Probiotics dietary replenisher with function of alleviating type II diabetes, and application thereof

A dietary supplement, type 2 diabetes technology, applied in the food field, can solve problems such as adverse reactions, and achieve the effect of preventing damage, high content of active ingredients, and high activity

Active Publication Date: 2019-04-26
苏州微克生活科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sulfonylureas are one of the drugs for the treatment of diabetes. These drugs are effective for patients with certain islet function, but they have

Method used

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  • Probiotics dietary replenisher with function of alleviating type II diabetes, and application thereof
  • Probiotics dietary replenisher with function of alleviating type II diabetes, and application thereof
  • Probiotics dietary replenisher with function of alleviating type II diabetes, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Probiotic Dietary Supplement with the Function of Alleviating Type II Diabetes

[0055] 1. Preparation of medium: 1% peptone, 1% beef extract, 0.5 yeast powder, 2% glucose, 0.2% anhydrous sodium acetate, 0.05% magnesium sulfate heptahydrate, 0.025% manganese sulfate monohydrate, 0.2% dicitric acid Ammonium, 0.26% potassium hydrogen phosphate trihydrate, 0.1% Tween 80, add purified water and mix well, adjust the pH to 6.2-6.4, and sterilize at 121°C for 20 minutes to obtain the culture medium;

[0056] 2. Cultivation method: Lactobacillus rhamnosus strains and Lactobacillus plantarum X1 were respectively inoculated according to 1% inoculation amount based on the weight of the above-mentioned medium, and were inoculated at 37 0 Cultivate in a constant temperature incubator for 18 hours to obtain a bacterial liquid that can reach a stable period;

[0057] 3. Preparation of protective agent: Weigh 100 g of skimmed milk powder, dissolve it in 900 g of distilled w...

Embodiment 2

[0061] Embodiment 2: Acute toxicity test of dietary supplement

[0062] 1. Acute toxicity test

[0063] Take 10 3-week-old healthy male and female C57BL6 mice, and ingest 0.2 mL of dietary supplement solution once in 24 hours (take 2 g of the probiotic dietary supplement prepared in Example 1 and dissolve it in 2 ml of double-distilled water to prepare The following examples all use this solution as the formula group), observed for 14 days, and recorded the body weight, food intake and death status of the mice, and the blank group was not fed dietary supplements.

[0064] The results are shown in Table 1 and Table 2. Feeding the dietary supplements did not cause any negative effects on the mice, and the mice grew normally, without death, and without any pathological symptoms.

[0065] Table 1 Effect of feeding dietary supplements on body weight (g) of mice

[0066]

[0067] Table 2 Effect of feeding dietary supplements on food intake of mice

[0068]

Embodiment 3

[0069] Embodiment 3: feeding experiment of dietary supplement

[0070] 10 healthy male and 10 female C57BL6 mice aged 3 weeks were given 0.2 mL of dietary supplements every 24 hours, observed for 28 days, and the body weight and death status of the mice were recorded. After the experiment, all the mice were killed, the weight of the liver, kidney and spleen were weighed, and the organ coefficient was calculated; whole blood was collected for blood routine measurement; the blood was centrifuged to obtain serum, and blood biochemical tests were performed using kits (Nanjing Jiancheng Bioengineering Institute) index.

[0071] Table 3 Effect of feeding dietary supplements on body weight of mice

[0072]

[0073] Table 4 Effect of feeding dietary supplements on food intake of mice

[0074]

[0075] Routine blood test is a basic blood test that judges diseases by observing the number and shape of blood cells. The blood routine indicators of the mice were measured using an a...

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Abstract

The invention discloses a probiotics dietary replenisher with a function of alleviating type II diabetes, and application thereof. The probiotics dietary replenisher with the function of alleviating type II diabetes is prepared from freeze-drying bacteria powder, active polysaccharide and dietary prebiotics, wherein the freeze-drying bacteria powder is lactobacillus rhamnosus freeze-drying bacteria powder and lactobacillus plantarum X1 freeze-drying bacteria powder. After the bacteria are activated, the bacteria are subjected to enlargement cultivation, centrifugation and washing in sequence,and then, freeze-drying protective agent heavy suspension thalli are added to obtain thallus suspension; after the thallus suspension is pre-frozen, the thallus suspension is frozen and dried to obtain the freeze-drying bacteria powder. The dietary replenisher disclosed by the invention has a high active ingredient content, the viable bacteria content of the probiotics can be 1,011 CFU/g or above,the blood sugar level of a mouse can be lowered, the contents of glycosylated hemoglobin and endotoxin can be obviously lowered, the levels of insulin and hepatic glycogen are improved, and the probiotics dietary replenisher has a wide application prospect.

Description

technical field [0001] The invention relates to a probiotic dietary supplement with the function of alleviating type 2 diabetes and an application thereof, which is a probiotic dietary supplement with a hypoglycemic function and belongs to the technical field of food. Background technique [0002] Diabetes mellitus is a chronic metabolic disease characterized by dysfunction of the body caused by hyperglycemia. There are three pathogenesis of diabetes: 1. Insulin secretion defect; 2. Insulin action disorder, that is, insulin hyposensitivity. Insulin disorder is the decrease in the affinity of insulin to insulin receptors, resulting in insufficient insulin to promote the metabolism and absorption of sugar, causing abnormal blood sugar. 3. Islet β-cell damage, which is mainly divided into insulin-dependent type Ⅰ diabetes and non-insulin-dependent type Ⅱ diabetes, other types and four types of gestational diabetes, of which type Ⅱ diabetes patients account for about 90%. Globa...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23L33/125C12R1/225
CPCA61P3/10A23L33/125A23L33/135A23L33/21A61K31/702A61K31/715A61K31/722A61K31/733A61K35/747C12N1/20A23V2002/00A23V2400/175A23V2400/169A23V2200/328A23V2200/30A23V2250/511A23V2250/28A23V2250/5062A23V2250/51A23V2250/5116A61K2300/00
Inventor 黄洁赵建新翟齐啸崔树茂于雷雷郭仁妹王健
Owner 苏州微克生活科技有限公司
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