Preparation method of photo-thermal reagent for fluorescence imaging mediated photo-thermal tumor treatment and application of photo-thermal reagent

A technology for tumor treatment and fluorescence imaging, which is applied in preparations for in vivo experiments, antineoplastic drugs, and medical preparations with non-active ingredients, etc. Dye and other issues

Inactive Publication Date: 2019-07-19
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Carbon dots are characterized by being easily soluble in water, low toxicity, environmental protection, low development cost, and biocompatibility. They are a new type of carbon-based zero-dimensional material and an ideal material for the preparation of new photothermal therapeutic ag

Method used

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  • Preparation method of photo-thermal reagent for fluorescence imaging mediated photo-thermal tumor treatment and application of photo-thermal reagent
  • Preparation method of photo-thermal reagent for fluorescence imaging mediated photo-thermal tumor treatment and application of photo-thermal reagent
  • Preparation method of photo-thermal reagent for fluorescence imaging mediated photo-thermal tumor treatment and application of photo-thermal reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This example is a better preparation method of carbon dots, thermosensitive liposome-carbon dot composite nanoparticles and thermosensitive liposome-indocyanine green-photothermal therapy carbon dot composite nanoparticles.

[0042] The carbon dots prepared in this example are near-infrared region two response carbon dots, which include the following preparation steps:

[0043] 1) Add 4g of pyrene to 320mL of concentrated nitric acid, condense and reflux at 80°C for 32h, continuously dilute the obtained mixture with deionized water to wash the acid in the solution, and finally use a 220nm filter to remove large particles, and then dry to obtain trinitrate Base pyrene powder.

[0044] 2) Take 0.1 g of trinitropyrene obtained in the first step, dissolve 1.6 g of polyethyleneimine in 10 mL of deionized water, stir and mix with trinitropyrene, put it into a microwave reactor, and react at 200 ° C for 1.5 min .

[0045] 3) Pass the black liquid obtained in step 2 through a...

Embodiment 2

[0054] This example is another preferred preparation method of the thermosensitive liposome-indocyanine green-photothermal therapy carbon dot composite nanoparticle of Example 1, which includes the following steps:

[0055] a) weighing dipalmitoyl lecithin (DPPC), hydrogenated soybean lecithin (HSPC), high-purity cholesterol (CHO), distearoyl phosphatidylethanolamine-polyethylene glycol 2000, indocyanine green molar ratio is 55: 20:20:2:2, the total phospholipid concentration is 10-40 mg / mL, add 5 mL of chloroform and 1 mL of methanol to dissolve. The organic solvent was then removed by rotary evaporation at 60 °C, and the resulting thin lipid film was further dried.

[0056] b) The obtained thin lipid film was treated with carbon dots (800 μg / mL) in (NH 4 ) 2 SO 4 The solution (10 mL, 200 mM) was hydrated at 50° C. for 1.5 hours. The solution was then sonicated through a bath at 50°C for 8 minutes to form a homogeneous suspension and extruded 60 times through a polycarbon...

Embodiment 3

[0059] The carbon dots prepared in Example 1, thermosensitive liposome-carbon dot composite nanoparticles and thermosensitive liposome-indocyanine green-photothermal therapy carbon dot composite nanoparticles are studied as follows:

[0060] (1) In vitro photothermal properties of carbon dots and thermosensitive liposome-carbon dot composite nanoparticles:

[0061] Dilute the carbon dots (or thermosensitive liposome-carbon dot composite nanoparticles) to different concentrations (0-500μg / mL, then transfer to a 1.5mL centrifuge tube, and use a 1064nm laser at 0.8W·cm -2 The power density was irradiated for 5 minutes, and the temperature change was recorded with an infrared thermal imager every 20s. Change the power (0.4-1W·cm -2 ) irradiate 500 μg / mL carbon dots (or thermosensitive liposome-carbon dot composite nanoparticles) with a 1064nm laser to determine the influence of different powers on the temperature rise of carbon dots. The passing power density is 0.8W / cm 2 The 1...

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Abstract

The invention relates to a preparation method of a photo-thermal reagent for fluorescence imaging mediated photo-thermal tumor treatment and an application of the photo-thermal reagent. The method comprises the steps of placing dipalmitoyl phosphatidylcholine, hydrogenated soybean phospholipid, high-purity cholesterol, DSPE-PEG2000 and indocyanine green in a chloroform and methanol mixed solutionfor dissolving so as to obtain a thin lipid membrane; and performing hydration, ultrasonic treatment, extrusion and removal of dissociated indocyanine green and carbon dots on the thin lipid membranein an ammonium sulfate solution containing carbon dots, and performing hydration, ultrasonic treatment, extrusion and dialysis in the ammonium sulfate solution containing carbon dots so as to obtain thermal-sensitive liposome-indocyanine green-carbon dot composite nanometer particles. The composite nanometer particles have obvious absorption in the range from a visible light region to a near infrared region, have obvious cytotoxicity under laser illumination, can effectively remain at tumors in a targeting manner for a long time so as to effectively treat tumors, do not have obvious pathologiclosses on main organs, and have high safety and high efficiency.

Description

technical field [0001] The invention belongs to the technical field of photothermal reagent preparation, and in particular relates to a preparation method and application of a photothermal reagent for photothermal tumor treatment mediated by fluorescence imaging. Background technique [0002] Near-infrared photothermal therapy agent refers to the use of reagents with high photothermal conversion efficiency in the near-infrared region, injecting them into the interior of organisms through blood vessels, using targeted recognition technology to gather near tumor tissues, and absorbing them under near-infrared light. A therapeutic agent that converts light energy into heat energy to kill cancer cells under direct irradiation. Common photothermal therapy agents include noble metal nanoparticles, carbon materials, metal and nonmetal compounds, and organic dyes. However, the current photothermal therapeutic agents have the characteristics of poor targeting and poor absorption in ...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K41/00A61K47/28A61K49/00A61P35/00
CPCA61K9/127A61K41/0052A61K47/28A61K49/0034A61K49/0054A61K49/0084A61P35/00
Inventor 沈龙祥潘登余耿弼江
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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