Distributed systems for the efficient production and use of microbe-based compositions

A microbial source and microbial technology, applied in the direction of bioreactor/fermenter combination, drug combination, biomass pretreatment, etc., can solve problems such as damage, achieve the effect of reducing production scale, increasing production, reducing inventory and distribution

Pending Publication Date: 2019-04-26
LOCUS IP CO LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Currently, agroforestry operations are limited to sourcing microbial amendments from distant producers whose product quality is affected by delays in upstream processing, methods used to stabilize product for future distribution, supply chain bottlenecks, improper storage, and other factors that prevent timely delivery and application of live, high cell count microbial products

Method used

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  • Distributed systems for the efficient production and use of microbe-based compositions

Examples

Experimental program
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preparation example Construction

[0094] Preparation of products of microbial origin

[0095] A product of microbial origin according to the invention is simply a fermented nutrient solution containing the microorganisms and / or microbial metabolites produced by the microorganisms and / or any residual nutrients. The fermented product can be used directly without extraction or purification. Extraction and purification, if desired, are readily accomplished using standard extraction methods or techniques described in the literature.

[0096] Microorganisms in products of microbial origin may be in viable or inactive form. Products of microbial origin can be used without further stabilization, preservation and storage. Advantageously, the direct utilization of these microbially derived products maintains high microbial viability, reduces the possibility of contamination from foreign agents and undesired microorganisms, and maintains the viability of by-products of microbial growth.

[0097] Microorganisms and / or ...

Embodiment 1

[0210] Example 1 : Fermentation of Candida globosa for sophorolipid (SLP) production in a 110 L dispensable reactor.

[0211] A portable, airlift, fully enclosed reactor operated by PLC with water filtration, temperature control unit and ventilator on panel was used. The reactor had a working volume of 90 L when growing S. bombicola for SLP production.

[0212] In a preferred embodiment, the nutrients used for SLP production are glucose, urea, yeast extract, canola oil, magnesium sulfate and potassium phosphate.

[0213] Reactors were inoculated with 8 liters of liquid culture grown in flasks. The duration of the culture period for SLP production was 7-8 days at 25 °C and pH 3.5, with sampling performed twice a day.

[0214] The final concentration of SLP is roughly 10% of the working volume, in this case about 9 L of product, which contains 300-400 grams of SLP per liter.

Embodiment 2

[0215] Example 2: Fermentation of Wickhamia and / or Pichia pastoris for sophorolipid production in a 450L dispensable reactor.

[0216]A portable airlift reactor operated by PLC with water filtration, temperature control unit and ventilator for adequate ventilation was used. The method can be performed as a batch culture process. When growing Wickhamia or Pichia pastoris for SLP production, the reactor has a working volume of 400 L.

[0217] In a preferred embodiment, the nutrients used for SLP production are glucose, urea, yeast extract, canola oil, magnesium sulfate and potassium phosphate.

[0218] Inoculation of this reactor required up to 5% liquid seed culture of the working volume. At a temperature of 25° C. and pH 3.5, the duration of the culture period was 7 days, with sampling performed twice a day.

[0219] The final concentration of SLP was approximately 20-25% of the working volume, in which case greater than 90 L of product was formed.

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Abstract

The invention relates to systems and methods for effective production and use of microorganisms and / or the fermentation broth in which they are produced. Advantageously, the system is cost-effective,scalable, quick, versatile, efficacious, and helpful in reducing resistance to chemical compounds and residue that concerns consumers.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application No. 62 / 385,057, filed September 8, 2016, which is incorporated by reference into this application in its entirety. Background technique [0003] Worldwide, agriculture, forestry, animal husbandry, aquaculture and other means of producing food, nutritional supplements, flavours, fibers and natural structural materials are increasingly difficult due to environmental challenges, including the resistance of pests to conventional chemicals Pesticide resistance, temperature extremes, droughts, floods, new plant pathogens and pests, transboundary breeding of pests, consumer preferences for low chemical residues and non-GMO foods, and in order to limit conventional chemical inputs such as herbicides, Various government regulations restrict the widespread use of chemicals and fertilizers due to their severe impact on the environment. The severity and unpredictabil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34A01N63/00C05F11/08C07K17/00
CPCC12M23/44C12M23/52C12M23/58C12M41/12C12P19/44C12N1/14C12N1/16C12N1/20C12P7/64C12M41/26A61P43/00Y02A40/10C05F11/08C12M41/42A01N63/32
Inventor 肖恩·法默保罗·S.·左恩肯·阿里贝克肯特·安达姆泰勒·迪克森
Owner LOCUS IP CO LLC
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