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Cultivation method of spirulina platensis in saline stress environment

A technology of spirulina platensis and cultivation method, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of affecting the yield and production cost of spirulina, increasing the difficulty and cost of harvesting, Affect the growth and development of spirulina, and achieve the effects of increasing biomass density and filtration and harvesting efficiency, increasing growth and harvesting benefits, increasing nutritional value and medicinal value

Active Publication Date: 2019-08-16
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Salinity is an important environmental factor that affects plant growth and yield. Under salt stress, the photosynthesis and respiration systems of Spirulina will be inhibited, which will affect the growth and development of Spirulina and the morphology of filaments. The harvest of spirulina will have a great impact. If the algal filaments are severely broken and a large number of short individuals appear, it will increase the difficulty and cost of harvesting, which will affect the yield and production cost of spirulina.

Method used

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  • Cultivation method of spirulina platensis in saline stress environment
  • Cultivation method of spirulina platensis in saline stress environment
  • Cultivation method of spirulina platensis in saline stress environment

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The cultivation method of Spirulina platensis under salt stress environment comprises: under the salt stress environment, carrying out the procedure of salt-tolerant acclimation to Spirulina; Place the Spirulina after acclimatization and cultivation in the environment where the partial pressure of oxygen rises for cell growth. The process of differentiation and cultivation; and the process of further expanding the cultivation and collecting the obtained salt-tolerant and oxygen-tolerant spirulina; the initial salt concentration in the above-mentioned salt stress environment is 1%, and the final concentration is 3.5%. The cultivation method utilizes the salt stress environment and the environment of rising oxygen partial pressure to domesticate and cultivate Spirulina, and can carry out stimulating cultivation under the conditions of the early cell development environment, so that the cells can produce their own defense characteristics and resistance substances, and then o...

Embodiment 2

[0041] 1) Take the crushed needle leaves of Taxus chinensis and pack them into Soxhlet extraction equipment, add 5 times the amount of petroleum ether, and keep warm at 80°C for 10h, then extract at 85°C for 8.5h, collect the extract, reduce Concentrate under pressure to obtain the extract, dissolve the extract with 2.5 times the amount of petroleum ether, add 5% sodium hydroxide methanol solution according to the ratio of solid to liquid 1:5, shake and hydrolyze at 780°C and 7250rpm / min for 2h , concentrated under reduced pressure to obtain the extract, which was dissolved in 6 times the amount of acetone, left standing at 4°C to remove impurities for 6.5 hours, concentrated under reduced pressure, the obtained extract was the extract polyprenol, the above hydrogenated The sodium methanol solution contains 0.07wt% pyrogallic acid and 0.05wt% cinnamic acid;

[0042] 2) Take the polypentenol extract and sodium 4-hydroxybutyrate, add 1 / 2 times the amount of Tween, 2 times the am...

Embodiment 3

[0048] The difference between this example and Example 2 is that no pyrogallic acid and cinnamic acid are added to the sodium hydroxide methanol solution for hydrolysis and decontamination in step 1);

[0049] The other steps were the same as in Example 2 to obtain salt-tolerant and oxygen-tolerant Spirulina.

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Abstract

The invention provides a cultivation method of spirulina platensis in a saline stress environment, and belongs to the field of biotechnology, which comprises the following steps: in the saline stressenvironment, performing salt-tolerant acclimation cultivation on the of spirulina platensis; placing the acclimated and cultivated spirulina in an environment with an elevated oxygen partial pressurefor cell differentiation cultivation; and performing further extended cultivation, and collecting the obtained salt-tolerant and oxygen-tolerant spirulina species. In the above saline stress environment, the initial salt concentration is 0.1-1%, the final concentration is 3.5-5%. The cultivation method provided in the invention may perform stimulant cultivation at an early stage of cells, which improves the algae superoxide dismutase activity, the peroxidase activity and the catalase activity, increases the algal filament thread pitch and the length in order to improve the biomass density andthe filtering and harvesting efficiency, reduces the malondialdehyde production, and promotes increase of algal phycocyanin and polysaccharide contents, facilitating industrial and oriented acquisition of nutrients including phycocyanins and polysaccharides.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a cultivation method of spirulina platensis under salt stress environment. Background technique [0002] Spirulina is a new resource microalgae with high development value. It is rich in algal polysaccharides, unsaturated fatty acids, phycobiliproteins, β-carotene, superoxide dismutase and various vitamins and other biologically active substances. The effect of health care, disease prevention and auxiliary treatment is natural health food and medicine source food. At present, there are more than 30 species of spirulina known in the world, and there are only 2 species of spirulina produced artificially and industrially at home and abroad, Spirulina maxima and Spirulina platensis. Compared with Spirulina platensis, Spirulina platensis has longer algae filaments and wider pitch, but in terms of nutritional content, Spirulina platensis has higher nutritional content ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/20C12R1/01
CPCC12N1/36C12N1/20Y02A40/80
Inventor 马玉心崔大练
Owner ZHEJIANG OCEAN UNIV
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