A kind of microbial deodorant bacteria agent
A deodorant bacterium and microorganism technology, applied in the field of microorganisms, can solve the problems of limited application range, inadaptability to high temperature environment, inapplicability, etc., and achieve the effect of improving safety
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Embodiment 1
[0026] NH 3 Selective medium: 50.0g sucrose, 10.0mL ammonia water, KH 2 PO 4 2.0 g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 0.1g, 1% ZnSO 4 5.0 mL, NaCl 2.0g, distilled water 1000mL, sterilize at 121°C for 15min.
[0027] h 2 S selective medium: glucose 5.0g, K 2 HPO 4 0.5g, KNO 3 1.0 g, MgCl 2 0.5 g, NaCl 0.5 g, NH 4 Cl 0.5g, Na 2 CO 3 1.0g, FeCl 2 0.01 g, distilled water 1000mL, pH natural, sterilized at 121°C for 15min.
[0028] Screening of deodorant bacteria:
[0029] 1) Weigh 10g of pig manure and 10mL of leachate, add 100mL of NH 3 and H 2 S selective culture medium, 30°C, 220r / min shaker culture, replace the medium after 2 days, draw 10mL of the acclimatization medium and add it to 100mL of fresh selective medium for the second generation of acclimatization, continuous enrichment and acclimatization 4 times. Take 10mL of the enrichment solution in a conical flask containing 90mL of sterile water, put it into a conical flask containing 90mL of sterile water, shake...
Embodiment 2
[0038] Medium formula: peptone 10g, beef extract 5g, glucose 5g, agar powder 20g, dilute to 1000mL, divide into 250mL Erlenmeyer flasks, 100mL per bottle. 121°C, 20min, high temperature and high pressure sterilization. Preparation of 0.05MPBS: Prepare solution A: Solution B at a ratio of 1:4, add 9.465g of sodium dihydrogen phosphate to 1000mL of solution A, add water to 9.27g of solution B, and add dipotassium hydrogen to 1000mL.
[0039] 1) Sample pretreatment: Weigh 5g of Bacillus flexus Z2 powder into a 500mL shaker flask with glass beads, then add 90mL of sterile water into the shaker flask, place the shaker flask on a shaker at 120rpm, and shake at 30°C Evenly, finally place the shaker flask in a 30°C water bath to keep warm for use, count the viable bacteria and record the number of bacteria in the shaker flask;
[0040] 2) Dilution of samples: Add 9 mL of PBS into 20 sterile test tubes respectively, mark the sample number and the number of dilution times on the test t...
Embodiment 3
[0058] Quantitative detection of the growth and deamination capacity of Bacillus flexus Z2 in the medium with ammonium chloride as the only nitrogen source:
[0059] Ammonium chloride as the only nitrogen source medium formula: sodium succinate 2.5g, sodium citrate dihydrate 2.5g, NH 4 Cl0.0636g, K 2 HPO 4 1g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.2g, pH 7.4, dilute to 1000mL, dispense into 250mL Erlenmeyer flasks, 100mL per bottle. 121°C, 20min, high temperature and high pressure sterilization. Add 1% of sterile-filtered complex carbon source. Compound carbon source: D-glucose 6.9g, D-fructose 6.9g, D-lactose 6.9g, 90% lactic acid 6.4mL, mannitol 7g, sodium acetate 9.5g, glycerin 6.3mL, absolute ethanol 7mL, salicylic acid 4.6 g, 4.8g of sodium benzoate, dissolved in 500mL of water, pH 7.4, sterilized by filtration with a 0.22μm filter membrane.
[0060] Inoculate Bacillus flexus Z2 into the above-mentioned culture medium, culture on a shaker at 30°C and 120rpm, take sample...
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