Method for detecting nine mycotoxins in grains

Abstract

The invention discloses a method for detecting nine mycotoxins in grains. The method comprises the following steps of: taking grain samples, crushing and then sieving the grain samples to obtain grainsample powder; uniformly mixing acetonitrile and water as an extracting solution added into the grain sample powder, performing vortex extraction and then centrifugation, and collecting a supernate;taking the supernate injected into an EP tube, performing vortex extraction and then centrifugation, sucking out a supernatant, drying the supernatant by using nitrogen, dissolving the dried residue to perform filtering, and collecting the filtrate as the purified liquid of the samples to be detected; injecting the purified liquid of the samples to be detected into a liquid chromatograph, and performing detection by adopting liquid chromatography-mass spectrometry. The invention provides a simple, rapid and accurate one-time rapid screening and detection technology for mycotoxins in grains, the cost is low, the extraction speed is high, the operation is simple, the sensitivity is low, the recovery rate is good, the stability is high, and therefore, the method for detecting nine mycotoxinsin grains lays a technical foundation for defining the pollution and control of mycotoxins in grains and ensuring the quality safety of grains.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for detecting nine kinds of mycotoxins in grains. Background technique [0002] Mycotoxins are a class of toxic secondary metabolites produced by filamentous fungi under suitable environmental conditions, and are widely found in grains such as wheat and corn. The ingestion of food contaminated by mycotoxins by humans or animals may cause a variety of poisoning symptoms, which may endanger the lives of humans or animals in severe cases. There are more than 400 kinds of mycotoxins whose chemical structures have been confirmed, among which the most harmful to human health are deoxynivalenol (DON), 3-acetyl-deoxynivalenol (3ADON), 15-acetyl- Deoxynivalenol (15ADON), Nivalenol (NIV), Zearalenone (ZEN), Aflatoxin B 1 (AFB 1 ), aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ), aflatoxin G 2 (AFG 2 )wait. However, polytoxin pollution is common in grains. Therefore...

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Problems solved by technology

[0003] At present, the pretreatment methods of mycotoxins in grains mainly include thin-layer chromatography (TLC). Although this method does not have high requirements for instruments and the cost of detection is relatively low, it is generally only suitable for semi-quantitative, not suitable for Absolute quantitative analysis; solid phase extraction (SPE) is currently the main pretreatment method for the analysis of mycotoxins in grains. It has the advantages of relatively simple operation, good selectivity, and automatic batch processing, but the recovery rate of this method is low. , and the packing of the solid-phase extraction column is easily blocked; the immunoaffinity column purification method (IAC) has the advantages of strong selectivity and good sample purification effect, but its cost is expensive, the detection parameters are relatively single, and the operating mechanical strength is low; Immunosorbent assay (ELISA) has fast detection speed and strong selectivity, but it is cumbersome to operate and is easily interfered by non-target substances, resulting in false positives; liquid phase microextraction has the advantages of high enrichment efficiency and simple operation, but its stability is poor. Poor reusability; magnetic separation method has good enrichment effect, easy operation, certain mechanical strength and chemical stability, but poor selectivity; QuEChERS (Quick, Easy, Effective, Rugged, Safe) method uses adsorbent The filler interacts with the impurities in the matrix to achieve the purification purpose of removing impurities. Because of its fast analysis speed, easy operation, good recovery rate, and high safety, it has gradually become a common pretreatment method for mycotoxin detection in recent years.

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