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Biological sponge based on acellular small intestinal submucosa and preparation method thereof

A small intestinal submucosa, decellularization technology, applied in tissue regeneration, drug delivery, pharmaceutical formulations, etc., can solve problems affecting clinical use, cytotoxicity, environmental pollution, etc., to achieve the growth of supporting cells, good mechanical properties, Beneficial to the effect of clinical operation

Inactive Publication Date: 2019-07-26
上海仁康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are certain problems in these methods at present: most of the current cross-linking agents are glutaraldehyde, EDC / NHS and other reagents, and these reagents themselves will pollute the environment, and the follow-up treatment will increase the cost
The most important thing is that the residue of the reagent will cause severe cytotoxicity, which will greatly affect the clinical use, and most of the products obtained through chemical crosslinking do not contain nutrients such as growth factors that are beneficial to the growth of tissue cells, so they cannot be used as an ideal drug. materials of biological origin

Method used

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  • Biological sponge based on acellular small intestinal submucosa and preparation method thereof
  • Biological sponge based on acellular small intestinal submucosa and preparation method thereof
  • Biological sponge based on acellular small intestinal submucosa and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] The embodiment of the present invention discloses a bio-sponge based on decellularized small intestinal submucosa. The bio-sponge uses SIS as a raw material and cross-links with fa-PEG to prepare a three-dimensional spatial structure rich in A new type of tissue engineering scaffold material with growth factors and no ethical issues.

[0046] The preparation method of biological sponge comprises the following steps:

[0047] (1) Preparation of decellularized porcine small intestinal submucosa (SIS)

[0048] Squeeze out the contents of the fresh porcine small intestine, and tear off the mesentery attached to the surface. Use scissors, scalpels, etc. to remove the muscular layer, mucosal layer and accompanying fat components of the small intestine, try to remove as much as possible to avoid residues, rinse with tap water repeatedly, and then rinse with distilled water for 3 times, each time for 15 minutes. Utilize 2% povidone iodine to clean and disinfect, use alcohol to ...

Embodiment 2

[0062] The preparation method of biological sponge comprises the following steps:

[0063] (1) Preparation of decellularized porcine small intestinal submucosa (SIS)

[0064] Squeeze out the contents of the fresh porcine small intestine, and tear off the mesentery attached to the surface. Use scissors, scalpels, etc. to remove the muscular layer, mucosal layer and accompanying fat components of the small intestine, try to remove as much as possible to avoid residues, rinse with tap water repeatedly, and then rinse with distilled water for 3 times, each time for 15 minutes. Utilize 2% povidone iodine to clean and disinfect, use alcohol to deiodine, and then soak and clean with sterile distilled water; then use 0.5mol / L sodium chloride solution to treat for 24 hours; discard the sodium chloride solution and wash pig small intestinal submucosa with a concentration of 5% SDS solution was shaken for 30 minutes; finally washed with saline to obtain SIS;

[0065] (2) Pepsin hydroly...

Embodiment 3

[0078] The preparation method of biological sponge comprises the following steps:

[0079] (1) Preparation of decellularized porcine small intestinal submucosa (SIS)

[0080] Squeeze out the contents of the fresh porcine small intestine, and tear off the mesentery attached to the surface. Use scissors, scalpels, etc. to remove the muscular layer, mucosal layer and accompanying fat components of the small intestine, try to remove as much as possible to avoid residues, rinse with tap water repeatedly, and then rinse with distilled water for 3 times, each time for 15 minutes. Use 2% iodophor to clean and disinfect, use alcohol to deiodine, and then soak and clean with sterile distilled water; then use 3mol / L sodium chloride solution for 12h; % SDS solution for 2 hours; finally washed with normal saline to obtain SIS;

[0081] (2) Pepsin hydrolysis

[0082] Add 5% pepsin solution to SIS, and stir at room temperature for 12 hours;

[0083] (3) cleaning

[0084] Filtrating the ...

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Abstract

The invention discloses a biological sponge based on the acellular small intestinal submucosa and a preparation method thereof. The preparation method comprises the steps of preparing the acellular porcine small intestinal submucosa, pepsin enzymatic hydrolysis, washing, lyophilization, pulverization and cross-linking. According to the preparation method, the porcine small intestinal submucosa istaken as a source; after the decellularization treatment, the immunological rejection is extremely small. At the same time, the cross-linking treatment is carried out by using a non-toxic cross-linking agent to ensure the biosafety of the obtained product. The product can be used not only for wound repair, but also as an excellent tissue engineering skin scaffold material. The product is sufficient in source, safe and reliable, cheap and easy to obtain. The composition and structure are similar to the humans. No ethical problems exist, and scale industrial preparation can be facilitated.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a biological sponge based on decellularized small intestinal submucosa and a preparation method. Background technique [0002] Burns, wounds, diabetes, chronic ulcers, etc. often lead to skin defects. Pain, loss of physical function, and changes in appearance caused by refractory, chronic trauma, and tissue defects seriously affect the patient's freedom of movement and quality of life, and further lead to psychological and social problems. Skin transplantation is the most effective treatment for tissue defects and skin pigmentation diseases, but it is limited by sources in clinical practice, which affects the treatment process. Tissue-engineered skin is the most promising treatment method. [0003] The current tissue-engineered skin scaffold materials mainly include chemically synthesized materials such as polylactic acid (PLA), poly-left-lactic acid (PLLA), etc. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/50A61L27/36
CPCA61L27/3629A61L27/3687A61L27/3691A61L27/50A61L27/60A61L2400/06A61L2430/40
Inventor 董亚兵朱宁文朱婷朱迎春
Owner 上海仁康科技有限公司
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