Method for measuring contents of iron ions in different valence states in feed
A technology for iron ions and feed, which is applied in measurement devices, preparation of test samples, and measurement of color/spectral properties, etc. It can solve the problems of complicated electrode substrate preparation and modification process, unsuitable for iron ion determination, and slow color reaction speed. , to achieve the effect of strong anti-interference ability, low detection limit and high sensitivity
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Embodiment 1
[0052] Example 1: Detection scheme of iron ions in different valence states in feed
[0053] (1) Prepare the solution
[0054] A1 solution: phenanthrolazine total iron working solution, including 4.0mM phenanthrolazine solution, 0.1M hydroxylamine hydrochloride solution and 0.1M sodium thiosulfate solution, the buffer is acetic acid solution with ph=6;
[0055] Solution A2: ferrous phenanthrolazine working solution, including 4.0 mM phenanthrolazine solution and 0.1 M sodium thiosulfate solution, the buffer is acetic acid solution with ph=6;
[0056] Solution B: blank solution, 0.2M acetic acid buffer solution;
[0057] Solution C: iron standard solutions 0, 0.1, 0.5, 1, 2, 4, 6, 8 μg / mL, respectively C1-C8 iron standard solutions;
[0058] (2) Sample pretreatment
[0059] The feed sample was pulverized and mixed with 1% trichloroacetic acid solution at a ratio of 1:100 (g / ml). After shaking, it was filtered through quantitative filter paper or centrifuged to obtain the sup...
Embodiment 2
[0070] Embodiment 2: the drawing of working curve
[0071] Take 0, 0.1, 0.5, 1, 2, 4, 8 μg / mL of Fe 3+ Add 100 μL of the standard solution into the 96-well plate, and then add 100 μL of the total iron working solution, and measure the absorbance at 562 nm with a microplate reader after 30 min. Fig. 1 measures Fe for 5 times 3+ Regression curve for the mean of the standard series. It can be seen from the results that the correlation coefficient r is 0.9999, and the measurable concentration range is 0.1-8 μg / L. The iron content showed a linear relationship in the range of 0.1-8 μg / L, and the regression equation was y=0.1425x+0.0537.
Embodiment 3
[0072] Embodiment 3: the selection of detection wavelength
[0073] Such as figure 2 It was shown that the maximum absorption wavelength of Fe-phenanthrolazine complex is 562 nm, therefore, in order to improve detection sensitivity and reduce interference, we choose 562 nm as the optimal wavelength for the determination of iron ions.
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