Peony cavity spore leaf spot pathogen separation and selection culture medium and application thereof

Abstract

The invention belongs to the field of selection and separation of plant pathogenic fungi, and particularly relates to a peony cavity spore leaf spot pathogen separation and selection culture medium and an application thereof. The peony cavity spore leaf spot pathogen separation and selection culture medium comprises a basal culture medium, streptomycin sulfate with final concentration of 50-100mg / L, mancozeb with final concentration of 3.125-12.5mg / L and carbendazim with final concentration of 3.125-12.5mg / L, and the basal culture medium is a potato sucrose agar culture medium. The peony cavity spore leaf spot pathogen separation and selection culture medium is used for separating pathogens of peony cavity spore leaf spot strains and has the advantages that the culture medium is simple tooperate and can be operated under a non-sterile condition, time and labor are saved, requests for testers are low and the like.

Description

technical field [0001] The invention belongs to the field of screening and isolation of plant pathogenic fungi, and in particular relates to a medium for isolation and screening of coelomia coelomosa leaf spot fungus and application thereof. Background technique [0002] In the research work of pathogenic fungi, pure cultures of pathogenic fungi are often required. However, under natural conditions, pathogenic bacteria are usually mixed with other miscellaneous bacteria, and pathogenic bacteria need to be isolated from diseased tissues or other substrates. The isolation of plant pathogenic fungi plays a very important role in the scientific research of plant pathology. [0003] In recent years, in the process of peony cultivation and management, due to factors such as lax disinfection of soil and seedlings, improper cultivation methods, poor management, and climate, the harm of fungal diseases has been increasing year by year, and several new diseases have appeared. Leaf s...

AI Technical Summary

Problems solved by technology

[0005] (1) Since a sample needs to be processed through four steps of leaf segmentation and cutting, repeated disinfection, water washing and filter paper drying, the sample processing is more troublesome and laborious;

[0006] (2) There are many reagents and consumables required, and six petri dishes are needed in the process of disinfection and cleaning. If there are many diseased plants, more petri dishes are needed, and more alcohol and NaClO need to be purchased, so the cost Higher, if mercury chloride is used, it is easy to pollute the environment and is not safe for operators;

[0007] (3) Due to the cumbersome operation process, it is very time-consuming. Generally, each diseased plant needs to be treated for 10-15 minutes before the isolated material can be cultivated;

[0008] (4) Strict requirements for the test operation. The separation material should be as fresh as possible. The disinfection time is not easy to grasp, and it should not be too long or too short. If the hyphae of germs are too long, they will be easily killed, and if the disinfection is too short, it will easily breed miscellaneous bacteria;

[0009] (5) Vulnerable to bacterial contamination during the separation process

[0010] (6) Strict requirements for aseptic operation: the tissue separation method requires that the test materials must be brought back to the laboratory and separated on an ultra-clean workbench, which is impossible for field investigations

Obviously, the tissue separation method cannot meet this requirement

[0011] Due to the shortcomings of time-consuming, laborious, high cost, high operation requirements, easy to be contaminated by miscellaneous bacteria and inability to carry out under natural conditions when using the tissue separation method to isolate coelomosa leaf spot, it is necessary to isolate coelomosa leaf spot. Simple, rapid and non-sterile isolation method research

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