HPLC-MS/MS method for simultaneously measuring concentrations of three kinds of aminoglycoside antibiotics in plasma

A technology of aminoglycosides and drug concentration, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large detection limit, achieve good precision and accuracy, high throughput, and improve accuracy

Active Publication Date: 2019-06-18
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the detection method of this patent and the detection method of this patent, the mass spectrometry parameters involved and other conditions are different, and the lower limit of detection of this patent is relatively large

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  • HPLC-MS/MS method for simultaneously measuring concentrations of three kinds of aminoglycoside antibiotics in plasma
  • HPLC-MS/MS method for simultaneously measuring concentrations of three kinds of aminoglycoside antibiotics in plasma
  • HPLC-MS/MS method for simultaneously measuring concentrations of three kinds of aminoglycoside antibiotics in plasma

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Embodiment 1

[0042] In this experiment, three aminoglycoside antibiotics (acamicin, isopamicin, gentamycin) standard substances and blank plasma were used to prepare a standard curve, and the drug concentration in the patient's plasma was calculated according to the standard curve.

[0043] 1. Experimental instruments and reagents are as follows:

[0044] Instrument: WATERS 2790 liquid-mass spectrometer, including WATERS 2790 high-performance liquid phase, MICROMASSQUATTRO triple quadrupole mass spectrometer, MASSLYNX 4.0 workstation, chromatographic column: Waters X Bridge HILIC column (4.6×50mm, 3.5μm), Xiangyi L550 centrifuges, shakers;

[0045] Reagents: formic acid methanol solution, methanol aqueous solution, amikacin standard, isopamicin standard, gentamicin standard, internal standard dabigatran standard.

[0046] 2. Experimental method

[0047] (1) Preparation of standard curve plasma samples and quality control plasma samples

[0048] 1.1 Preparation of stock solution

[0049...

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Abstract

The invention discloses an HPLC-MS/MS method for simultaneously measuring concentrations of three kinds of aminoglycoside antibiotics in plasma. According to the method, with Dabigatran as an interiorlabel, the protein in plasma is precipitated by using a methanol solution containing formic acid with the volume fraction of0.1%; drug components in the supernatant are separated by using high performance liquid chromatography; and then drug targeting detection is carried out by using a high-resolution mass spectrometry multi-reaction monitoring mode and quantification is performed, thereby realizing simultaneous analysis and measurement of the concentrations of three kinds of aminoglycoside antibiotics in plasma. The method has advantages of high speed, high targeting performance, rapidness,high throughput, high sensitivity, high specificity, high precision and accuracy, high stability, high extraction recovery rate, obvious matrix effect prevention, and dilution effect prevention. Themethod can be applied to the blood concentration monitoring of the conventional aminoglycoside antibiotics clinically, thereby guiding the rational clinical application of aminoglycoside antibiotics.

Description

technical field [0001] The invention relates to the technical field of clinical blood drug concentration monitoring and control, in particular to an HPLC-MS / MS method for simultaneously measuring the drug concentration of aminoglycoside antibiotics in blood plasma. Background technique [0002] Aminoglycoside antibiotics (Aminoglyeosides), such as amikacin, isopamicin, and gentamycin, are glycoside antibiotics composed of amino sugars and aminocyclic alcohols linked by oxygen bridges. Aminoglycoside antibiotics have a wide antibacterial spectrum, strong bactericidal activity, and obvious post-antibiotic effects, so they have a very important value in the treatment of severe nosocomial infections. However, these antibiotics are mainly excreted from the kidneys and have ear and kidney toxicity. The efficacy and toxicity of the drug are related to the blood concentration. The advantages and toxic effects of aminoglycoside antibiotics are extremely prominent. Therefore, clinici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
Inventor 陈明范国荣汪硕闻范先煜章越凡唐原君缪文清潘慧张令
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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