High performance liquid chromatography detection method of free methoxy group noradrenaline and methoxy group in human plasma

A technique for metanephrine and norepinephrine, applied in the field of analysis and detection, can solve the problems of complex pretreatment process, poor method specificity and specificity, high detection difficulty and cost, and achieve simplified pretreatment extraction and derivatization. The operation process and the pre-processing process are simple and the effect of simplifying the pre-processing process
CN109725079APending Publication Date: 2019-05-07杭州凯莱谱精准医疗检测技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
杭州凯莱谱精准医疗检测技术有限公司
Publication Date
2019-05-07

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Abstract

The invention discloses a high performance liquid chromatography detection method of free methoxy group noradrenaline and methoxy group in human plasma. The high performance liquid chromatography detection method of the free methoxy group noradrenaline and the methoxy group in the human plasma comprises the following steps that: (1) sample pre-treatment is conducted, interior label working solution and spiritus mindereri are successively added into a plasma sample, vortex is mixed, and centrifugation is conducted; (2) weak positive ion exchange solid phase extraction 96 pore plate (SPE 96 poreplate) is activated; (3) solid phase extraction treatment is conducted, after the step (1) is conducted, the sample is loaded into the treated SPE 96 pore plate, a formic acid acetonitrile solution is eluted, and eluent is dried by nitrogen; and (4) redissolution is conducted, analysis and detection are conducted by the high performance liquid chromatography detection method. The high performanceliquid chromatography detection method of the free methoxy group noradrenaline and the methoxy group in the human plasma has the advantages that accuracy is high, reproducibility is high, sensitivityis high, specificity is high, the efficiency is high, flux is high, the cost is low, and the operation is easy, convenient and fast.
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Description

technical field

[0001] The invention relates to the technical field of analysis and detection, in particular to a high-accuracy, high-reproducibility, high-sensitivity, high-specificity, high-efficiency, high-throughput, low-cost, simple and fast operation method for removing free methoxy in human plasma. High performance liquid chromatography tandem mass spectrometry detection method for norepinephrine and methoxyepinephrine. Background technique

[0002] Catecholamines are amine compounds with catechol (ie catechol) structure, including dopamine, norepinephrine, epinephrine and their derivatives. Norepinephrine and epinephrine are both hormones secreted by the adrenal medulla and neuromediators of noradrenergic fibers in the sympathetic and central nervous systems. Noradrenaline is widely distributed in the central nervous system, and its content is relatively large, while that of epinephrine is relatively small. Dopamine is mainly concentrated in the extrapyramidal syst...

Claims

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