Bone scaffold material as well as preparation method and application thereof

A technology of bone scaffold and composite material, which is applied in the field of high-strength bone scaffold material and its preparation, can solve the problems of high preparation cost and low compressive strength, and achieves the effects of short time consumption, low requirements, and favorable cell adhesion.

Inactive Publication Date: 2019-05-28
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to provide high-strength bone scaffold material and its preparation method and application in order to overcome the low compressive strength of bone scaffold materials in the prior art, mostly below 60MPa, containing organic solvents, and high production cost.

Method used

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  • Bone scaffold material as well as preparation method and application thereof
  • Bone scaffold material as well as preparation method and application thereof
  • Bone scaffold material as well as preparation method and application thereof

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preparation example Construction

[0036] A first aspect of the present invention provides a method for preparing a bone scaffold material, wherein the method comprises the following steps:

[0037] (A) 3D direct-writing printing of biocompatible materials to make a bone scaffold structure;

[0038] (B) forming, drying and modifying the obtained bone scaffold structure to obtain a bone scaffold material,

[0039] Wherein, the biocompatible material, based on the total weight of the material, contains 12-26% by weight of hydroxyapatite, 3-16% by weight of silk fibroin, 0.5-2% by weight of sodium alginate and 65-75% water by weight.

[0040] According to the method of the present invention, the preparation method of the biocompatible material comprises: a composite material of 15-32.5 parts by weight of silk fibroin and hydroxyapatite, 0.5-2 parts by weight of sodium alginate, 2-8 The silk fibroin protein in parts by weight and water in 65-75 parts by weight are mixed and stirred.

[0041] According to the met...

preparation example 1

[0072] Preparation of silk fibroin:

[0073] Prepare 1500ml of sodium carbonate solution with a mass fraction of 0.5% in a beaker, heat until the solution boils slightly, add 15g of silk, stir with a glass rod for 0.5h, rinse with deionized water until it is not sticky, repeat the above process for secondary degumming, degumming Then dry naturally. Take the weight after degumming. Then add 13.8g of degummed silk fibroin to the ternary system of calcium chloride, ethanol and water with a molar ratio of 1:2:8, dissolve at a constant temperature of 60°C for 2 hours, and add 1.5 times deionized water after dissolution (heated to 60°C), cooled to room temperature, filtered through a filter membrane, and put into a dialysis bag for dialysis for 3-5 days.

preparation example 2

[0075] Preparation of composite material of silk fibroin and hydroxyapatite (SF-HAP)

[0076] After soaking 15g of silk in 0.5% sodium carbonate solution (at a temperature of 90° C.) for 0.5 h by mass fraction, rinse with deionized water; silk fibroin. Weigh 3.25g of degummed silk fibroin and dissolve it in 36.5ml of a ternary system of calcium chloride, ethanol and water with a molar ratio of 1:2:8 at a temperature of 60°C; the degummed silk fibroin After complete dissolution, adjust the temperature to 75°C, and add diammonium hydrogen phosphate aqueous solution dropwise. The addition amount of diammonium hydrogen phosphate aqueous solution satisfies: based on Ca element in calcium chloride, based on P element in diammonium hydrogen phosphate aqueous solution, the consumption of diammonium hydrogen phosphate aqueous solution satisfies the molar ratio of Ca / P being 1.67. Along with continuously adding the diammonium hydrogen phosphate aqueous solution, white turbidity appear...

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Abstract

The invention relates to the field of bone tissue engineering scaffold materials, and discloses a bone scaffold material as well as a preparation method and application thereof. The preparation methodof the bone scaffold material comprises the following steps: (A) performing 3D direct-writing printing on a biocompatible material to obtain a bone scaffold structure; (B) molding, drying and modifying the obtained bone scaffold structure to obtain a bone scaffold material, wherein the biocompatible material is prepared from the following raw materials in percentage by weight: 12 to 26 percent ofhydroxyapatite, 3 to 16 percent of silk fibroin, 0.5 to 2 percent of sodium alginate and 65 to 75 percent of water. The prepared bone scaffold material has high water resistance and higher compression strength. The whole preparation process is carried out at room temperature without use of any organic solvents which are harmful to a human body. The bone scaffold material has high bioactivity andbiocompatibility, and the preparation method is simple and convenient.

Description

technical field [0001] The invention relates to the field of bone tissue engineering scaffold materials, in particular to a high-strength bone scaffold material and its preparation method and application. Background technique [0002] Bone defect repair has always been a problem in clinical orthopedics, and the development of bone tissue engineering has provided a new idea for bone defect repair. Bone tissue engineering scaffolds not only play a structural role in the body, but also play a role in cell adhesion, growth, reproduction, and provide a place for tissue regeneration and plasticity. Therefore, bone tissue engineering scaffold materials should have good biocompatibility, Degradability and osteoconductivity; certain mechanical strength and toughness; appropriate pore structure to meet the requirements of cell proliferation and differentiation on the surface of the material. At present, the research on scaffold materials for bone tissue engineering mainly focuses on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/12A61L27/20A61L27/22
Inventor 刘静李风煜田策宋延林
Owner INST OF CHEM CHINESE ACAD OF SCI
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