Preparation and detection method of fluorescent probe for detecting iodide ions in water environment

A fluorescent probe, iodide ion technology, applied in fluorescence/phosphorescence, chemical instruments and methods, measurement devices, etc., can solve complex, labor-intensive, time-consuming and other problems, and achieve good selectivity, strong signal response, and selectivity. Good results

Active Publication Date: 2019-04-23
SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are colorimetric methods, titration methods, electrochemical methods, chromatography, etc. for the detection of iodide ions. Although these methods can achieve sensitive and selective detection of iodide ions, most of these methods are complicated and time-consuming. , labor-intensive and many interference factors

Method used

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  • Preparation and detection method of fluorescent probe for detecting iodide ions in water environment
  • Preparation and detection method of fluorescent probe for detecting iodide ions in water environment
  • Preparation and detection method of fluorescent probe for detecting iodide ions in water environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of new blue-green phosphorus oxide quantum dots

[0032] Dissolve 8 mg of phosphorus pentoxide solid in 10 mL of N-methylpyrrolidone at room temperature, then add 8 mg of sodium hydroxide solid, mix and ultrasonicate for 30 minutes at room temperature, ultrasonic frequency 40KHZ, electric power 100W; The reaction solution was heated in an oil bath, and phosphorus oxide quantum dots were prepared by the solvothermal method. The temperature of the oil bath was set at 160°C, and the stirring speed was maintained at a strong speed of 360 rpm. The reaction was carried out for 8 hours; after the reaction was completed, the reaction solution was taken out The solution was centrifuged at a high speed (8000 rpm) to remove the precipitate, and the supernatant was taken; the supernatant was taken for dialysis purification, and the total time of dialysis was 2 days. The specification of the dialysis bag is 2kD. In order to make the dialysis effect good, the distille...

Embodiment 2

[0037] Prepare a phosphate buffered saline solution (PBS) with pH 6.0 in advance with deionized water for use, add 2.0 mL of buffer solution (10 mM) to a 10 mm quartz cuvette, and accurately pipette 10 μL of the pre-prepared sample obtained in Example 1 Ag + Mediated phosphorous oxide quantum dot aqueous solution (1×10 -2 mg mL -1 ) into the buffer solution in the cuvette, stirred rapidly, and put into the fluorescence meter to record the fluorescence intensity, denoted as F 0, add iodide ion (0.093mM) aqueous solution to this solution, stir for a certain period of time, until the fluorescence intensity no longer changes, record the change of fluorescence intensity under different time, such as Figure 4 As shown in (a), within 60s, the fluorescence intensity of quantum dots tends to be stable, indicating that the time period for the fluorescent probe to detect iodide ions is 1 minute, and simultaneously record different iodide ion concentrations (0.013mM, 0.021mM, 0.029 mM...

Embodiment 3

[0039] Prepare 0.02mol / L of F respectively under the conditions of Example 1 - , Cl - ,PO 4 3- ,BO 3 3- ,NO 3 2- , SO 4 2 -,NO 2 - ,Cr 2 o 7 2- (NaF, NaCl, Na 3 PO 4 , H 3 BO 3 , Na 2 SO 4 , NaNO 2 , K 2 Cr 2 o 7 ) of PBS buffer at pH 6.0, take 2 mL each and add 10 μL (1×10 -2 mg mL -1 ) of Ag + Mediated phosphorous oxide quantum dot aqueous solution, stirred and recorded fluorescence spectrum, compared with the fluorescence intensity under the action of iodide ion under the same conditions as in Example 1, obtained Figure 5 ,Such as Figure 5 (a), F - , Cl - ,PO 4 2- ,BO 3 3- ,NO 3 2- , SO 4 2 -,NO 2 - ,Cr 2 o 7 2- isoanions even at 200 times I - Under the condition of the concentration level, there is no fluorescence quenching ability, only Br- shows a little quenching ability, indicating that I - to Ag + mediated PO QDs have good fluorescence quenching ability, we also have the I - was tested with other co-existing anionic inter...

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Abstract

The invention provides a preparation method of a fluorescent probe for detecting iodide ions in a water sample. The method is characterized by comprising the following steps that 1, phosphorus pentoxide solids, sodium hydroxide solids and N-methyl-2-pyrrolidinone are mixed and are then subjected to ultrasound treatment under the room temperature condition; reaction liquid after the ultrasound treatment is put into an oil bath pot; a phosphorus oxide quantum dot is prepared by a solvothermal method; the reaction liquid is subjected to centrifugation for removing precipitates; liquid supernatantis taken; purification is performed by a dialysis method; after dialysis liquid is concentrated and freeze-dried, a blue-green fluorescent phosphoric oxide quantum dot solid material is obtained; 2,the blue-green fluorescent phosphoric oxide quantum dot solid material is dissolved into deionized water to obtain a phosphoric oxide quantum dot water solution; the phosphoric oxide quantum dot watersolution and the Ag<+> water solution are mixed; an Ag<+> mediated phosphoric oxide quantum dot probe, i.e., the fluorescent probe for detecting iodide ions in the water sample is obtained. The obtained fluorescent probe can be used for fast detecting the iodide ions; the probe has high selectivity on the iodide ions in different water samples.

Description

technical field [0001] The invention relates to a preparation method of blue-green fluorescent phosphorus oxide quantum dots and a method for detecting iodide ions, belonging to the technical field of nanomaterial preparation and application. Background technique [0002] Iodine is extremely important to animal and plant life. In advanced mammals, iodine is concentrated in the thyroid gland in the form of iodized amino acids. Iodine can promote sugar and fat metabolism, regulate water and salt metabolism, promote vitamin absorption and utilization, enhance enzyme activity, and promote growth and development. Lack of iodine can cause goiter, which seriously affects the normal development of children, manifested as mental retardation, deaf-mute, short stagnation, and growth stagnation (dwarfism). Circulatory system, digestive system, cardiovascular system and other systems, a series of hypermetabolic syndrome, hyperexcitability symptoms and eye symptoms. [0003] At present,...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/70
CPCC09K11/701G01N21/64G01N21/6428G01N21/643G01N2021/6417G01N2021/6432
Inventor 徐虎王晓梅王宇红
Owner SHANGHAI APPLIED TECHNOLOGIES COLLEGE
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