 3, after re-culture, a tubular ultrafiltration film is used for filtering and concentration to form high-concentration cyanobacteria liquid, and cyanobacteria powder is obtained after drying; 4, the cyanobacteria powder is hydrolyzed under acidic conditions, and hydrolysate containing reducing sugar is obtained;5, the hydrolysate is adjusted to weak acidity, and yeast is added for fermentation to obtain bioethanol. According to the method, the sugar content in the bloom-forming cyanobacteria and the bio-availability are significantly improved, and the yield of the bioethanol is greatly improved. The method is simple and easy to operate, the cost is low, the problem is solved that algae needs large-scalecultivation in the existing bioethanol preparation process, and meanwhile an effective way of resource utilization is provided for bloom-forming cyanobacteria.

Description

technical field [0001] The invention belongs to the fields of bioenergy technology and environmental protection, and in particular relates to a method for preparing bioethanol by recultivating cyanobacteria in bloom. Background technique [0002] In recent decades, the problem of eutrophication in lakes has become increasingly serious, leading to the proliferation of blue-green algae and frequent occurrence of catastrophic algal blooms, posing a serious threat to lake functions and ecosystems. Salvage is an emergency removal technology to eliminate the threat of cyanobacteria blooms in a short period of time, and it is also the main method for domestic cyanobacteria bloom control. It is used regularly in eutrophic lakes in my country, such as Taihu Lake, Dianchi Lake, and Chaohu Lake. Taking Taihu Lake as an example, from 2007 to 2015, cities around Taihu Lake salvaged 8.5×10 algae water. 6 m 3 (with algae rate of 0.5%), which is equivalent to removing cyanobacteria dry ...

Claims

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Application Information

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IPC IPC(8): C12P7/08C12R1/865
CPCC12P7/08
Inventor 陈雪初黄莹莹刘思路沈应时
Owner EAST CHINA NORMAL UNIVERSITY
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