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A method, product and application of preparing nano-silver particles using emblica extract

A technology of nano-silver particles and extracts, which is applied in the field of green biosynthetic nano-materials, can solve the problems of poor antibacterial effect of nano-silver antibacterial agents, environmental pollution by chemical pesticides, and harm of bacterial drug resistance, etc., and achieve strong bactericidal effect, Good control effect, simple and easy application

Active Publication Date: 2020-09-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the antibacterial effect of the nano-silver bacteriostatic agent obtained by the above method is relatively poor, and the plant species that can be used to synthesize nano-silver particles disclosed in the prior art are less. new method, and can improve the antibacterial effect of the obtained product
[0007] In addition, studies have shown that the phenomenon of rice seeds carrying bacterial brown streak bacteria is very common, which can easily cause huge losses to my country's agricultural economy, while traditional chemical pesticides are likely to cause environmental pollution, bacterial drug resistance, and potential harm to the human body. Therefore, it is also very It is necessary to develop a new green method for preventing and treating bacterial brown streak disease in rice

Method used

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  • A method, product and application of preparing nano-silver particles using emblica extract
  • A method, product and application of preparing nano-silver particles using emblica extract
  • A method, product and application of preparing nano-silver particles using emblica extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Wash, dry and chop fresh emblica, weigh 10g of emblica and mix with 100ml of deionized water, squeeze it into juice with a juicer with a power of 150W, and perform double filtration through cotton cloth and filter paper , to ensure that the solid impurities are removed, so as to obtain the emblica extract;

[0040] (2) Take 15ml of the emblica extract obtained in step (1) and 100ml of the concentration of 1mM AgNO 3 The solutions were mixed and heated at 60°C for 20 minutes. After standing overnight, the color changed from light yellow to dark brown. Centrifuged at 10,000 rpm for 10 minutes, the precipitate was taken for washing, and vacuum freeze-dried to obtain powdered AgNPs.

[0041] figure 1 (A) is the change diagram of the color of the AgNPs solution synthesized by using the emblica extract in Example 1, and it is found that the addition of AgNO 3 After solution, the color changed from the initial light yellow to dark brown.

[0042] figure 1 (B) The ultravi...

Embodiment 2

[0047] (1) After washing, drying and chopping the fresh emblica, weigh 10g of emblica and mix it with 100ml of deionized water. Use a juicer with a power of 200W to make it into a liquid, and filter it through cotton cloth and filter paper. Double filtration to ensure that the solid impurities are removed to obtain plant extracts;

[0048] (2) Take 2.5ml of the emblica extract obtained in step (1) and 100ml of the concentration of 1mM AgNO 3 The solutions were mixed and heated continuously at 65°C for 40 min. After standing overnight, the color changed from light yellow to dark brown. Centrifuged at 8000 rpm for 10 min, the precipitate was collected for washing and vacuum freeze-dried to obtain powdered AgNPs.

Embodiment 3

[0050] (1) After washing, drying and chopping the fresh emblica, weigh 10g of emblica and mix it with 100ml of deionized water. Use a juicer with a power of 400W to make it into a liquid, and filter it through cotton cloth and filter paper. Double filtration to ensure that the solid impurities are removed to obtain plant extracts;

[0051] (2) Take 10ml of the emblica extract obtained in step (1) and 100ml of the concentration of 1mM AgNO 3 The solutions were mixed and heated at 70°C for 30 minutes. After standing overnight, the color changed from light yellow to dark brown. The solution was centrifuged at 9000 rpm for 15 minutes, and the precipitate was washed and vacuum freeze-dried to obtain powdered AgNPs.

[0052] Antibacterial effect evaluation:

[0053] (1) Effect of AgNPs concentration on antibacterial effect

[0054] In order to evaluate the activity of AgNPs in inhibiting the brown streak pathogen of rice, the AgNPs powder prepared in Example 1 was prepared into so...

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Abstract

The invention discloses a method for preparing nano-silver particles through a phyllanthus emblica extracting solution, the product and application of the nano-silver particles. The preparation methodincludes the following steps that 1), phyllanthus emblica is completely washed, dried and cut up and then mixed with deionized water, the mixture is squeezed into juice, and the phyllanthus emblica extracting solution is obtained after the juice is fully filtered; and 2), the phyllanthus emblica extracting solution and a AgNO3 solution are mixed for a reaction, obtained mixed liquid is centrifuged, washed and subjected to vacuum freeze drying, and the nano-silver particles are obtained. The obtained nano-silver particles have small average grain sizes and stable structures, can effectively restrain the activity of rice bacterial brown streak bacteria, and has an extremely high sterilization effect; and the nano-silver particles serve as an agricultural antibacterial agent to be applied topreventing and controlling rice bacterial brown streak, is environmentally friendly and effective, has no potential danger to the human body, and has broad application and popularization prospects inthe agricultural production field.

Description

technical field [0001] The invention belongs to the technical field of green biosynthetic nanomaterials, and in particular relates to a method for preparing nano-silver particles using emblica extract and its application in agriculture. Background technique [0002] Due to its unique physical and chemical properties, strong antibacterial ability and no drug resistance, nano silver is widely used in chemical industry, sensing, food packaging, medicine, environmental technology and other fields, and has great market development potential. Among them, the strong antibacterial performance is due to its small particle size and large specific surface area. It has been reported very early that Ag has potential antibacterial activity and can inhibit Escherichia coli and Staphylococcus aureus. [0003] At present, the traditional methods of synthesizing nano-silver mainly include physical methods, chemical methods, and microbial methods. The use of physical methods and chemical metho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24A01G13/00A01G22/22
Inventor 李斌洪纤纤姚榕刘梦菊邱文张阳马媛媛马宋
Owner ZHEJIANG UNIV