A kind of marine microbial flocculant and preparation method thereof
A microbial flocculant and marine microorganism technology, which is applied in the field of microbial flocculant preparation, can solve the problems of complex composition of microbial culture medium, difficult preparation, and insufficient application range, and achieves wide application range, few production process steps, and culture conditions. less demanding effects
Active Publication Date: 2021-10-15
山东大湖新材料有限公司
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Problems solved by technology
In recent years, the research on microbial flocculants has been paid more and more attention, but the microbial flocculants currently used have various deficiencies, such as the flocculant disclosed by the patent number CN101121920A, which can only handle wastewater with a pH of 6-7, and the scope of use is not wide enough; and The composition of microbial culture medium is complex and difficult to prepare
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[0024] A preparation method of marine microbial flocculant, the preparation steps are as follows:
[0025] Step 1. Weigh 45g of starch, 2.5g of ammonium sulfate, 20g of sodium chloride, 2.9g of magnesium sulfate, 4.5g of magnesium chloride, 0.64g of potassium chloride, and 1.75g of calcium chloride, and add them to 1000mL of water, adjust the pH to 6.5, at 112°C Moist heat sterilization for 30 minutes;
[0026] Step 2. Inoculate Amylobacter amylobacterium into a conical flask containing 1000mL liquid medium at a volume ratio of 1:100, and cultivate for 24 hours at a temperature of 25°C and a shaker speed of 150r / min;
[0027] Step 3, centrifuging the culture solution obtained in step 2 at 5000r / min for 30min, removing the precipitate, and the supernatant is the marine microbial flocculant;
[0028] Step 4. Add twice the volume of absolute ethanol to the supernatant obtained in step 3, let it stand at 2-6°C for 12 hours, centrifuge at 9000r / min for 20min, collect the precipi...
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The invention provides a marine microbial flocculant produced by fermentation of an amylbacteria (Amylibacter lutimaris), which has a flocculation effect in a wide range of pH and high salt concentration, and has a wide range of applications. The invention provides a method for preparing a marine microbial flocculant. The preparation process of the liquid medium is simple, and the sources of various components are widely and easily obtained. The requirements for microbial culture conditions are low, and the culture time is short. After inoculation, the biological flocculant can be obtained only by fermentation. , less production process steps, precipitation, centrifugation, drying to obtain dry flocculant products, purification process is simple to operate, the obtained dry product flocculant has the advantages of stable storage and convenient use.
Description
technical field [0001] The invention relates to a method for preparing a microbial flocculant, in particular to a method for preparing a biological flocculant by using amylobacter. Background technique [0002] Flocculation and sedimentation method plays an important role in water supply and sewage treatment. At present, the flocculants widely used in water treatment mainly include inorganic polymer flocculants represented by aluminum series and organic polymer flocculants represented by polyacrylamide. Inorganic flocculants are generally used in large amounts, and non-degradable inorganic ions have certain toxicity, which will seriously pollute the ecological environment and endanger human health; while organic polymer flocculants (such as: polyacrylamide) polymers are not toxic, but Its residues are likely to cause secondary pollution to the ecological environment, and its monomer acrylamide has strong neurotoxicity and is a strong carcinogen. The United States, Britain a...
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IPC IPC(8): C02F1/52C02F1/54C12P1/04C12R1/01
Inventor 贾保磊邵明伟田差沃
Owner 山东大湖新材料有限公司