Lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and microbial agent, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of cumbersome vaccine operation and development obstacles, improve immune function, reduce the content of ammonia nitrogen and nitrite, and inhibit bacteria significant effect

Pending Publication Date: 2019-07-12
江苏江心源生态农业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the cumbersome operation of vaccines, the development of vaccines has been hindered to a certain extent, and the immune booster is only limited to the immune enhancement of animal organisms, and has no qualitative help for water body changes.

Method used

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  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation and Identification of Embodiment 1 Lactobacillus plantarum

[0028] 1.1 Isolation and purification of bacterial strains

[0029] After the intestinal tissue homogenate of Chinese mitten crab from Jiangsu Jiangxinyuan Ecological Aquatic Products Co., Ltd. was diluted with sterile normal saline using a 10-fold dilution method, 10 -5 ~10 -8 Dilutions of the dilution range were spread on MRS solid medium plates. Incubate at a constant temperature of 30°C for 48 hours, pick a typical single colony, and repeatedly streak and isolate until a pure strain is obtained. A strain of lactic acid bacteria was isolated from the intestinal tract of Eriocheir sinensis by using MRS medium. The colony was milky white translucent protrusions with neat and smooth edges; Gram staining microscopic examination showed Gram-positive, rod-shaped, sour taste, and its number for CB5.

[0030] 1.2 Physiological and biochemical identification

[0031] The colony morphology of the strai...

Embodiment 2

[0043] The bacteriostasis of embodiment 2 Lactobacillus plantarum CB5 to aquatic animal pathogenic bacteria

[0044] 1. Preparation of cell-free fermentation supernatant:

[0045] After activated culture of Lactobacillus plantarum CB5, inoculate in MRS liquid medium with 4% inoculum amount, culture at 30°C until the stationary phase (36h-48h), centrifuge at 12000*g at 4°C for 15min, collect the supernatant, The supernatant was filtered with a 0.22 μm filter membrane to remove bacteria and other impurities to obtain a sterile fermentation supernatant. Store in a 4°C refrigerator for later use.

[0046] 2. Antibacterial activity test on aquatic pathogens:

[0047] In this study, the aquatic pathogen strains preserved in the laboratory were Aeromonas hydrophila TPS-30, BSK-10, Aeromonas sinensis CL99909, Staphylococcus aureus, Vibrio parahaemolyticus, Harvey Vibrio is the test strain, and the agar plate punching method is used: pour 10ml of heated and melted TSA medium (suitab...

Embodiment 3

[0052] The effect of embodiment 3 Lactobacillus plantarum CB5 on ammonia nitrogen and nitrite

[0053] 1 Materials and methods

[0054] 1.1 Lactobacillus plantarum

[0055] After activating and culturing Lactobacillus plantarum CB5, inoculate it in MRS liquid medium with 4% inoculation amount, and culture it statically at 30°C until it reaches a stable period (36h-48h). The number of bacteria is 5×10 9 cfu / ml.

[0056] 1.2 Chinese mitten crab

[0057] Bought from a farm in Huzhou City, with a body weight of 50.2±5g, they were domesticated with basal feed in a cement pond for 1 week before the experiment.

[0058] 1.3 The effect of oral Lactobacillus plantarum on ammonia nitrogen and nitrite in water

[0059] Add 1×10 to Chinese mitten crab powder feed 7 , 1×10 8 , 1×10 9 and 2.5×10 9 Cfu / g Lactobacillus plantarum CB5, dried at 40°C. After 1 week of adaptive breeding, Chinese mitten crabs were randomly divided into 5 groups and placed in aquaculture tanks with a water...

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Abstract

The invention discloses lactobacillus plantarum CB5. The CB5 is preserved in the China Center for Type Culture Collection, the deposit date is April 8, 2019, and the deposit number is CCTCC NO: M 2019244. The invention also discloses a microbial agent. The invention also discloses the lactobacillus plantarum CB5 and application of a microbial agent thereof, and the lactobacillus plantarum CB5 andthe microbial agent thereof have significant antibacterial activity against aquatic animal pathogenic bacteria, are used for improving the immune function of eriocheir sinensis, significantly reduce the content of ammonia nitrogen and nitrite in the water of cultured aquatic animals, and have the function of purifying water bodies.

Description

technical field [0001] The invention belongs to the technical field of aquaculture technology and ecological environment protection, and relates to a functional bacterium for reducing ammonia nitrogen, nitrite and antibacterial, in particular to a strain of Lactobacillus plantarum and its application. Background technique [0002] Chinese mitten crab (Eriocheir sinensis, commonly known as river crab) is a unique aquatic product in my country and one of the main aquaculture species for export earnings. It has been cultivated on a large scale in Jiangsu, Zhejiang, Anhui and other regions. In 2016, the output reached 81.2 mt. However, in intensive farming, due to the high protein content of the mitten crab feed, the rough farming model, the low protein utilization rate of the feed, and the characteristics of high nitrogen and phosphorus content in the farming wastewater. In a high-density breeding environment, the pollutants in the mitten crab breeding wastewater include residu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A61K35/747A61P37/04A61P31/04C12R1/25
CPCC02F3/34A61P37/04A61P31/04A61K2035/115C12R2001/25C12N1/205
Inventor 沈锦玉黄文江姚嘉赟蔺凌云潘晓艺尹文林马梁贺文庆
Owner 江苏江心源生态农业有限公司
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