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Swine-borne lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and culture, applied in the field of microorganisms, which can solve the problems of slow acid rise in fermentation, generation of harmful gases or substances, corruption of raw materials, etc., and achieve the effects of prolonging the shelf life, high safety, and early fermentation start

Active Publication Date: 2019-07-05
GUIZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional silage is naturally fermented under anaerobic conditions, and generally has the following problems: the growth of lactic acid bacteria is slow, and the fermentation cycle is prolonged; it cannot be formed in a short time Predominant flora, slow acid rise in fermentation, a large number of anaerobic bacteria, resulting in nutrient loss; harmful gases or substances are produced, poor palatability; spoilage bacteria or molds grow in large numbers, causing raw materials to rot or mildew, reducing feed quality
However, most of these lactic acid bacteria come from the surface of plants, and their antibacterial activity, intestinal adaptability and safety are not clear, and generally have little inhibitory effect on acid-resistant bacteria and molds

Method used

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  • Swine-borne lactobacillus plantarum and application thereof
  • Swine-borne lactobacillus plantarum and application thereof
  • Swine-borne lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening and Identification of Plantarum Lactobacillus R-21 of the Present Invention

[0028] 1. Screening of Lactobacillus plantarum R-21

[0029] 1.1 Sample source

[0030] The bacterial strain provided by the invention is isolated from the intestinal mucosa of free-range pigs in remote mountainous areas of Bijie City, Guizhou Province. The sampled pigs grew robustly, never got sick during the growing period, and never fed any finished feed, live bacteria preparation or antibiotics.

[0031] 1.2 Separation and purification

[0032] The jejunum, ileum, cecum, colon and rectum of the slaughtered pigs were taken in sections, cut open with sterilized dissecting scissors, carefully removed the intestinal contents, and carefully scraped off the intestinal mucosal appendages with an alcohol-sterilized scalpel. Collected in a 5mL sterile centrifuge tube. Take 1g of mucosal appendage sample in 9mL sterilized normal saline, shake and mix well, and take 1mL diluen...

Embodiment 2

[0048] Growth performance and antibacterial test of embodiment 2 lactobacillus plantarum R-21

[0049] 1. Growth performance test

[0050] Take 5ml of R-21 liquid seeds, transfer to 500mL MRS liquid medium, and culture at 37°C for 36h, during which 20ml samples are taken every 4h. Among them, 1ml sample is used for serial dilution, take 10 -5 、10 -6 and 10 -7 200 μl of each of the three gradients was spread on the plate, and the number of viable bacteria was counted after culture, and the concentration of viable bacteria at each time point was calculated; the remaining samples were tested by a pen-type pH meter, centrifuged to take the supernatant, and placed at -20°C Save for later use. The result is as figure 1 As shown, the bacterial concentration was as high as 6.27×10 when cultured to 12h 9 cfu (colony forming unit, cfu) / ml remained relatively stable thereafter, and there was no obvious decline until 36 hours; during the cultivation process, the pH of the fermentati...

Embodiment 3

[0053] The safety test of embodiment 3 Lactobacillus plantarum R-21

[0054] Get the Lactobacillus plantarum R-21 fermented liquid cultivated in Example 2 for 32h, tank-feed 10 male adult Kunming mice of about 20g, each mouse 10 μ L / d, and continuously tank-feed for 28 days, during which each day quantitatively feeds about About 6g of basic feed. Another 10 mice were taken as controls, and fed with PBS solution 10 μL / mouse / d, and other operations were the same as those in the R-21 group. The body weight of the mice was recorded every 7 days, and any adverse reactions were observed. After the experiment, all the mice were killed, and the internal organs were dissected to observe whether there were lesions. The result is as image 3 As shown, the mice fed R-21 fermented liquid in tanks were fed with the same quality of feed, and the average weight gain was faster than that of the control group, and the two groups of test mice did not have any adverse reactions or lesions duri...

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Abstract

The invention discloses swine-borne lactobacillus plantarum and application thereof. The strain is named lactobacillus plantarum R-21(Lactobacillus plantarum R-21), is preserved in the China Center for Type Culture Collection, has the preservation number of CCTCC M2018009, and is preserved on January 8, 2018. The invention further discloses application of the strain in preparation of feed or silage. The lactobacillus plantarum grows quickly and has strong acid producing performance; and the fermentation broth of the strain has a remarkable inhibiting effect on aspergillus and common enteropathogenic bacteria and can be used in feed addition or fermented feed preparation.

Description

technical field [0001] The application belongs to the technical field of microorganisms, and in particular relates to a strain of Lactobacillus plantarum derived from pigs and its application. Background technique [0002] With the rapid development of the aquaculture industry, the demand for feed is increasing. The quality and cost of feed determine the profit of aquaculture, and it is one of the key factors that plague the development of the aquaculture industry. During the processing and storage of feed, due to the influence of operating conditions and environmental factors, it is easy to contaminate Escherichia coli, Salmonella, Staphylococcus aureus and Aspergillus flavus and other miscellaneous bacteria, resulting in a decline in feed quality, and in severe cases, animal infection, diarrhea, and poisoning , or even death etc. Adding an appropriate amount of antibiotics or non-biological chemical preservatives to animal diets can prolong the shelf life of feed, reduce ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12C12R1/25
CPCA23K10/12C12R2001/25C12N1/205A23V2400/169Y02P60/87
Inventor 夏品华曹海鹏严定波
Owner GUIZHOU NORMAL UNIVERSITY
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