A kind of organosilicon polymer fluorescent probe for detecting thiocyanate and its synthesis and application
A fluorescent probe, thiocyanate technology, used in fluorescence/phosphorescence, luminescent materials, material excitation analysis, etc., can solve the problems of low sensitivity, high biological toxicity of the probe, and poor water solubility of the probe
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Embodiment 1
[0041]Example 1 Synthesis of Compound Probe V1
[0042](1) Synthesis of Compound A
[0043]
[0044]The 2.3 g of bisphenolfluorene is first soluble in 20 mL of ethanol, and then 0.75 g of polymethylene formaldehyde is dissolved in 20 ml of toluene. Add mm in 250ml smear flaskNH2 2 g, dissolved in 100 mL of ethanol, then adding a modified formaldehyde solution to a bisphenol fluore solution, heating at 110 ° C and stirring reflow 24 h. Steam, dried to give compound A.
[0045](2) Synthesis of Compound B
[0046]
[0047]2.77 g 4-bromine-1,8-naphthane was dissolved in 50 ml of ethanol, and then 3 g of sodium methoxide was dissolved in 50 ml of ethanol, and another 250 ml of the solution of the solution was added, and the reaction was stirred at room temperature. 24 h. After the reaction, 100 ml of water was added to the reaction bottle to give a yellow precipitate. After filtration, the column chromatography was purified, the eluate was methanol: dichloromethane (v / v) = 1: 10, obtained Pure compound...
Embodiment 2
[0051]Example 2 Compound V1 fluorescence selection for selectivity of different anions
[0052]Preparation of 5 ml of sodium chloride, sodium acetate, sodium sulfide, sodium bicarbonate, sodium sodium sodium sodium sodium sodium sodium sodium sodium sodium sulfate, sodium nitrate, sodium sodium phosphate, sodium sulfate (pH = 7.4) Example 1 of the concentration of 1 mm The fluorescent probe V1 obtained was used as a spare.
[0053]Add 10 μL of probe, 225 μL of DMSO and 10 equivalents of sodium chloride, sodium acetate, sodium sodium hydrogencarbonate, sodium thiocyanate, sodium hypochlorite, sodium nitrite, sodium nitrate, sodium phosphate, sodium sulfate, sodium carbonate. Sodium solution, configure to 3 mL, shake, 30 min, perform fluorescence detection (λ) with phosphate buffer PBS to 3 mL, shake, 30 minEX = 405 nm, λEM= 530 nm), establish fluorescence intensity column chart after adding each ionic solution, such asfigure 2 Indicated. byfigure 2 It can be found that other ions have a sm...
Embodiment 3
[0054]Example 3 Fluorescence Test Detection of Different Concentrations of Thiocyanate on Probe V1
[0055]The fluorescent probe V1 obtained by preparing a 10 mL concentration of 10 mm of thiocyanate and a concentration of 1 mm 1 mm was prepared as a spare.
[0056]The formulated probe concentration is 10 μm, which interacts with different concentrations of thiocyanate (5, 10, 10, 20, 70, 80, 10, 140 μm), and carried out Fluorescence detection (λEX = 405 nm, λEM= 530 nm), calculating fluorescence intensity in each system, establishing fluorescence intensity and thiocyanate concentration standard curve. Such asimage 3 As shown, with the increase of thiocyanate concentration, the fluorescence intensity of the reaction system gradually decreases, and when the thiocyanate concentration reaches 100 μm, the fluorescence intensity of the reaction system reaches the lowest state.
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