Method for measuring contents of iron ions in different valence states in feed

A technology for iron ions and feed, which is applied in measurement devices, preparation of test samples, and measurement of color/spectral properties, etc. It can solve the problems of complicated electrode substrate preparation and modification process, unsuitable for iron ion determination, and slow color reaction speed. , to achieve the effect of strong anti-interference ability, low detection limit and high sensitivity

Inactive Publication Date: 2019-04-19
江苏澳华生物科技研究院有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

The electrochemical detection method has high sensitivity, but the preparation and modification process of the electrode substrate is relatively complicated, and an electrochemical workstation is required
Spectrophotometry has the advantages of high sensitivity, simple equipment, easy operation, and rapidity. However, spectrophotometry is currently widely used in the determination of iron in water quality, mainly including thiocyanate spectrophotometry and o-phenanthroline spectrophotometry. These detection methods have low sensitivity, slow color reaction speed, and are not suitable for the determination of iron ions in feed.
At the same time, conventional rapid analysis methods cannot measure iron ions in different valence states

Method used

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  • Method for measuring contents of iron ions in different valence states in feed
  • Method for measuring contents of iron ions in different valence states in feed
  • Method for measuring contents of iron ions in different valence states in feed

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Example 1: Detection scheme of iron ions in different valence states in feed

[0053] (1) Prepare the solution

[0054] A1 solution: phenanthrolazine total iron working solution, including 4.0mM phenanthrolazine solution, 0.1M hydroxylamine hydrochloride solution and 0.1M sodium thiosulfate solution, the buffer is acetic acid solution with ph=6;

[0055] Solution A2: ferrous phenanthrolazine working solution, including 4.0 mM phenanthrolazine solution and 0.1 M sodium thiosulfate solution, the buffer is acetic acid solution with ph=6;

[0056] Solution B: blank solution, 0.2M acetic acid buffer solution;

[0057] Solution C: iron standard solutions 0, 0.1, 0.5, 1, 2, 4, 6, 8 μg / mL, respectively C1-C8 iron standard solutions;

[0058] (2) Sample pretreatment

[0059] The feed sample was pulverized and mixed with 1% trichloroacetic acid solution at a ratio of 1:100 (g / ml). After shaking, it was filtered through quantitative filter paper or centrifuged to obtain the sup...

Embodiment 2

[0070] Embodiment 2: the drawing of working curve

[0071] Take 0, 0.1, 0.5, 1, 2, 4, 8 μg / mL of Fe 3+ Add 100 μL of the standard solution into the 96-well plate, and then add 100 μL of the total iron working solution, and measure the absorbance at 562 nm with a microplate reader after 30 min. Fig. 1 measures Fe for 5 times 3+ Regression curve for the mean of the standard series. It can be seen from the results that the correlation coefficient r is 0.9999, and the measurable concentration range is 0.1-8 μg / L. The iron content showed a linear relationship in the range of 0.1-8 μg / L, and the regression equation was y=0.1425x+0.0537.

Embodiment 3

[0072] Embodiment 3: the selection of detection wavelength

[0073] Such as figure 2 It was shown that the maximum absorption wavelength of Fe-phenanthrolazine complex is 562 nm, therefore, in order to improve detection sensitivity and reduce interference, we choose 562 nm as the optimal wavelength for the determination of iron ions.

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Abstract

The invention discloses a method for measuring contents of iron ions in different valence states in feed. The method comprises the following steps: masking copper ions by sodium thiosulfate, and reducing ferric ions in the feed to ferrous ions by hydroxylamine hydrochloride; complexing ferrous ions with phenanthroline at pH 6 and room temperature to form a purple complex with good stability; reacting for 30 minutes, and detecting at the position of 562 nm by a microplate reader; and in combination with a drawn standard curve, rapidly calculating the content of iron ions in different valence states in the feed with high throughput. Complex sample pretreatment and expensive instruments are not needed, and the method is quite suitable for being popularized and used in primary laboratories.

Description

technical field [0001] The invention relates to the field of feed quality and safety detection, in particular to a method for measuring the content of iron ions in different valence states in feed. Background technique [0002] Iron is one of the most widely distributed and common metals on the earth. It is also an essential trace element for the growth of humans, animals and plants, and participates in the metabolism of nutrients in the body. Iron deficiency can easily lead to diseases such as anemia, and excessive iron intake can increase the risk of tumors, cardiovascular and endocrine diseases. The content of iron in feed will affect the health of livestock and poultry. Excess iron will be excreted through livestock and poultry waste, which will cause iron toxicity to plants, and affect plant growth, chlorophyll content and antioxidant enzyme activity. Iron also affects the uptake of other metal ions by plants. my country stipulates that the limit standard of iron in f...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31G01N1/28G01N1/34
CPCG01N1/286G01N1/34G01N21/31G01N21/78
Inventor 陈义强赵伟许丹丹朱晓春
Owner 江苏澳华生物科技研究院有限公司
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