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Natural polymer cross-linking agent and its application in the preparation of anti-calcification biological valve

A natural polymer, biological valve technology, applied in the field of biomedical engineering, to achieve the effect of multi-tissue regeneration potential, blood fat reduction, and convenient material acquisition

Active Publication Date: 2021-10-26
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the defects in the prior art, the object of the present invention is to provide a natural polymer cross-linking agent and its application in the preparation of anti-calcification bio-valve, so that by adopting the bio-heart valve material modified by the natural polymer cross-linking agent, With better mechanical properties and stability, it can significantly reduce tissue calcification, inflammation and thrombus, and reduce biological toxicity, thereby greatly extending the service life and overcoming the serious calcification and short service life of biological heart valve materials processed by traditional glutaraldehyde cross-linking methods and other defects

Method used

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  • Natural polymer cross-linking agent and its application in the preparation of anti-calcification biological valve
  • Natural polymer cross-linking agent and its application in the preparation of anti-calcification biological valve
  • Natural polymer cross-linking agent and its application in the preparation of anti-calcification biological valve

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preparation example Construction

[0032] Wherein, the preparation method of the completely decellularized bio-based material comprises steps:

[0033] S1: Obtain fresh bio-based materials, and store fresh bio-based materials in a storage transport solution at 2-10°C; the storage transport solution is PBS solution containing penicillin and streptomycin and / or PBS solution containing penicillin and streptomycin D-Hanks solution; in the PBS solution containing penicillin and streptomycin or the D-Hanks solution containing penicillin and streptomycin, the concentration of penicillin is 100U / mL, and the concentration of streptomycin is 0.1mg / mL; The base material includes, but is not limited to, one or more of porcine pericardium, bovine pericardium, small intestine, swim bladder and mesentery;

[0034] Within 4 hours, in a sterile environment, peel off the surface fat of the bio-based material, and then rinse it several times with deionized water, PBS solution and / or D-Hanks solution;

[0035] S2: The bio-based m...

Embodiment 1

[0040] This embodiment provides a kind of natural polymer cross-linking agent, and preparation method comprises steps:

[0041] Dissolve 0.4g of sodium alginate in 2mL of ultrapure water, then dilute with 38mL of PBS phosphate buffer to prepare a 10.0mg / mL sodium alginate cross-linking agent solution, and adjust the pH to 7.4;

[0042] Wherein, the formula composition of PBS phosphate buffer saline (1L) is as follows: Sodium chloride (NaCl) 8g, disodium hydrogen phosphate (NaCl) 2 HPO 4 ) 1.42g, potassium dihydrogen phosphate (KH 2 PO 4 ) 0.27g, potassium chloride (KCl) 0.2g.

Embodiment 2

[0044] This embodiment provides a kind of natural polymer cross-linking agent, and preparation method comprises steps:

[0045] Dissolve 0.4g of oxidized sodium alginate in 2mL of ultrapure water, then dilute with 38mL of PBS phosphate buffer to prepare a 10.0mg / mL oxidized sodium alginate cross-linking agent solution, and adjust the pH value to 7.4;

[0046] Wherein, the formula composition of PBS phosphate buffer saline (1L) is as follows: Sodium chloride (NaCl) 8g, disodium hydrogen phosphate (NaCl) 2 HPO 4 ) 1.42g, potassium dihydrogen phosphate (KH 2 PO 4 ) 0.27g, potassium chloride (KCl) 0.2g.

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Abstract

The invention relates to a natural polymer cross-linking agent and its application in the preparation of an anti-calcification biological valve. The natural polymer cross-linking agent is prepared by dissolving a natural polymer compound in a solvent and then diluting it with a buffer; the natural high Molecular compounds include but are not limited to one or more of alginate, oxidized alginate, chitosan, chitin, hyaluronic acid, and gelatin; the preparation method of the anti-calcification biological valve includes the steps: The bio-based material is immersed in a mixed solution of a natural polymer cross-linking agent and a catalyst for cross-linking. After the cross-linking is completed, it is fully washed and then post-treated to obtain an anti-calcification bioprosthesis. The anti-calcification biological valve provided by the present invention has better mechanical properties and stability, can significantly reduce tissue calcification, inflammation and thrombus, reduce biological toxicity, greatly prolong the service life, and overcome the biological heart valve treated by traditional glutaraldehyde crosslinking method Material calcification serious, short service life and other defects.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a natural polymer cross-linking agent and its application in preparing anti-calcification biological valves. Background technique [0002] In recent years, the incidence of heart valve diseases has continued to increase worldwide, and the total number of valve replacements is expected to increase from 290,000 in 2003 to 850,000 in 2050. In clinical practice, mechanical valves and biological valves are the main replacement options. Patients using mechanical valves need long-term anticoagulant therapy, and the chances of postoperative thrombosis and anticoagulant complications are relatively high. Therefore, they have been gradually replaced by biological valves in recent years. Bioprosthetic valves are mainly derived from acellular pericardial xenografts, acellular aortic valve xenografts, and frozen allografts. All xenografts need to undergo a series of cross-linki...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08L5/04C08L5/08A61L27/36A61L27/20
CPCA61L27/20A61L27/3625A61L27/3687A61L2400/02A61L2430/20C08J3/24C08L5/04C08L5/08
Inventor 刘婧王志红冷希岗孔德领
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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