Magnetic nanoparticle and method for preparing elevated intraocular pressure animal model from same

Abstract

The invention provides a magnetic nanoparticle and a method for preparing an elevated intraocular pressure animal model from the same. The magnetic nanoparticle is adjustable in size, is provided witha multi-stage assembly structure, and can be prepared through an emulsion polymerization method. When the elevated intraocular pressure animal model is prepared, the magnetic nanoparticle is guided into eyes through anterior chamber injection, self-crosslinking or decrosslinking can be adjusted according to a specific microstructure structure of an aqueous humor circulation path, namely a trabecular meshwork / Schlemm's canal, and a stable aqueous humor blocking layer is formed. An aqueous humor moving channel is plugged in all directions and multiple levels, aqueous humor return fluid circulation is limited, the aqueous humor outflow resistance is increased, and the elevated intraocular pressure is maintained lastingly. The preparation method of the elevated intraocular pressure animal model is simple, convenient to use, safe and long in effect.

Description

technical field [0001] The invention relates to a magnetic nano particle and a method for preparing an animal model of high intraocular pressure, belonging to the technical field of nano materials. Background technique [0002] At present, the preparation methods of animal models of glaucoma ocular hypertension include peribulbar vein ligation, dexamethasone, anterior chamber gel injection, and anterior chamber magnetic bead injection. Although these methods can prepare animal models of ocular hypertension, they have their own advantages. various problems. The general feature is poor stability of intraocular pressure and low surgical success rate. The peribulbar vein ligation can stimulate the compensatory proliferation of blood vessels, bypass the ligated vessels, and achieve venous return, resulting in the failure of modeling; the dexamethasone method can cause long-term biological toxicity to many tissues in the eye, increasing the complexity of the problem, which is not...

AI Technical Summary

Problems solved by technology

Generally characterized by poor IOP stability and low surgical success rate

The peribulbar vein ligation can stimulate the compensatory proliferation of blood vessels, bypass the ligated vessels, and achieve venous return, resulting in the failure of modeling; the dexamethasone method can cause long-term biological toxicity to many tissues in the eye, which increases the complexity of the problem and is not conducive to the accuracy of a single problem. Investigation; the gel method maintains high intraocular pressure for a short period of time, and has limited role in the in-depth investigation of long-term high intraocular pressure diseases; the size of the magnetic beads selected by the anterior chamber magnetic bead injection method is about 3 μm, and can only be placed in the trabecular meshwork of the chamber angle Enrichment, cannot go deep into the internal tissue of the aqueous humor running channel, the basic properties of the magnetic beads used are not adjustable, and cannot be well adapted to the specific microscopic tissue structure, resulting in unsatisfactory stability and persistence of high intraocular pressure. The success rate of animal models of high intraocular pressure is less than one-third

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