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Fermentation method of utilizing sugar refining tailing and tryptophan mother liquid to produce biological feed

A tryptophan mother liquor and bio-feed technology, applied in the field of microbial fermented feed, can solve the problems of aggravated agricultural production shrinkage, source of raw materials for feed enterprise production, insufficient domestic grain production and supply, and low added value of agricultural products, so as to improve biological utilization efficiency, reduced breeding costs, improved color and palatability

Pending Publication Date: 2019-05-24
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the domestic environmental protection situation is severe. The primary problem that must be solved for the sustainable development of the biopharmaceutical industry is environmental protection. However, the investment in environmental protection is huge, and the added value of the agricultural products produced is low, resulting in insufficient funds for enterprise development.
On the other hand, the market price of agricultural products is low, resulting in insufficient domestic food production and supply, further exacerbating the shrinkage of agricultural production and the shortage of raw material sources for feed enterprises and enterprises.
Thirdly, in the biopharmaceutical or feed industry, the added value of agricultural products is disclosed, resulting in a lot of waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] ② Preparation of yeast strains:

[0034] Take out the strain preservation tube, draw a plate with PDA solid medium for recovery and expansion, and incubate at 36°C for 12h. Pick a loop under the plate and inoculate it into 50ml of PDA medium, incubate in an incubator at 36°C and 180rpm for 12~15h. Inoculate 1000ml of seed culture medium with 5% inoculum amount, incubate in an incubator at 36°C and 180rpm for 12-15 hours, and test the concentration of the bacteria solution. If the bacteria content exceeds 100 million CFU / ml, it can be used as a seed inoculation;

[0035] PDA medium: 200g potatoes, 20g sucrose, 2% agar was added to the solid medium;

[0036] Yeast seed medium: tryptophan mother liquor 15g / L, corn steep liquor 15g / L, ammonium sulfate 2g / L, corn bran 10g / L, sugar residue 10g / L, sodium bicarbonate 0.5g / L, pH7.0~7.5 .

Embodiment 1

[0038] Raw materials by weight include: 25% corn steep liquor, 25% corn bran, 2% ammonium sulfate, 21% sugar residue, 1% sodium bicarbonate, 10% tryptophan mother liquor, 8% Bacillus subtilis, 8% yeast .

[0039] Specific steps:

[0040] (1) Add 10% tryptophan mother liquor, 25% corn steep liquor, and 2% ammonium sulfate to the mixing tank in sequence, adjust the pH to 7.5~8.0 with liquid caustic soda, then add 25% corn bran and 21% sugar residue in sequence , sodium bicarbonate 1%, after stirring evenly with a mixer, the materials are evenly separated, and 8% of Bacillus subtilis and 8% of yeast are added respectively to carry out separate pure culture;

[0041] (2) Spread evenly stirred pure culture materials on trays or fermentation tanks, in a fermentation room with a humidity of 55%~75% and a temperature of 30~40°C, ferment for 24~36 hours, turn the materials in the middle, and carry out concurrent fermentation. Sexual anaerobic fermentation, the fermented material prod...

Embodiment 2

[0045] The proportion of raw materials by weight includes: corn steep liquor 30%, corn bran 20%, ammonium sulfate 2%, sugar residue 21%, sodium bicarbonate 1%, tryptophan mother liquor 10%, Bacillus subtilis 8%, yeast 8% .

[0046] Specific steps:

[0047] (1) Add 10% tryptophan mother liquor, 30% corn steep liquor, and 22% ammonium sulfate to the mixing tank in turn, adjust the pH to 7.5~8.0 with liquid caustic soda, then add 20% corn bran and 21% sugar residue in turn , sodium bicarbonate 1%, after stirring evenly with a mixer, the materials are evenly separated, and 8% of Bacillus subtilis and 8% of yeast are added respectively to carry out separate pure culture;

[0048] (2) Put the homogeneously stirred pure culture material directly into a breathable inner film bag, and ferment for 24~36 hours in a fermentation room with a humidity of 55%~65% and a temperature of 30~35°C, and turn over the material in the middle to carry out Facultative anaerobic fermentation, the ferm...

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Abstract

The invention provides a fermentation method of utilizing sugar refining tailing and tryptophan mother liquid to produce biological feed. The fermentation method includes: sequentially adding tryptophan mother liquid, corn pulp and ammonium sulfate into a mixing tank, using alkali liquid to adjust pH of fermentation raw materials, sequentially adding corn bran, pond sediment and sodium hydrogen carbonate, stirring well in the mixing tank, dividing a material mixture into two parts, respectively adding bacillus subtilis and yeast into the two parts of the material mixture, respectively performing facultative anaerobic fermentation on two kinds of the material mixtures added with different zymophytes, mixing after fermentation is completed, performing anaerobic fermentation again, directly bagging and sealing a re-fermentation material mixture after fermentation is finished, or smashing and packaging after drying. By the fermentation method, the problem of downstream treatment difficultyof the sugar refining tailing and the tryptophan mother liquid in the process of tryptophan production is solved, and environment protection pressure of amino acid production is relieved.

Description

technical field [0001] The invention relates to a microbial fermented feed, in particular to a fermentation method for producing biological feed by using sugar-making tailings and tryptophan mother liquor. Background technique [0002] At present, the domestic environmental protection situation is severe. The primary problem that must be solved for the sustainable development of the biopharmaceutical industry is environmental protection. However, the investment in environmental protection is huge, and the added value of the agricultural products produced is low, resulting in insufficient funds for enterprise development. On the other hand, the market price of agricultural products is low, resulting in insufficient domestic grain production and supply, which further exacerbates the shrinkage of agricultural production and the shortage of raw material sources for feed enterprises and as long as enterprises produce. Thirdly, in the biopharmaceutical or feed industry, the added ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/33A23K20/142A23K10/18A23K20/22A23K10/30A23K10/37A23K20/20
CPCY02P60/87
Inventor 王卫富马文艳李利娜魏志娟郭端阳
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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