Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for measuring content of trans-resveratrol in dried peanut seeds

A technology of resveratrol and dry seeds is applied in the field of detection of active substances in food, which can solve the problems of inconspicuous peaks, complex components, and many impurities of the target, achieve high selectivity, accurate and reliable content, and improve detection. The effect of efficiency

Inactive Publication Date: 2019-05-03
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition and many impurities in peanut seeds, the peak of the target object is not obvious, and the interference is large

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring content of trans-resveratrol in dried peanut seeds
  • Method for measuring content of trans-resveratrol in dried peanut seeds
  • Method for measuring content of trans-resveratrol in dried peanut seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Chromatographic conditions: US Agilent 1290Infinity II high performance liquid chromatograph, ChemstationEdition chromatographic workstation:

[0055] Column: Agilent HC-C18 column

[0056] Column temperature: 30°C;

[0057] Detection wavelength: 306nm;

[0058] Mobile phase: elution time is 0-16min, acetonitrile-water (22:78); elution time is 16-18min, acetonitrile-water (40:60);

[0059] Flow rate: 0.6ml / min;

[0060] Injection volume: 10ul.

[0061] Establishment of the t-RVT standard curve: Using the external standard method, weigh an appropriate amount of t-RVT standard products, dissolve and constant volume with methanol, and prepare the t-RVT standard products at concentrations of 0.25, 0.50, 0.75, 1.00, 1.50, 2.00, 2.50, 3.00, 3.50, 4.00mg / l, pass the prepared solution through a 0.22um filter membrane, put it into a sample bottle for determination. The obtained data takes the peak area as the ordinate and the concentration as the abscissa to obtain the peak...

Embodiment 2

[0067] Single factor experiment of t-RVT pretreatment method in dried peanut seeds.

[0068] 1. The effect of the concentration of enzymatic hydrolyzate on the yield of peanut t-RVT.

[0069] The concentration of the enzymolysis solution was set to 0, 2, 4, 6, 8g / l respectively, and 3.00g of peanut powder was accurately weighed, and the pretreatment was carried out under the condition that the enzymolysis time was 2h and no ultrasonic treatment was performed. Three repetitions were set up for each group.

[0070] The result is as Figure 4 As shown, the concentration of the enzymolysis solution is in the range of 0-4g / L, the yield of t-RVT increases with the increase of the concentration of the enzymolysis solution. When the concentration of the enzymolysis solution increases to 4g / L, the t-RVT - RVT yield rate has not changed much. Therefore, it is better to choose the concentration of the enzymatic hydrolyzate to be about 4g / L.

[0071] 2. The effect of enzymatic hydroly...

Embodiment 3

[0089] The present embodiment provides a method for determining the content of resveratrol in dried peanut seeds using a high-performance liquid chromatography analysis method, including the following steps:

[0090] (1) Select dry peanut seeds with uniform size, consistent color, and full maturity, and use a small grinder to crush the selected dried peanut seeds into fine, uniform powder (between 30 mesh and 40 mesh), and use a thousand points One balance accurately weighs 3.00 g of peanut powder (the error range is controlled within ±0.05%) in a 50 ml centrifuge tube.

[0091] (2) Add 5ml of enzymatic hydrolysis solution, put the lid on and put it into a constant temperature water bath at 50°C for enzymatic hydrolysis, and control the enzymatic hydrolysis time to 120min.

[0092] (3) Add 12ml of absolute ethanol, mix well, put into a small-scale ultrasonic cleaning machine with a frequency of 40kHz for 20min, centrifuge at 8000rpm, take 10ml of supernatant to obtain the crud...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for measuring the content of trans-resveratrol in dried peanut seeds. The method comprises the steps: placing peanut dry seed powder in enzymatic hydrolysate and carrying out enzymolysis, adding ethanol, carrying out mixing uniformly, carrying out ultrasonic processing for 10min to 4h at the frequency of 30 to 60kHz, carrying out centrifugation and taking a supernatant to obtain crude extract of the peanut dried seeds; and measuring the content of the trans-resveratrol in the crude extract of the peanut dried seeds by using a high-efficiency liquid chromatogram.The enzymatic hydrolysate is a cellulose aqueous solution with the concentration of 1 to 8g / L. According to the provided measuring method, the pretreatment method is optimized; and compared with theexisting method, the provided measuring method enables the time and solvent to be saved. The measuring method having advantages of high accuracy and repeatability is an analysis method with characteristics of rapidness, high accuracy, and high sensitivity; and the reference to extract the t-RVT process from peanuts and measure the t-RVT content in the dried peanut seeds is provided.

Description

technical field [0001] The invention relates to the field of detection of active substances in food, in particular to a method for measuring the content of trans-resveratrol in dried peanut seeds. Background technique [0002] Resveratrol (Resveratrol), also known as stilberol, is a non-flavonoid polyphenol compound, which is a white needle-like crystal with a molecular formula of C 14 h 12 o 3 The structural formula is: [0003] [0004] Natural resveratrol has two structures, cis and trans, which can be combined with glucose to form cis or trans resveratrol glycosides. [0005] Resveratrol glycosides can release resveratrol under the action of glycosidase in the intestine, and the physiological activity of the trans isomer is stronger than that of the cis, and the activity of the monomer is greater than that of the glycoside. Resveratrol widely exists in Polygonum cuspidatum, peanuts, grapes and other plants. With the deepening of research, its pharmacological effec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
Inventor 徐思亮罗怀勇陈海文喻博伦郭建斌陈伟刚赵姣姣
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com