HPLC-MS/MS method for simultaneously measuring concentrations of three kinds of aminoglycoside antibiotics in plasma
A technology of aminoglycosides and drug concentration, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large detection limit, achieve good precision and accuracy, high throughput, and improve accuracy
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[0042] In this experiment, three aminoglycoside antibiotics (acamicin, isopamicin, gentamycin) standard substances and blank plasma were used to prepare a standard curve, and the drug concentration in the patient's plasma was calculated according to the standard curve.
[0043] 1. Experimental instruments and reagents are as follows:
[0044] Instrument: WATERS 2790 liquid-mass spectrometer, including WATERS 2790 high-performance liquid phase, MICROMASSQUATTRO triple quadrupole mass spectrometer, MASSLYNX 4.0 workstation, chromatographic column: Waters X Bridge HILIC column (4.6×50mm, 3.5μm), Xiangyi L550 centrifuges, shakers;
[0045] Reagents: formic acid methanol solution, methanol aqueous solution, amikacin standard, isopamicin standard, gentamicin standard, internal standard dabigatran standard.
[0046] 2. Experimental method
[0047] (1) Preparation of standard curve plasma samples and quality control plasma samples
[0048] 1.1 Preparation of stock solution
[0049...
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